Please wait a minute...
Journal of Integrative Agriculture  2020, Vol. 19 Issue (7): 1721-1730    DOI: 10.1016/S2095-3119(20)63224-5
Special Issue: 麦类遗传育种合辑Triticeae Crops Genetics · Breeding · Germplasm Resources
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Genetic analysis and QTL mapping of a novel reduced height gene in common wheat (Triticum aestivum L.)
ZHOU Chun-yun1, 2*, XIONG Hong-chun2*, LI Yu-ting2, GUO Hui-jun2, XIE Yong-dun2, ZHAO Lin-shu2, GU Jia-yu2, ZHAO Shi-rong2, DING Yu-ping2, SONG Xi-yun1, LIU Lu-xiang2 
1 School of Life Sciences, Qingdao Agricultural University, Qingdao 266109, P.R.China
2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Engineering Laboratory for Crop Molecular Breeding/National Center of Space Mutagenesis for Crop Improvement, Beijing 100081, P.R.China
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
Abstract  
Low stature in wheat is closely associated with lodging resistance, and this impacts harvest index and grain yield.  The discovery of novel dwarfing or semi-dwarfing genes can have great significance for dwarf wheat breeding.  In this study, we identified an EMS-induced dwarf wheat mutant JE0124 from the elite cultivar Jing411.  JE0124 possesses increased stem strength and a 33% reduction in plant height compared with wild type.  Gibberellic acid (GA) treatment analysis suggested that JE0124 was GA-sensitive.  Analysis of the frequency distribution of plant height in four F2 populations derived from crosses between JE0124 and the relatively taller varieties Nongda 5181 and WT indicated that the dwarfism phenotype was quantitatively inherited.  We used two F2 populations and 312 individuals from the reciprocal cross of Nongda 5181 and JE0124 to map the quantitative trait locus (QTL) for reduced height to a 0.85-cM interval on chromosome 2DL.  The mapping was done by using a combination of 660K SNP array-based bulked segregant analysis (BSA) and genetic linkage analysis, with logarithm of odds (LOD) scores of 5.34 and 5.78, respectively.  Additionally, this QTL accounted for 8.27–8.52% of the variation in the phenotype.  The dwarf mutant JE0124 and the newly discovered dwarfing gene on chromosome 2DL in this study will enrich genetic resources for dwarf wheat breeding.
 
Keywords:  reduced height gene        wheat        QTL        BSA        molecular marker   
Received: 24 March 2019   Accepted:
Fund: This work was financially supported by the National Key Research and Development Program of China (2016YFD0102100 and 2016YFD0101802), the National Natural Science Foundation of China (31801346) and the earmarked fund for China Agriculture Research System (CARS-03).
Corresponding Authors:  Correspondence LIU Lu-xiang, E-mail: liuluxiang@caas.cn   
About author:  * These authors contributed equally to this study.

Cite this article: 

ZHOU Chun-yun, XIONG Hong-chun, LI Yu-ting, GUO Hui-jun, XIE Yong-dun, ZHAO Lin-shu, GU Jiayu, ZHAO Shi-rong, DING Yu-ping, SONG Xi-yun, LIU Lu-xiang. 2020. Genetic analysis and QTL mapping of a novel reduced height gene in common wheat (Triticum aestivum L.). Journal of Integrative Agriculture, 19(7): 1721-1730.

Appels R, Eversole K, Feuillet C, Keller B, Rogers J, Stein N, Pozniak C J, Stein N, Choulet F. 2018. Shifting the limits in wheat research and breeding using a fully annotated reference genome. Science, 361, 6403.
Asplund L, Leino M W, Hagenblad J. 2012. Allelic variation at the Rht8 locus in a 19th century wheat collection. The Scientific World Journal, 2, 385610.
Bazhenov M S, Divashuk M G, Amagai Y, Watanabe N, Karlov G I. 2015. Isolation of the dwarfing Rht-B1p (Rht17) gene from wheat and the development of an allele-specific PCR marker. Molecular Breeding, 11, 213.
Berry P M, Spink J H, Gay A P, Craigon J. 2003. A comparison of root and stem lodging risks among winter wheat cultivars. The Journal of Agricultural Science, 2, 191–202.
Berry P M, Sterling M, Spink J H, Baker C J, Sylvester-Bradley R, Mooney S J, Tams A R, Ennos A R. 2004. Understanding and reducing lodging in cereals. Advances in Agronomy, 84, 217–271.
Berry P M, Sylvester-Bradley R, Berry S. 2006. Ideotype design for lodging-resistant wheat. Euphytica, 154, 165–179.
Borner A, Roder M, Korzun V. 1997. Comparative molecular mapping of GA insensitive Rht loci on chromosomes 4B and 4D of common wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 95, 1133–1137.
Casebow R, Hadley C, Uppal R, Addisu M, Loddo S, Kowalski A, Griffiths S, Gooding M. 2016. Reduced height (Rht) alleles affect wheat grain quality. PLoS ONE, 5, e0156056.
Chaudhry A S. 1973. A genetic and cytogenetic study of height in wheat. Ph D thesis, University of Cambridge, Cambridge.
Chen S, Gao R, Wang H, Wen M, Jin X, Bian N, Zhang R, Hu W, Cheng S. 2015. Characterization of a novel reduced height gene (Rht23) regulating panicle morphology and plant architecture in bread wheat. Euphytica, 3, 583–594.
Daoura B G, Chen L, Du Y, Hu Y G. 2014. Genetic effects of dwarfing gene Rht-5 on agronomic traits in common wheat (Triticum aestivum L.) and QTL analysis on its linked traits. Field Crops Research, 2, 22–29.
Daoura B G, Chen L, Hu Y. 2013. Agronomic traits affected by dwarfing gene Rht-5 in common wheat (Triticum aestivum L.). Australian Journal of Crop Science, 9, 1270–1276.
Divashuk M G, Bespalova L A, Vasilyev A V, Fesenko I A, Puzyrnaya O Y, Karlov G I. 2012a. Reduced height genes and their importance in winter wheat cultivars grown in southern Russia. Euphytica, 1, 137–144.
Divashuk M G, Vasilyev A V, Bespalova L A, Karlov G I. 2012b. Identity of the Rht-11 and Rht-B1e reduced plant height genes. Russian Journal of Genetics, 7, 761–763.
Du Y, Chen L, Wang Y, Yang Z, Saeed I, Daoura B G, Hu Y G. 2018. The combination of dwarfing genes Rht4 and Rht8 reduced plant height, improved yield traits of rainfed bread wheat (Triticum aestivum L.). Field Crops Research, 215, 149–155.
Duwayri M, Nguyen V N. 1999. Reflections on yield gaps in rice production. IRC Newsletters, 48, 13–26.
Ellis M H, Rebetzke G J, Azanza F, Richards R A, Spielmeyer W. 2005. Molecular mapping of gibberellin-responsive dwarfing genes in bread wheat. Theoretical and Applied Genetics, 3, 423–430.
Ellis M H, Rebetzke G J, Chandler P, Bonnett D, Spielmeyer W, Richards R A. 2004. The effect of different height reducing genes on the early growth of wheat. Functional Plant Biology, 6, 583.
Ford B A, Foo E, Sharwood R, Karafiatova M, Vrana J, MacMillan C, Nichols D S, Steuernagel B, Uauy C. 2018. Rht18 semidwarfism in wheat is due to increased GA 2-oxidaseA9 expression and reduced GA content. Plant Physiology, 1, 168–180.
Foulkes M J, Slafer G A, Davies W J, Berry P M, Sylvester-Bradley R, Martre P, Calderini D F, Griffiths S, Reynolds M P. 2011. Raising yield potential of wheat. III. Optimizing partitioning to grain while maintaining lodging resistance. Journal of Experimental Botany, 2, 469–486.
Gale M D, Youssefian S, Russell G E. 1985. Dwarfing genes in wheat. In: Progress in Plant Breeding-1. Butterworths, London, UK. pp. 1–35.
Gasperini D, Greenland A, Hedden P, Dreos R, Harwood W, Griffiths S. 2012. Genetic and physiological analysis of Rht8 in bread wheat: an alternative source of semi-dwarfism with a reduced sensitivity to brassinosteroids. Journal of Experimental Botany, 12, 4419–4436.
Grant N P, Mohan A, Sandhu D, Gill K S. 2018. Inheritance and genetic mapping of the reduced height (Rht18) gene in wheat. Plants, 3, 58.
Haque M, Martinek P, Watanabe N, Kuboyama T. 2011. Genetic mapping of gibberellic acid-sensitive genes for semi-dwarfism in durum wheat. Cereal Research Communications, 2, 171–178.
Hedden P. 2003. The genes of the Green Revolution. Trends in Genetic, 1, 5–9.
Islam M S, Peng S, Visperas R M, Ereful N, Bhuiya M S U, Julfiquar A W. 2007. Lodging-related morphological traits of hybrid rice in a tropical irrigated ecosystem. Field Crops Research, 2, 240–248.
Kashiwagi T, Ishimaru K. 2004. Identification and functional analysis of a locus for improvement of lodging resistance in rice. Plant Physiology, 2, 676–683.
Li Y Y, Xiao J H, Wu J, Duan J L, Liu Y, Ye X G, Zhang X, Guo X P, Gu Y Q, Zhang L C, Jia J Z, Kong X Y. 2012. A tandem segmental duplication (TSD) in green revolution gene Rht-D1b region underlies plant height variation. New Phytologist, 1, 282–291.
Mo Y, Vanzetti L S, Hale I, Spagnolo E J, Guidobaldi F, Al-Oboudi J, Odle N, Pearce S, Helguera M. 2018. Identification and characterization of Rht25, a locus on chromosome arm 6AS affecting wheat plant height, heading time, and spike development. Theoretical and Applied Genetics, 131, 2021–2035.
Noor R B M, Caviness C E. 1980. Influence of induced lodging on pod distribution and seed yield in soybeans. Agronomy Journal, 72, 904–906.
Peng J, Richards D E, Hartley N M, Murphy G P, Devos K M, Flintham J E. 1999. ‘Green revolution’ genes encode mutant gibberellin response modulators. Nature, 400, 256–2611.
Pinthus M J, Levy A A. 1983. The relationship between the Rht1 and Rht2 dwarfing genes and grain weight in Triticum aestivum L. spring wheat. Theoretical and Applied Genetics. 2, 153–157.
Rebetzke G J, Appels R, Morrison A D, Richards R A, Mcdonald G K, Ellis M H. 2001. Quantitative trait loci on chromosome 4B for coleoptile length and early vigour in wheat (Triticum aestivum L.). Australian Journal of Agricultural Research, 12, 1221–1234.
Rebetzke G J, Ellis M H, Bonnett D G, Condon A G, Falk D, Richards R A. 2011. The Rht13 dwarfing gene reduces peduncle length and plant height to increase grain number and yield of wheat. Field Crops Research, 3, 323–331.
Rebetzke G J, Ellis M H, Bonnett D G, Mickelson B, Condon A G, Richards R A. 2012. Height reduction and agronomic performance for selected gibberellin-responsive dwarfing genes in bread wheat (Triticum aestivum L.). Field Crops Research, 126, 87–96.
Rebetzke G J, Richards R A, Fischer V M, Mickelson B J. 1999. Breeding long coleoptile, reduced height wheats. Euphytica, 106, 159–168.
Sun L, Yang W, Li Y, Shan Q, Ye X, Wang D, Yu K, Lu W, Xin P. 2019. A wheat dominant dwarfing line with Rht12 which reduces stem cell length and affects GA synthesis, is a 5AL terminal deletion line. The Plant Journal, 97, 887–900.
Tan M K, Koval J, Ghalayini A. 2013. Novel genetic variants of GA-insensitive Rht-1 genes in hexaploid wheat and their potential agronomic value. PLoS ONE, 7, e69690.
Tang N, Jiang Y, He B R, Hu Y G. 2009. The effects of dwarfing genes (Rht-B1b, Rht-D1b, and Rht8) with different sensitivity to GA3 on the coleoptile length and plant height of wheat. Agricultural Sciences in China, 9, 1028–1038.
Tian X, Wen W, Xie L, Fu L, Xu D, Fu C, Wang D, Chen X, Xia X. 2017. Molecular mapping of reduced plant height gene Rht24 in bread wheat. Frontiers in Plant Science, 8, 1379.
Tripathi S C, Sayre K D, Kaul J N, Narang R S. 2004. Lodging behavior and yield potential of spring wheat (Triticum aestivum L.): Effects of ethephon and genotypes. Field Crops Research, 2, 207–220.
Vikhe P, Patil R, Chavan A, Oak M, Tamhankar S. 2017. Mapping gibberellin-sensitive dwarfing locus Rht18 in durum wheat and development of SSR and SNP markers for selection in breeding. Molecular Breeding, 37, 28.
Wen W, Deng Q, Jia H, Wei L, Wei J, Wan H, Yang L, Cao W, Ma Z. 2013. Sequence variations of the partially dominant DELLA gene Rht-B1c in wheat and their functional impacts. Journal of Experimental Botany, 11, 3299–3312.
Wojciechowski T, Gooding M J, Ramsay L, Gregory P J. 2009. The effects of dwarfing genes on seedling root growth of wheat. Journal of Experimental Botany, 9, 2565–2573.
Wu J, Kong X, Wan J, Liu X, Zhang X, Guo X, Zhou R, Zhao G, Jing R. 2011. Dominant and pleiotropic effects of a GAI gene in wheat results from a lack of interaction between DELLA and GID1. Plant Physiology, 4, 2120–2130.
Xiong H, Guo H, Xie Y, Zhao L, Gu J, Zhao S, Li J, Liu L. 2018. Enhancement of dwarf wheat germplasm with high-yield potential derived from induced mutagenesis. Plant Genetic Resources (Characterization and Utilization), 16, 74–81.
Xu C, Gao Y, Tian B, Ren J, Meng Q, Wang P. 2017. Effects of EDAH, a novel plant growth regulator, on mechanical strength, stalk vascular bundles and grain yield of summer maize at high densities. Field Crops Research, 200, 71–79.
Xue J, Gou L, Zhao Y, Yao M, Yao H, Tian J, Zhang W. 2016. Effects of light intensity within the canopy on maize lodging. Field Crops Research, 188, 133–141.
Yang S, Zhang X, He Z, Xia X, Zhou Y. 2006. Distribution of dwarfing genes Rht-B1b and Rht-D1b in chinese bread wheats detected by STS marker. Scientia Agricultura Sinica, 8, 1680–1688. (in Chinese)
Yang Z, Zheng J, Liu C, Wang Y, Condon A G, Chen Y, Hu Y. 2015. Effects of the GA-responsive dwarfing gene Rht18 from tetraploid wheat on agronomic traits of common wheat. Field Crops Research, 183, 92–101.
Zhang Q, Zhang L, Evers J, Werf W, Zhang W, Duan L. 2014. Maize yield and quality in response to plant density and application of a novel plant growth regulator. Field Crops Research, 164, 82–89.
[1] Changqin Yang, Xiaojing Wang, Jianan Li, Guowei Zhang, Hongmei Shu, Wei Hu, Huanyong Han, Ruixian Liu, Zichun Guo.

Straw return increases crop production by improving soil organic carbon sequestration and soil aggregation in a long-term wheat–cotton cropping system [J]. >Journal of Integrative Agriculture, 2024, 23(2): 669-679.

[2] Simin Liao, Zhibin Xu, Xiaoli Fan, Qiang Zhou, Xiaofeng Liu, Cheng Jiang, Liangen Chen, Dian Lin, Bo Feng, Tao Wang.

Genetic dissection and validation of a major QTL for grain weight on chromosome 3B in bread wheat (Triticum aestivum L.) [J]. >Journal of Integrative Agriculture, 2024, 23(1): 77-92.

[3] TU Ke-ling, YIN Yu-lin, YANG Li-ming, WANG Jian-hua, SUN Qun. Discrimination of individual seed viability by using the oxygen consumption technique and headspace-gas chromatography-ion mobility spectrometry[J]. >Journal of Integrative Agriculture, 2023, 22(3): 727-737.
[4] HU Wen-jing, FU Lu-ping, GAO De-rong, LI Dong-sheng, LIAO Sen, LU Cheng-bin. Marker-assisted selection to pyramid Fusarium head blight resistance loci Fhb1 and Fhb2 in a high-quality soft wheat cultivar Yangmai 15[J]. >Journal of Integrative Agriculture, 2023, 22(2): 360-370.
[5] ZHANG Guang-xin, ZHAO De-hao, FAN Heng-zhi, LIU Shi-ju, LIAO Yun-cheng, HAN Juan. Combining controlled-release urea and normal urea with appropriate nitrogen application rate to reduce wheat stem lodging risk and increase grain yield and yield stability[J]. >Journal of Integrative Agriculture, 2023, 22(10): 3006-3021.
[6] HUANG Feng, LI Xuan-shuang, DU Xiao-yu, LI Shun-cheng, LI Nan-nan, LÜ Yong-jun, ZOU Shao-kui, ZHANG Qian, WANG Li-na, NI Zhong-fu, HAN Yu-lin, XING Jie-wen. SNP-based identification of QTLs for thousand-grain weight and related traits in wheat 8762/Keyi 5214 DH lines[J]. >Journal of Integrative Agriculture, 2023, 22(10): 2949-2960.
[7] GAO Ri-xin, HU Ming-jian, ZHAO Hai-ming, LAI Jin-sheng, SONG Wei-bin.

Genetic dissection of ear-related traits using immortalized F2 population in maize [J]. >Journal of Integrative Agriculture, 2022, 21(9): 2492-2507.

[8] CHAO Kai-xiang, WU Cai-juan, LI Juan, WANG Wen-li, WANG Bao-tong, LI Qiang. Genetic analysis of adult plant, quantitative resistance to stripe rust in wheat landrace Wudubaijian in multi-environment trials[J]. >Journal of Integrative Agriculture, 2022, 21(8): 2305-2318.
[9] LI Si-ping, ZENG Lu-sheng, SU Zhong-liang. Wheat growth, photosynthesis and physiological characteristics under different soil Zn levels[J]. >Journal of Integrative Agriculture, 2022, 21(7): 1927-1940.
[10] LIU Chen, TIAN Yu, LIU Zhang-xiong, GU Yong-zhe, ZHANG Bo, LI Ying-hui, NA Jie, QIU Li-juan. Identification and characterization of long-InDels through whole genome resequencing to facilitate fine-mapping of a QTL for plant height in soybean (Glycine max L. Merr.)[J]. >Journal of Integrative Agriculture, 2022, 21(7): 1903-1912.
[11] JIANG Xue-qian, ZHANG Fan, WANG Zhen, LONG Rui-cai, LI Ming-na, HE Fei, YANG Xi-jiang, YANG Chang-fu, JIANG Xu, YANG Qing-chuan, WANG Quan-zhen, KANG Jun-mei. Detection of quantitative trait loci (QTL) associated with spring regrowth in alfalfa (Medicago sativa L.)[J]. >Journal of Integrative Agriculture, 2022, 21(3): 812-818.
[12] ZHANG Hai-feng, Tofazzal ISLAM, LIU Wen-de. Integrated pest management programme for cereal blast fungus Magnaporthe oryza[J]. >Journal of Integrative Agriculture, 2022, 21(12): 3420-3433.
[13] OCHAR Kingsley, SU Bo-hong, ZHOU Ming-ming, LIU Zhang-xiong, GAO Hua-wei, SOBHI F. Lamlom, QIU Li-juan. Identification of the genetic locus associated with the crinkled leaf phenotype in a soybean (Glycine max L.) mutant by BSA-Seq technology[J]. >Journal of Integrative Agriculture, 2022, 21(12): 3524-3539.
[14] JIA Jia, WANG Huan, CAI Zhan-dong, WEI Ru-qian, HUANG Jing-hua, XIA Qiu-ju, XIAO Xiao-hui, MA Qi-bin, NIAN Hai, CHENG Yan-bo. Identification and validation of stable and novel quantitative trait loci for pod shattering in soybean [Glycine max (L.) Merr.][J]. >Journal of Integrative Agriculture, 2022, 21(11): 3169-3184.
[15] SHI Mei-qi, LIAO Xi-liang, YE Qian, ZHANG Wei, LI Ya-kai, Javaid Akhter BHAT, KAN Gui-zhen, YU De-yue. Linkage and association mapping of wild soybean (Glycine soja) seeds germinating under salt stress[J]. >Journal of Integrative Agriculture, 2022, 21(10): 2833-2847.
No Suggested Reading articles found!