Please wait a minute...
Journal of Integrative Agriculture  2019, Vol. 18 Issue (3): 526-531    DOI: 10.1016/S2095-3119(18)61996-3
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Identification and QTL mapping of Z550, a rice backcrossed inbred line with increased grains per panicle
WANG Shi-ming, CUI Guo-qing, WANG Hui, MA Fu-ying, XIA Sai-sai, LI Yun-feng, YANG Zheng-lin, LING Ying-hua, ZHANG Chang-wei, HE Guang-hua, ZHAO Fang-ming
Rice Research Institute, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, P.R.China
Download:  PDF (310KB) ( )  
Export:  BibTeX | EndNote (RIS)      
An elite backcrossed inbred line Z550 with increased grains per panicle was identified from advanced backcrosses between Nipponbare and Xihui 18 by simple sequence repeat (SSR) marker-assisted selection (MAS).  Z550 carries 13 substitution segments distributed on chromosomes 1, 6, 7, 8, 9, 10, and 12, with an average substitution length of 1.68 Mb.  Compared with the Nipponbare parental line, plant height, panicle length, spikelets per panicle, grains per panicle, and grain weight for Z550 were significantly increased.  While the grain width of Z550 was significantly narrower, and the seed setting ratio (81.43%) was significantly lower than that of Nipponbare, it is still sufficient for breeding purposes.  Quantitative trait loci (QTLs) mapping for important agronomic traits was conducted with the F2 population derived from Nipponbare crossed with Z550 using the restricted maximum likelihood (REML) method.  A total of 16, including 12 previously unreported QTLs were detected, with contribution rates ranging from 1.46 to 10.49%.  Grains per panicle was controlled by 8 QTLs, 5 of which increased number of grains whereas 3 decreased it.  qGPP-1, with the largest contribution (10.49%), was estimated to increase grains per panicle by 30.67, while qGPP-9, with the minimum contribution rate (2.47%), had an effect of increasing grains per panicle by 15.79.  These results will be useful for further development of single segment substitution lines with major QTLs, and for research of their molecular functions via QTL cloning.
Keywords:  rice        backcrossed inbred line        yield        QTL mapping  
Received: 19 January 2018   Accepted:
Fund: The study was supported by the National Key R&D Program of China (2017YFD0100202), the Chongqing Science and Technology Commission Special Project, China (cstc2016shms-ztzx0017), and the Southwestern University Basic Operating Expenses Special Innovation Team Project, China (XDJK2017A004).
Corresponding Authors:  Correspondence ZHAO Fang-ming, Tel: +86-23-68250486, E-mail:   
About author:  WANG Shi-ming, E-mail:;

Cite this article: 

WANG Shi-ming, CUI Guo-qing, WANG Hui, MA Fu-ying, XIA Sai-sai, LI Yun-feng, YANG Zheng-lin, LING Ying-hua, ZHANG Chang-wei, HE Guang-hua, ZHAO Fang-ming. 2019. Identification and QTL mapping of Z550, a rice backcrossed inbred line with increased grains per panicle. Journal of Integrative Agriculture, 18(3): 526-531.

Abe Y, Mieda K, Ando T, Kono I, Yano M, Kitano H, Iwasaki Y. 2010. The small and round seed1 (SRS1/DEP2) gene is involved in the regulation of seed size in rice. Genes & Genetic Systems, 85, 327.
Ashikari M, Sakakibara H, Lin S Y, Yamamoto T, Takashi T, Nishimura A, Enrique R, Angeles Q Q, Kitano H, Matsuoka M. 2005. Cytokinin oxidase regulates rice grain production. Science, 309, 741–745.
Deshmukh R, Singh A, Jain N, Anand S, Gacche R, Singh A, Gaikwad K, Sharma T, Mohapatra T, Singh N. 2010. Identification of candidate genes for grain number in rice (Oryza sativa L.). Functional & Integrative Genomics, 10, 339–347.
Huang X, Qian Q, Liu Z, Sun H, He S Y, Luo D, Xia G M, Chu C C, Li J Y, Fu X D. 2009. Natural variation at the DEP1 locus enhances grain yield in rice. Nature Genetics, 41, 494.
Kobayashi K, Maekawa M, Miyao A, Hirochika H, Kyozuka J. 2010. PANICLE PHYTOMER2 (PAP2), encoding a SEPALLATA subfamily MADS-box protein, positively controls spikelet meristem identity in rice. Plant & Cell Physiology, 51, 47–57.
Li W, Wu J, Weng S, Zhang Y J, Zhang D P, Shi C H. 2010. Identification and characterization of dwarf 62, a loss-of-function mutation in DLT/OsGRAS-32 affecting gibberellin metabolism in rice. Planta, 232, 1383–1396.
Liu T, Mao D, Zhang S, Xu C, Xing Y. 2009. Fine mapping SPP1, a QTL controlling the number of spikelets per panicle, to a BAC clone in rice (Oryza sativa). Theoretical and Applied Genetics, 118, 1509–1517.
McCouch S R, Kochert G, Yu Z H. 1988. Molecular mapping of rice chromosome. Theoretical and Applied Genetics, 76, 148–159
Moralesramos Á I, Li Y H, Hilfiker M, Mecom J S, Eidam P, Shi D C, Tseng P, Brooks C, Zhang D, Wang N, Jaworski J, Morrow D, Fries H, Edwards R, Jin J. 2011. Structure-activity relationship studies of novel 3-oxazolidinedione-6-naphthyl-2-pyridinones as potent and orally bioavailable EP3 receptor antagonists. Bioorganic & Medicinal Chemistry Letters, 21, 2806–2811.
Paterson A H, Damon S, Hewitt J D, Zamir D, Rabinowitch H D, Lincoln S E, Lander E S, Tanksley S D. 1991. Mendelian factors underlying quantitative traits in tomato: Comparison across species, generations, and environments. Genetics, 127, 181–197.
Piao R, Jiang W Z, Ham T H, Choi M S, Qiao Y, Chu S H, Park J, Woo M O, Jin Z X, An G, Lee J Y, Kohet H J. 2009. Map-based cloning of the ERECT PANICLE 3 gene in rice. Theoretical and Applied Genetics, 119, 1497–506.
SAS Institute. 2009. SAS/STAT: Users’ guide. version 9.3.
Tan Y F, Li J X, Yu S B, Xu C G, Zhang Q F, Xing Y Z. 2000. Genetic bases of appearance quality of rice grains in Shanyou 63, an elite rice hybrid. Theoretical and Applied Genetics, 101, 823–829.
Xing Y Z, Tang W J, Xue W Y, Xu C G, Zhang Q. 2008. Fine mapping of a major quantitative trait loci, qSSP7, controlling the number of spikelets per panicle as a single mendelian factor in rice. Theoretical and Applied Genetics, 116, 789–796.
Xue W Y, Xing Y Z, Weng X Y, Zhao Y, Tang W J, Wang L, Zhou H J, Yu S B, Xu C G, Li X H, Zhang Q F. 2008. Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice. Nature Genetics, 40, 761–767.
Yu H, Murchie E H, Gonzálezcarranza Z H, Pyke K A, Roberts J A. 2015. Decreased photosynthesis in the erect panicle 3
(EP3) mutant of rice is associated with reduced stomatal conductance and attenuated guard cell development. Journal of Experimental Botany, 66, 1543.
Zhao F M, Tan Y, Zheng L Y, Zhou K, He G H, Ling Y H, Zhang L H, Xu S Z. 2016. Identification of rice chromosome segment substitution line Z322-1-10 and mapping QTLs for agronomic traits from the F3 population. Cereal Research Communication, 44, 1–11.
Zhou Y, Zhu J, Li Z, Yi C, Liu J, Zhang H, Tang S, Gu M, Liang G. 2009. Deletion in a quantitative trait gene qPE9-1 associated with panicle erectness improves plant architecture during rice domestication. Genetics, 183, 315.
Zhu K, Tang D, Yan C, Chi Z C, Yu H X, Chen J M, Liang J S, Gu M H, Cheng Z K. 2010. ERECT PANICLE2 encodes a novel protein that regulates panicle erectness in indica rice. Genetics, 184, 343.
[1] ZHAO Jun-yang, LU Hua-ming, QIN Shu-tao, PAN Peng, TANG Shi-de, CHEN Li-hong, WANG Xue-li, TANG Fang-yu, TAN Zheng-long, WEN Rong-hui, HE Bing. Soil conditioners improve Cd-contaminated farmland soil microbial communities to inhibit Cd accumulation in rice[J]. >Journal of Integrative Agriculture, 2023, 22(8): 2521-2535.
[2] GAO Peng, ZHANG Tuo, LEI Xing-yu, CUI Xin-wei, LU Yao-xiong, FAN Peng-fei, LONG Shi-ping, HUANG Jing, GAO Ju-sheng, ZHANG Zhen-hua, ZHANG Hui-min. Improvement of soil fertility and rice yield after long-term application of cow manure combined with inorganic fertilizers[J]. >Journal of Integrative Agriculture, 2023, 22(7): 2221-2232.
[3] SHI Shi-jie, ZHANG Gao-yu, CAO Cou-gui, JIANG Yang . Untargeted UHPLC–Q-Exactive-MS-based metabolomics reveals associations between pre- and post-cooked metabolites and the taste quality of geographical indication rice and regular rice[J]. >Journal of Integrative Agriculture, 2023, 22(7): 2271-2281.
[4] WEI Huan-he, GE Jia-lin, ZHANG Xu-bin, ZHU Wang, DENG Fei, REN Wan-jun, CHEN Ying-long, MENG Tian-yao, DAI Qi-gen. Decreased panicle N application alleviates the negative effects of shading on rice grain yield and grain quality[J]. >Journal of Integrative Agriculture, 2023, 22(7): 2041-2053.
[5] CHEN Guang-yi, PENG Li-gong, LI Cong-mei, TU Yun-biao, LAN Yan, WU Chao-yue, DUAN Qiang, ZHANG Qiu-qiu, YANG Hong, LI Tian. Effects of the potassium application rate on lipid synthesis and eating quality of two rice cultivars[J]. >Journal of Integrative Agriculture, 2023, 22(7): 2025-2040.
[6] LIU Yu, LIU Wen-wen, LI Li, Frederic FRANCIS, WANG Xi-feng. Transcriptome analysis reveals different response of resistant and susceptible rice varieties to rice stripe virus infection[J]. >Journal of Integrative Agriculture, 2023, 22(6): 1750-1762.
[7] DU Xiang-bei, XI Min, WEI Zhi, CHEN Xiao-fei, WU Wen-ge, KONG Ling-cong. Raised bed planting promotes grain number per spike in wheat grown after rice by improving spike differentiation and enhancing photosynthetic capacity[J]. >Journal of Integrative Agriculture, 2023, 22(6): 1631-1644.
[8] LI Min, ZHU Da-wei, JIANG Ming-jin, LUO De-qiang, JIANG Xue-hai, JI Guang-mei, LI Li-jiang, ZHOU Wei-jia. Dry matter production and panicle characteristics of high yield and good taste indica hybrid rice varieties[J]. >Journal of Integrative Agriculture, 2023, 22(5): 1338-1350.
[9] ZHANG Zi-han, NIE Jun, LIANG Hai, WEI Cui-lan, WANG Yun, LIAO Yu-lin, LU Yan-hong, ZHOU Guo-peng, GAO Song-juan, CAO Wei-dong. The effects of co-utilizing green manure and rice straw on soil aggregates and soil carbon stability in a paddy soil in southern China[J]. >Journal of Integrative Agriculture, 2023, 22(5): 1529-1545.
[10] CHEN Chang-zhao, WANG Ya-Liang, HE Meng-xing, LI Zhi-wen, SHEN Lan, LI Qing, RE De-yong, HU Jiang, ZHU Li, ZHANG Guang-heng, GAO Zhen-yu, ZENG Da-li, GUO Long-biao, QIAN Qian, ZHANG Qiang. OsPPR9 encodes a DYW-type PPR protein that affects editing efficiency of multiple RNA editing sites and is essential for chloroplast development[J]. >Journal of Integrative Agriculture, 2023, 22(4): 972-980.
[11] WANG Xin-yu, YANG Guo-dong, XU Le, XIANG Hong-shun, YANG Chen, WANG Fei, PENG Shao-bing. Grain yield and nitrogen use efficiency of an ultrashort-duration variety grown under different nitrogen and seeding rates in direct-seeded and double-season rice in Central China[J]. >Journal of Integrative Agriculture, 2023, 22(4): 1009-1020.
[12] WANG Yuan-zheng, Olusegun IDOWU, WANG Yun, HOMMA Koki, NAKAZAKI Tetsuya, ZHENG Wen-jing, XU Zheng-jin, SHIRAIWA Tatsuhiko.
Effects of erect panicle genotype and environment interactions on rice yield and yield components
[J]. >Journal of Integrative Agriculture, 2023, 22(3): 716-726.
[13] REN Chuan-ying, ZHANG Shan, HONG Bin, GUAN Li-jun, HUANG Wen-gong, FENG Jun-ran, SHA Di-xin, YUAN Di, LI Bo, JI Ni-na, LIU Wei, LU Shu-wen. Germinated brown rice relieves hyperlipidemia by alleviating gut microbiota dysbiosis[J]. >Journal of Integrative Agriculture, 2023, 22(3): 945-957.
[14] Kanokwan KAEWMUNGKUN, Keasinee TONGMARK, Sriprapai CHAKHONKAEN, Numphet SANGARWUT, Thiwawan WASINANON, Natjaree PANYAWUT, Khanittha DITTHAB, Kannika SIKAEWTUNG, QI Yong-bin, Sukanya DAPHA, Atikorn PANYA, Natthaporn PHONSATTA, Amorntip MUANGPROM. Development of new aromatic rice lines with high eating and cooking qualities[J]. >Journal of Integrative Agriculture, 2023, 22(3): 679-690.
[15] CAO Peng-hui, WANG Di, GAO Su, LIU Xi, QIAO Zhong-ying, XIE Yu-lin, DONG Ming-hui, DU Tan-xiao, ZHANG Xian, ZHANG Rui, JI Jian-hui. OsDXR interacts with OsMORF1 to regulate chloroplast development and the RNA editing of chloroplast genes in rice[J]. >Journal of Integrative Agriculture, 2023, 22(3): 669-678.
No Suggested Reading articles found!