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Journal of Integrative Agriculture  2024, Vol. 23 Issue (5): 1481-1493    DOI: 10.1016/j.jia.2023.06.020
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A Golden2-like transcription factor, BnGLK1a, improves chloroplast development, photosynthesis, and seed weight in rapeseed

Qianwei Zhang1, 2, 3*, Yuanyi Mao1, 2, 3*, Zikun Zhao1, 2, 3, Xin Hu2, 4, Ran Hu1, 2, 3, Nengwen Yin1, 2, 3, Xue Sun1, 2, 3, Fujun Sun1, 2, 3, Si Chen1, 2, 3, Yuxiang Jiang1, 2, 3, Liezhao Liu1, 2, 3, Kun Lu1, 2, 3, Jiana Li1, 2, 3#, Yu Pan2, 4#

1 Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Southwest University, Chongqing 400715, China

2 Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China

3 Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, China

4 Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education/College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China

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摘要  

提高光合效率是作物增产的重要要途径之一,叶绿体的合成和发育直接影响光合效率。本研究证明GOLDEN2-LIKE 1a (BnGLK1a在调控叶绿体发育与光合效率过程中发挥重要作用。超量表达BnGLK1a后,甘蓝型油菜中叶绿素含量、类囊体膜层数和光合效率均显著增加,而RNAi抑制BnGLK1a后产生结果则相反。通过酵母双杂交实验发现BnGLK1a与脱落酸受体BnPYL1-2BnCSN5A存在相互作用,可能一起参与调控叶绿体发育和光合作用,且BnGLK1a-RNAi株系对脱落酸处理表现敏感。更重要的是,与野生型相比,超量表达BnGLK1a后油菜千粒重增加10%,而抑制表达BnGLK1a后千粒重降低16%。因此,BnGLK1a作为提高油菜产量的重要潜基因靶位点。本研究不仅为揭示BnGLK1a功能机制提供线索,也为提高芸薹属作物产量提供了一条潜在途径。



Abstract  

Enhancing photosynthetic efficiency is a major goal for improving crop yields under agricultural field conditions and is associated with chloroplast biosynthesis and development.  In this study, we demonstrate that Golden2-like 1a (BnGLK1a) plays an important role in regulating chloroplast development and photosynthetic efficiency.  Overexpressing BnGLK1a resulted in significant increases in chlorophyll content, the number of thylakoid membrane layers and photosynthetic efficiency in Brassica napus, while knocking down BnGLK1a transcript levels through RNA interference (RNAi) had the opposite effects.  A yeast two-hybrid screen revealed that BnGLK1a interacts with the abscisic acid receptor PYRABACTIN RESISTANCE 1-LIKE 1–2 (BnPYL1–2) and CONSTITUTIVE PHOTOMORPHOGENIC 9 SIGNALOSOME 5A subunit (BnCSN5A), which play essential roles in regulating chloroplast development and photosynthesis.  Consistent with this, BnGLK1a-RNAi lines of B. napus display hypersensitivity to the abscisic acid (ABA) response.  Importantly, overexpression of BnGLK1a resulted in a 10% increase in thousand-seed weight, whereas seeds from BnGLK1a-RNAi lines were 16% lighter than wild type.  We propose that BnGLK1a could be a potential target in breeding for improving rapeseed productivity.  Our results not only provide insights into the mechanisms of BnGLK1a function, but also offer a potential approach for improving the productivity of Brassica species.

Keywords:  Brassica napus        BnGLK1a        chloroplast development        photosynthetic efficiency        yield   
Received: 23 February 2023   Accepted: 07 June 2023
Fund: 

This work was funded by the National Natural Science Foundation of China (32172597 and 31830067), the Chongqing Talents of Exceptional Young Talents Project, China (CQYC202005097, cstc2021ycjh-bgzxm0204, and cstc2021jcyj-bshX0002), the China Agriculture Research System of MOF and MARA (CARS-12), the 111 Project, China (B12006), and the Germplasm Creation Special Program of Southwest University, China.

About author:  #Correspondence Jiana Li, Tel: +86-23-68251264, E-mail: ljn1950@swu.edu.cn; Yu Pan, Tel: +86-23-68250227, E-mail: panyu1020@swu.edu.cn * These authors contributed equally to this study.

Cite this article: 

Qianwei Zhang, Yuanyi Mao, Zikun Zhao, Xin Hu, Ran Hu, Nengwen Yin, Xue Sun, Fujun Sun, Si Chen, Yuxiang Jiang, Liezhao Liu, Kun Lu, Jiana Li, Yu Pan. 2024.

A Golden2-like transcription factor, BnGLK1a, improves chloroplast development, photosynthesis, and seed weight in rapeseed . Journal of Integrative Agriculture, 23(5): 1481-1493.

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