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| Response of Nitrate Metabolism in Seedlings of Oilseed Rape (Brassica napus L.) to Low Oxygen Stress |
| YU Chang-bing, XIE Yu-yun, HOU Jia-jia, FU You-qiang, SHEN Hong , LIAO Xing |
1、Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture/Oil Crops Research Institute, Chinese Academy of
Agricultural Sciences, Wuhan 430062, P.R.China
2、College of Resources and Environment, South China Agricultural University, Guangzhou 510642, P.R.China |
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摘要 In order to understand the response of nitrate metabolism in seedlings of oilseed rape (Brassica napus L.) to low oxygen stress (LOS), two cultivars were studied at different light, LOS time and exogenous nitrate concentrations under hydroponic stress. Results show that N-uptake and dry matter of rape seedlings were decreased after LOS stress while nitrate accumulation (NA) under LOS was induced by darkness. Nitrate accumulation peaked at 3 d while root activity (RA, defined as dehydrogenase activity) decreased with prolonged waterlogging exposure. Exogenous nitrate significantly elevated NA and RA. Tungstate (TS) and LOS inhibited nitrate reductase (NR) activity while NR transcription and activity were enhanced by exogenous nitrate. Low oxygen stress stimulated the activity of superoxide dismutase (SOD) and peroxidase (POD) slightly, but inhibited that of catalase (CAT). B. napus L. Zhongshuang 10 (ZS10), a LOS tolerant cultivar, displayed smaller decrease upon dry matter under LOS, higher NA in darkness and lower NA in light than B. napus L. Ganlan CC (GAC), a LOS sensitive variety. ZS10 had lower NA and higher RA after waterlogging and exogenous nitrate treatment, and higher NR activity under TS inhibition than GAC, but the activity of antioxidant enzymes did not change under LOS. The results indicate that nitrate metabolism involved tolerance of rape seedlings to LOS, with lower accumulation and higher reduction of nitrate being related to higher LOS tolerance of rape seedlings exposed to waterlogging.
Abstract In order to understand the response of nitrate metabolism in seedlings of oilseed rape (Brassica napus L.) to low oxygen stress (LOS), two cultivars were studied at different light, LOS time and exogenous nitrate concentrations under hydroponic stress. Results show that N-uptake and dry matter of rape seedlings were decreased after LOS stress while nitrate accumulation (NA) under LOS was induced by darkness. Nitrate accumulation peaked at 3 d while root activity (RA, defined as dehydrogenase activity) decreased with prolonged waterlogging exposure. Exogenous nitrate significantly elevated NA and RA. Tungstate (TS) and LOS inhibited nitrate reductase (NR) activity while NR transcription and activity were enhanced by exogenous nitrate. Low oxygen stress stimulated the activity of superoxide dismutase (SOD) and peroxidase (POD) slightly, but inhibited that of catalase (CAT). B. napus L. Zhongshuang 10 (ZS10), a LOS tolerant cultivar, displayed smaller decrease upon dry matter under LOS, higher NA in darkness and lower NA in light than B. napus L. Ganlan CC (GAC), a LOS sensitive variety. ZS10 had lower NA and higher RA after waterlogging and exogenous nitrate treatment, and higher NR activity under TS inhibition than GAC, but the activity of antioxidant enzymes did not change under LOS. The results indicate that nitrate metabolism involved tolerance of rape seedlings to LOS, with lower accumulation and higher reduction of nitrate being related to higher LOS tolerance of rape seedlings exposed to waterlogging.
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Received: 12 October 2013
Accepted:
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| Fund: This research work is jointly supported by the Opening Foundation of Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, P.R.China (201010), and the Cultivation Project of Guangdong Province Institute of Higher Education Talents at High Levels and the Public Welfare Industry Special Scientific Research Funds (201203032). |
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Corresponding Authors:
SHEN Hong, Tel: +86-20-85281822, E-mail: 40592487@qq.com; LIAO Xing,
Tel: +86-27-86712256, E-mail: Liaox@oilcrops.cn
E-mail: 40592487@qq.com; Liaox@oilcrops.cn
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| About author: YU Chang-bing, Tel: +86-27-86712256, E-mail: cbyu123@163.com; |
Cite this article:
YU Chang-bing, XIE Yu-yun, HOU Jia-jia, FU You-qiang, SHEN Hong , LIAO Xing.
2014.
Response of Nitrate Metabolism in Seedlings of Oilseed Rape (Brassica napus L.) to Low Oxygen Stress. Journal of Integrative Agriculture, 13(11): 2416-2423.
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