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| Construction of a high-density adzuki bean genetic map and evaluation of its utility based on a QTL analysis of seed size |
WANG Li-xia1, WANG Jie1, LUO Gao-ling2, YUAN Xing-xing3, GONG Dan1, HU Liang-liang1, WANG Su-hua1, CHEN Hong-lin1, CHEN Xin3, CHENG Xu-zhen1 |
1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2 Institute of Rice Research, Guangxi Academy of Agricultural Sciences, Nanning 530007, P.R.China
3 Institute of Economic Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, P.R.China |
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摘要
小豆(Vigna angularis (Willd.) Ohwi & Ohashi) 属于豇豆属亚洲豇豆亚属，是东亚各国传统种植作物。小豆营养丰富、医食两用，消费市场逐渐遍布全球。然而，小豆的遗传研究相对缓慢，导致育种技术落后、效率低下，难以满足生产和市场的需求。本研究基于高通量基因组测序技术构建了小豆SNP高密度遗传连锁图谱，该图谱共11条连锁群，含2904个标记，每条连锁群的标记数从208个（LG7）到397（LG1）个不等。图谱总长1365.0cM，标记间平均距离0.47cM，每条连锁群的长度从97.4cM（LG9）到155.6cM（LG1）不等。利用该图谱共发掘到两个与籽粒大小有关的主效QTL，分别位于LG2（22.1%） 和LG 9（18.8%）。此外，基于InDel侧翼序列，进一步开发了9718对引物，并随机选择200对进行PCR扩增检验，结果显示有75对在24份小豆种质中具有多态性。本研究中高密度图谱构建及籽粒大小的QTL 分析将进一步提升小豆重要性状基因的发掘等，而InDel标记的开发将有效促进小豆种质资源的遗传多样性分析、基因初步定位等研究。
Abstract Adzuki bean (Vigna angularis (Willd.) Ohwi & Ohashi) is an annual cultivated leguminous crop commonly grown in Asia and consumed worldwide. However, there has been limited research regarding adzuki bean genetics, which has prevented the efficient application of genes during breeding. In the present study, we constructed a high-density genetic map based on whole genome re-sequencing technology and validated its utility by mining QTLs related to seed size. Moreover, we analyzed the sequences flanking insertions/deletions (InDels) to develop a set of PCR-based markers useful for characterizing adzuki bean genetics. A total of 2 904 markers were mapped to 11 linkage groups (LGs). The total length of the map was 1 365.0 cM, with an average distance between markers of 0.47 cM. Among the LGs, the number of markers ranged from 208 (LG7) to 397 (LG1) and the total distance ranged from 97.4 cM (LG9) to 155.6 cM (LG1). Twelve QTLs related to seed size were identified using the constructed map. The two major QTLs in LG2 and LG9 explained 22.1 and 18.8% of the total phenotypic variation, respectively. Ten minor QTLs in LG4, LG5 and LG6 explained 3.0–10.4% of the total phenotypic variation. A total of 9 718 primer pairs were designed based on the sequences flanking InDels. Among the 200 selected primer pairs, 75 revealed polymorphisms in 24 adzuki bean germplasms. The genetic map constructed in this study will be useful for screening genes related to other traits. Furthermore, the QTL analysis of seed size and the novel markers described herein may be relevant for future molecular investigations of adzuki bean and will be useful for exploiting the mechanisms underlying legume seed development.
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Received: 25 December 2019
Accepted:
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| Fund: The study was supported by the National Key Research & Development Program of China (2019YFD1001300 and 2019YFD1001303), the earmarked fund for China Agriculture Research System (CARS-08) and the Agricultural Science Technology Innovation Program (ASTIP) of Chinese Academy of Agricultural Sciences. |
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Corresponding Authors:
Correspondence CHENG Xu-zhen, Tel: +86-10-62189159, E-mail: chengxuzhen@caas.cn
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| About author: WANG Li-xia, E-mail: wanglixia03@caas.cn |
Cite this article:
WANG Li-xia, WANG Jie, LUO Gao-ling, YUAN Xing-xing, GONG Dan, HU Liang-liang, WANG Su-hua, CHEN Hong-lin, CHEN Xin, CHENG Xu-zhen.
2021.
Construction of a high-density adzuki bean genetic map and evaluation of its utility based on a QTL analysis of seed size. Journal of Integrative Agriculture, 20(7): 1753-1761.
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