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Journal of Integrative Agriculture  2018, Vol. 17 Issue (07): 1563-1573    DOI: 10.1016/S2095-3119(17)61847-1
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Identification of QTLs associated with cadmium concentration in rice grains
HU Da-wei1*, SHENG Zhong-hua1*, LI Qian-long2, CHEN Wei2, WEI Xiang-jin1, XIE Li-hong1, JIAO Gui-ai1, SHAO Gao-neng1, WANG Jian-long2, TANG Shao-qing1, HU Pei-song1, 2 
1 State Key Laboratory of Rice Biology, Ministry of Science and Technology/Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture/China National Rice Research Institute, Hangzhou 310006, P.R.China
2 College of Agronomy, Hunan Agricultural University, Changsha 410128, P.R.China  
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Cadmium (Cd) contamination in rice has been a hot topic of research because of its potential risk to human health.  In this study, a double haploid (DH) population derived from Zhongjiazao 17 (YK17) (an early-season indica cultivar)×D50 (a tropical japonica cultivar) was used to identify quantitative trait loci (QTLs) associated with Cd concentration in brown rice (CCBR) and Cd concentration in milled rice (CCMR).  Continuous and wide variation for CCBR and CCMR were observed among the DH population.  Correlation analysis revealed a positive and highly significant correlation between the two traits.  A total of 18 QTLs for CCBR and 14 QTLs for CCMR were identified in five different pot and field trials.  Two pairs of QTLs for CCBR (qCCBR2-1 and qCCBR2-2, qCCBR9-1 and qCCBR9-2) and one pair of QTLs for CCMR (qCCMR5-1 and qCCMR5-2) were detected in multiple trials.  The alleles increasing CCBR at the qCCBR2-1/qCCBR2-2 and qCCBR9-1/qCCBR9-2 QTLs were contributed by YK17 and D50, respectively, whereas the D50 allele at the qCCMR5-1/qCCMR5-2 QTLs increased CCMR.  Eight pairs of QTLs for CCBR and CCMR, qCCBR2-2 and qCCMR2-2, qCCBR3 and qCCMR3, qCCBR4-2 and qCCMR4-1, qCCBR4-3 and qCCMR4-2, qCCBR4-4 and qCCMR4-3, qCCBR5 and qCCMR5-2, qCCBR7 and qCCMR7, and qCCBR11-1 and qCCMR11-2, co-localized on chromosomes 2, 3, 4, 5, 7, and 11, respectively.  For all of these QTL pairs, except qCCBR5/qCCMR5-2, the additive effects came from YK17.  In addition, four CCMR QTLs showing significant additive×environment interaction and two pairs of CCMR QTLs with bi-allelic epistatic interactions were identified.  The results of this study could facilitate marker-assisted selection of breeding rice varieties with low Cd accumulation in grain.
Keywords:  Cd concentration in grain ')" href="#">  
Received: 12 June 2017   Accepted:
Fund: This research was financially supported by the National Natural Science Foundation of China (31501285), the Zhejiang Science and Technology Projects, China (2015C32045), the National Key Research and Development Program of China (2016YFD0101801), and the National S&T Major Project, China (2016ZX08001006).
Corresponding Authors:  Correspondence HU Pei-song, Tel/Fax: +86-571-63370221, E-mail:,    
About author:  HU Da-wei, E-mail:; * These authors contributed equally to this study.
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HU Da-wei
SHENG Zhong-hua
LI Qian-long
WEI Xiang-jin
XIE Li-hong
JIAO Gui-ai
SHAO Gao-neng
WANG Jian-long
TANG Shao-qing
HU Pei-song

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HU Da-wei, SHENG Zhong-hua, LI Qian-long, CHEN Wei, WEI Xiang-jin, XIE Li-hong, JIAO Gui-ai, SHAO Gao-neng, WANG Jian-long, TANG Shao-qing, HU Pei-song. 2018. Identification of QTLs associated with cadmium concentration in rice grains. Journal of Integrative Agriculture, 17(07): 1563-1573.

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