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Journal of Integrative Agriculture  2023, Vol. 22 Issue (4): 1045-1057    DOI: 10.1016/j.jia.2023.01.005
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Optimization of slow-release fertilizer application improves lotus rhizome quality by affecting the physicochemical properties of starch

ZHAO Shu-ping1*, DENG Kang-ming1*, ZHU Ya-mei1, JIANG Tao1, WU Peng1, FENG Kai1, LI Liang-jun1, 2#

1 College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, P.R.China

2 Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, P.R.China

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摘要  为了实现高产优质、环境成本低的双重目标,缓释肥作为新型肥料取代传统氮肥被广泛应用于莲藕栽培。然而,优化的缓释肥施用量如何改善莲藕品质的机制尚不清楚。本研究旨在研究6个缓释肥水平(CK、S1、S2、S3、S4、S5)下对莲藕的光合特性和淀粉合成、积累和理化性质的影响。与CK(0 kg∙ha-1 SRF)相比,缓释肥处理下莲藕叶片的Pn和SPAD值保持较高水平。进一步研究表明,缓释肥提高了莲藕产量、直链淀粉、支链淀粉、总淀粉含量和淀粉粒数量。在6个缓释肥水平中,S3(1035 kg∙ha-1 SRF)与CK相比差异最大,并保持在最高水平。随着缓释肥水平的升高,峰值、热浆和最终粘度先降低,后增加,但消减值和糊化温度升高。为了在分子水平上解释这一变化,我们分析了淀粉积累相关酶的活性和淀粉积累相关酶基因的相对表达量,结果表明缓释肥处理下均增加,特别是S3处理下增加最多。总体来说,缓释肥,特别是S3(1035kg∙ha-1 SRF)作为莲藕种植的适宜肥料比,可以通过影响淀粉积累相关酶和基因来提高莲藕品质。这些结果将有助于低环境成本的优质莲藕的生产。


To achieve the dual goals of high yield and good quality with low environmental costs, slow-release fertilizer (SRF) has been widely used in lotus cultivation as new type of fertilizer instead of traditional nitrogen fertilizer.  However, the optimal amount of SRF and how it would promote lotus rhizome quality remain unclear.  This study was designed to investigate the photosynthetic characteristics and the synthesis, accumulation, and physicochemical properties of lotus rhizome starches under six SRF levels (CK, S1, S2, S3, S4, and S5).  Compared with CK (0 kg ha–1), the net photosynthetic rate (Pn) and SPAD values of leaves remained at higher levels under SRF treatment.  Further research showed that SRF increased the lotus rhizome yield, the contents of amylose, amylopectin, and total starch, and the number of starch granules.  Among the six SRF levels, S3 (1 035 kg ha–1) showed the greatest difference from CK and produced the highest levels.  With the increasing SRF levels, the peak, hot and final viscosities decreased at first and then increased, but the setback viscosity and pasting temperature increased.  In order to interpret these changes at the molecular level, the activities of key enzymes and relative expression levels of starch accumulation related genes were analyzed.  Each of these parameters also increased under SRF treatment, especially under the S3 treatment.  The results of this study show that SRF, especially S3 (1 035 kg ha–1), is a suitable fertilizer option for lotus planting which can improve lotus rhizome quality by affecting starch accumulations related enzymes and genes.  These results will be useful for SRF application to high-quality lotus rhizome production with low environmental costs.

Keywords:  Nelumbo nucifera Gaertn.       slow-release fertilizer (SRF)        lotus rhizome yield        starch        gene expression  
Received: 28 May 2022   Accepted: 14 November 2022
Fund: The authors are grateful for the financial support they received from the National Key R&D Program of China (2020YFD1000300), the earmarked fund for China Agriculture Research System (CARS-24), and the High-Level Talent Support Plan (Lv-Yang-Jin-Feng), Yangzhou, China.

About author:  #Correspondence LI Liang-jun, E-mail: * These authors contributed equally to this study.

Cite this article: 

ZHAO Shu-ping, DENG Kang-ming, ZHU Ya-mei, JIANG Tao, WU Peng, FENG Kai, LI Liang-jun. 2023.

Optimization of slow-release fertilizer application improves lotus rhizome quality by affecting the physicochemical properties of starch . Journal of Integrative Agriculture, 22(4): 1045-1057.

Bao J, Kong X, Xie J, Xu L. 2004. Analysis of genotypic and environmental effects on rice starch. 1. Apparent amylose content, pasting viscosity, and gel texture. Journal of Agricultural and Food Chemistry, 52, 6010–6016.
Beig B, Niazi M B K, Jahan Z, Hussain A, Zia M H, Mehran M T. 2020. Coating materials for slow release of nitrogen from urea fertilizer: A review. Journal of Plant Nutrition, 43, 1510–1533.
Chen G X, Zhu J T, Zhou, J W, Subburaj S, Zhang M, Han C X, Hao P C, Li X H, Yan Y M. 2014. Dynamic development of starch granules and the regulation of starch biosynthesis in Brachypodium distachyon: Comparison with common wheat and Aegilops peregrina. BMC Plant Biology, 14, 198.
Chen Y, Pang Y, Bao J. 2020. Expression profiles and protein complexes of starch biosynthetic enzymes from white-core and waxy mutants induced from high amylose indica rice. Rice Science, 27, 152–161.
Chen Y L, Tu P F, Yang Y B, Xue X H, Feng Z H, Dan C X, Cheng F X, Yang Y F, Deng L S. 2022. Diversity of rice rhizosphere microorganisms under different fertilization modes of slow-release fertilizer. Scientific Reports, 12, 2694.
Dong Y J, He M R, Wang Z L, Chen W F, Hou J, Qiu X K, Zhang J W. 2016. Effects of new coated release fertilizer on the growth of maize. Journal of Soil Science and Plant Nutrition, 16, 637–649.
Elghareeb E M, El-Shahaby O A, Abo-Hamed S A, Ibraheem F. 2021. Metabolic responses to contrasting levels of N fertilization during ear leaf transition from assimilation to remobilization at grain filling in maize. Egyptian Journal of Botany, 61, 637–654.
Gani A, Masoodi F A, Wani S M. 2013. Characterization of lotus stem (Nelumbo nucifera) starches purified from three lakes of India. Journal of Aquatic Food Product Technology, 22, 605–618.
Gao L C, Xia M J, Wan C X, Jia Y H, Yang L M, Wang M, Wang P K, Yang Q H, Yang P, Gao X L, Gao J F. 2021. Analysis of synthesis, accumulation and physicochemical properties of Tartary buckwheat starches affected by nitrogen fertilizer. Carbohydrate Polymers, 273, 118570.
Gil-Ortiz R, Naranjo M A, Ruiz-Navarro A, Atares S, Garcia C, Zotarelli L, San Bautista A, Vicente O. 2020. Enhanced agronomic efficiency using a new controlled-released, polymeric-coated nitrogen fertilizer in rice. Plants (Basel), 9, 1183.
Gu J F, Chen J, Chen L, Wang Z Q, Zhang H, Yang J C. 2015. Grain quality changes and responses to nitrogen fertilizer of japonica rice cultivars released in the Yangtze River Basin from the 1950s to 2000s. Crop Journal, 3, 285–297.
Guan W, Shao X, Li Y. 2020. Effects of irrigation and nitrogen management on the growth and nitrogen concentration of paddy soil and rice plants. Polish Journal of Environmental Studies, 29, 4053–4063.
Guo H B. 2009. Cultivation of lotus (Nelumbo nucifera Gaertn. ssp. nucifera) and its utilization in China. Genetic Resources and Crop Evolution, 56, 323–330.
Han H X, Yang C T, Zhu J H, Zhang L X, Bai Y M, Li E P, Gilbert R G. 2019. Competition between granule bound starch synthase and starch branching enzyme in starch biosynthesis. Rice, 12, 96.
Iqbal A, Xie H M, He L, Ahmad S, Hussain I, Raza H, Khan A, Wei S Q, Quan Z, Wu K, Ali I, Jiang L G. 2021. Partial substitution of organic nitrogen with synthetic nitrogen enhances rice yield, grain starch metabolism and related genes expression under the dual cropping system. Saudi Journal of Biological Sciences, 28, 1283–1296.
Ishimaru T, Hirose T, Matsuda T, Goto A, Takahashi K, Sasaki H, Terao T, Ishii R, Ohsugi R, Yamagishi T. 2005. Expression patterns of genes encoding carbohydrate-metabolizing enzymes and their relationship to grain filling in rice (Oryza sativa L.): Comparison of caryopses located at different positions in a panicle. Plant and Cell Physiology, 46, 620–628.
Kenawy E R, Hosny A, Saad-Allah K. 2021. Reducing nitrogen leaching while enhancing growth, yield performance and physiological traits of rice by the application of controlled-release urea fertilizer. Paddy and Water Environment, 19, 173–188.
Kiba T, Krapp A. 2016. Plant nitrogen acquisition under low availability: Regulation of uptake and root architecture. Plant and Cell Physiology, 57, 707–714.
Kong X, Zhu P, Sui Z, Bao J. 2015. Physicochemical properties of starches from diverse rice cultivars varying in apparent amylose content and gelatinisation temperature combinations. Food Chemistry, 172, 433–440.
Kumaraswamy R V, Saharan V, Kumari S, Chandra R C, Pal A, Sharma S S, Rakshit S, Raliya R, Biswas P. 2021. Chitosan-silicon nanofertilizer to enhance plant growth and yield in maize (Zea mays L.). Plant Physiology and Biochemistry, 159, 53–66.
Lawrencia D, Wong S K, Low D Y S, Goh B H, Goh J K, Ruktanonchai U R, Soottitantawat A, Lee L H, Tang S Y. 2021. Controlled release fertilizers: A review on coating materials and mechanism of release. Plants (Basel), 10, 238.
Li D B, Lyu G Z, Jiang Y M, Niu H B, Wang X, Yin J. 2019. Effects of exogenous RSRHA2B gene on key enzyme activities and expression of related genes in the grain filling stage of wheat (Triticum aestivum L.). Applied Ecology and Environmental Research, 17, 15073–15086.
Li T, Chen X G, Xu W H, Chi S L, Zhao W Y, Li Y H, Zhang C L, Feng D Y, He Z M, Wang Z Y. 2018. Effects of coated slow-release fertilizer with urease and nitrification inhibitors on nitrogen release characteristic and uptake and utilization of nitrogen, phosphorus and potassium in cabbage. International Journal of Agriculture and Biology, 20, 422–430.
Liu T Q, Huang J F, Chai K B, Cao C G, Li C F. 2018. Effects of N fertilizer sources and tillage practices on NH3 volatilization, grain yield, and N use efficiency of rice fields in central China. Frontiers in Plant Science, 9, 385.
Liu X M, Gu W R, Li C F, Li J, Wei S. 2021. Effects of nitrogen fertilizer and chemical regulation on spring maize lodging characteristics, grain filling and yield formation under high planting density in Heilongjiang Province, China. Journal of Integrative Agriculture, 20, 511–526.
Liu Y, Sun D, Wang H, Wang X, Yu G, Zhao X. 2020. An evaluation of China’s agricultural green production: 1978–2017. Journal of Cleaner Production, 243, 118483.
Livak K. Schmittgen T. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−∆∆CT method. Methods, 25, 402–408.
Man J M, Cai J W, Cai C H, Xu B, Huai H Y, Wei C X. 2012. Comparison of physicochemical properties of starches from seed and rhizome of lotus. Carbohydrate Polymers, 88, 676–683.
Mu X, Chen Y. 2021. The physiological response of photosynthesis to nitrogen deficiency. Plant Physiology and Biochemistry, 158, 76–82.
Mu X H, Chen Q W, Chen F J, Yuan L X, Mi G H. 2017. A RNA-Seq analysis of the response of photosynthetic system to low nitrogen supply in maize leaf. International Journal of Molecular Sciences, 18, 2624.
Naz M Y, Sulaiman S A. 2016. Slow release coating remedy for nitrogen loss from conventional urea: A review. Journal of Controlled Release, 225, 109–120.
Patindol J A, Siebenmorgen T J, Wang Y J. 2015. Impact of environmental factors on rice starch structure: A review. Starch-Stärke, 67, 42–54.
Ran L P, Yu X R, Li Y Q, Zou J C, Deng J W, Pan J Y, Xiong F. 2020. Analysis of development, accumulation and structural characteristics of starch granule in wheat grain under nitrogen application. International Journal of Biological Macromolecules, 164, 3739–3750.
Ren X J, Ci J B, Jiang L Y, Yang W G, Yang W. 2018. Effects of physicochemical properties of different substrates on characters and yield of maize. Emirates Journal of Food and Agriculture, 30, 851–862.
Simi C K, Abraham T E. 2008. Physicochemical rheological and thermal properties of Njavara rice (Oryza sativa L.) starch. Journal of Agricultural and Food Chemistry, 56, 12105–12113.
Vejan P, Khadiran T, Abdullah R, Ahmad N. 2021. Controlled release fertilizer: A review on developments, applications and potential in agriculture. Journal of Controlled Release, 339, 321–334.
Wang J, Wen Z, Fu P, Lu W, Lu D. 2019. Effects of nitrogen rates on the physicochemical properties of waxy maize starch. Starch-Stärke, 71, 1900146.
Wani A A, Singh P, Shah M A, Schweiggert-Weisz U, Gul K, Wani I A. 2012. Rice starch diversity: effects on structural, morphological, thermal, and physicochemical properties - A review. Comprehensive Reviews in Food Science and Food Safety, 11, 417–436.
Wei H Y, Chen Z F, Xing Z P, Zhou L, Liu Q Y, Zhang Z Z, Jiang Y, Hu Y J, Zhu J Y, Cui P Y, Dai Q G, Zhang H C. 2018. Effects of slow or controlled release fertilizer types and fertilization modes on yield and quality of rice. Journal of Integrative Agriculture, 17, 2222–2234.
Wang W T, Cui W P, Xu K, Gao H, Wei H Y, Zhang H C. 2021. Effects of early- and late-sowing on starch accumulation and associated enzyme activities during grain filling stage in rice. Rice Science, 28, 191–199.
Wu P, Zhang Y Y, Zhao S P, Li L J. 2021. Comprehensive analysis of evolutionary characterization and expression for monosaccharide transporter family genes in Nelumbo nucifera. Frontiers in Ecology and Evolution, 9, 537398.
Wu Q, Wang Y H, Ding Y F, Tao W K, Gao S, Li Q X, Li W W, Liu Z H, Li G H. 2021. Effects of different types of slow- and controlled-release fertilizers on rice yield. Journal of Integrative Agriculture, 20, 1503–1514.
Wu Q, Wang Y Z, Chen T T, Zheng J L, Sun Y D, Chi D C. 2020. Soil nitrogen regulation using clinoptilolite for grain filling and grain quality improvements in rice. Soil & Tillage Research, 199, 104547.
Xu D, Zhu Y, Zhu H B, Hu Q, Liu G D, Wei H Y, Zhang H C. 2021. Effects of a one-time application of controlled-release nitrogen fertilizer on yield and nitrogen accumulation and utilization of late japonica rice in China. Agriculture (Basel), 11, 1041.
Yamakawa H, Hirose T, Kuroda M, Yamaguchi T. 2007. Comprehensive expression profiling of rice grain filling-related genes under high temperature using DNA microarray. Plant Physiology, 144, 258–277.
Yang X Y, Bi J G, Gilbert R G, Li G H, Liu Z H, Wang S H, Ding Y F. 2016. Amylopectin chain length distribution in grains of japonica rice as affected by nitrogen fertilizer and genotype. Journal of Cereal Science, 71, 230–238.
Yang Y, Liu B, Ni X, Tao L, Yu L, Yang Y, Feng M, Zhong W, Wu Y. 2021. Rice productivity and profitability with slow-release urea containing organic-inorganic matrix materials. Pedosphere, 31, 511–520.
Yu H, Cheng L, Yin J, Yan S, Liu K, Zhang F, Xu B, Li L. 2013. Structure and physicochemical properties of starches in lotus (Nelumbo nucifera Gaertn.) rhizome. Food Science & Nutrition, 1, 273–283.
Yuan C, Wang S Y, Lu D L. 2022. Fertilization time of slow-release fertilizer affects the physicochemical properties of starch from spring-sown waxy maize. Journal of the Science of Food and Agriculture, 102, 1012–1020.
Zhang C H, Jiang D, Liu F L, Cai J, Dai T B, Cao W X. 2010. Starch granules size distribution in superior and inferior grains of wheat is related to enzyme activities and their gene expressions during grain filling. Journal of Cereal Science, 51, 226–233.
Zhang W L, Yang Q H, Xia M J, Bai W M, Wang P K, Gao X L, Li J , Feng B L, Gao J F. 2019. Effects of nitrogen level on the physicochemical properties of Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) starch. International Journal of Biological Macromolecules, 129, 799–808.
Zhao B, Dong S T, Zhang J W, Liu P. 2013. Effects of controlled-release fertiliser on nitrogen use efficiency in summer maize. PLoS ONE, 8, e70569.
Zhao S, Ruan F, Shen W, Deng K, Jiang T, Wu P, Feng K, Li L. 2022. The effect of nitrogen fertilizer on rhizome quality and starch physicochemical properties in Nelumbo nucifera. Agronomy (Basel), 12, 794.
Zheng Y, Han X, Li Y, Liu S, Ji J, Tong Y. 2020. Effects of mixed controlled release nitrogen fertilizer with rice straw biochar on rice yield and nitrogen balance in Northeast China. Scientific Reports, 10, 9452.
Zhou T Y, Zhou Q, Li E P, Yuan L M, Wang W L, Zhang H, Liu L J, Wang Z Q, Yang J C, Gu J F. 2020. Effects of nitrogen fertilizer on structure and physicochemical properties of ‘super’ rice starch. Carbohydrate Polymers, 239, 116237.
Zhou W, Lyu T F, Yang Z P, Sun H, Yang L J, Chen Y, Ren W J. 2016. Research advances on regulating soil nitrogen loss by the type of nitrogen fertilizer and its application strategy. Chinese Journal of Applied Ecology, 27, 3051–3058. (in Chinese)
Zi Y, Ding J F, Song J M, Humphreys G, Peng Y X, Li C Y, Zhu X K, Guo W S. 2018. Grain yield, starch content and activities of key enzymes of waxy and non-waxy wheat (Triticum aestivum L.). Scientific Reports, 8, 4548.

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