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Journal of Integrative Agriculture  2020, Vol. 19 Issue (7): 1721-1730    DOI: 10.1016/S2095-3119(20)63224-5
Special Issue: Triticeae Crops Genetics · Breeding · Germplasm Resources
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Genetic analysis and QTL mapping of a novel reduced height gene in common wheat (Triticum aestivum L.)
ZHOU Chun-yun1, 2*, XIONG Hong-chun2*, LI Yu-ting2, GUO Hui-jun2, XIE Yong-dun2, ZHAO Lin-shu2, GU Jia-yu2, ZHAO Shi-rong2, DING Yu-ping2, SONG Xi-yun1, LIU Lu-xiang2 
1 School of Life Sciences, Qingdao Agricultural University, Qingdao 266109, P.R.China
2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Engineering Laboratory for Crop Molecular Breeding/National Center of Space Mutagenesis for Crop Improvement, Beijing 100081, P.R.China
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Abstract  
Low stature in wheat is closely associated with lodging resistance, and this impacts harvest index and grain yield.  The discovery of novel dwarfing or semi-dwarfing genes can have great significance for dwarf wheat breeding.  In this study, we identified an EMS-induced dwarf wheat mutant JE0124 from the elite cultivar Jing411.  JE0124 possesses increased stem strength and a 33% reduction in plant height compared with wild type.  Gibberellic acid (GA) treatment analysis suggested that JE0124 was GA-sensitive.  Analysis of the frequency distribution of plant height in four F2 populations derived from crosses between JE0124 and the relatively taller varieties Nongda 5181 and WT indicated that the dwarfism phenotype was quantitatively inherited.  We used two F2 populations and 312 individuals from the reciprocal cross of Nongda 5181 and JE0124 to map the quantitative trait locus (QTL) for reduced height to a 0.85-cM interval on chromosome 2DL.  The mapping was done by using a combination of 660K SNP array-based bulked segregant analysis (BSA) and genetic linkage analysis, with logarithm of odds (LOD) scores of 5.34 and 5.78, respectively.  Additionally, this QTL accounted for 8.27–8.52% of the variation in the phenotype.  The dwarf mutant JE0124 and the newly discovered dwarfing gene on chromosome 2DL in this study will enrich genetic resources for dwarf wheat breeding.
 
Keywords:  reduced height gene        wheat        QTL        BSA        molecular marker   
Received: 24 March 2019   Accepted: 24 May 2020
Fund: This work was financially supported by the National Key Research and Development Program of China (2016YFD0102100 and 2016YFD0101802), the National Natural Science Foundation of China (31801346) and the earmarked fund for China Agriculture Research System (CARS-03).
Corresponding Authors:  Correspondence LIU Lu-xiang, E-mail: liuluxiang@caas.cn   
About author:  * These authors contributed equally to this study.

Cite this article: 

ZHOU Chun-yun, XIONG Hong-chun, LI Yu-ting, GUO Hui-jun, XIE Yong-dun, ZHAO Lin-shu, GU Jiayu, ZHAO Shi-rong, DING Yu-ping, SONG Xi-yun, LIU Lu-xiang. 2020. Genetic analysis and QTL mapping of a novel reduced height gene in common wheat (Triticum aestivum L.). Journal of Integrative Agriculture, 19(7): 1721-1730.

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