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| Study on Hsp90 Expression in Different Tissues and Its Antibody in Serum of Chickens Infected with Marek’s Diseases |
| LI Yu-bao, LI Juan, WANG Zhi-liang , LIU Si-dang |
1、School of Agriculture, Liaocheng University, Liaocheng 252059, P.R.China
2、School of Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, P.R.China
3、China Animal Health and Epidemiology Center, Qingdao 266032, P.R.China |
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摘要 To investigate the dynamic change of heat shock protein 90 (Hsp90) in the genesis and development of tumor, we successfully established tumor animal model using Marek’s disease and then determined the location of Hsp90 in the tumor tissue using immunohistochemistry method, the antibody titer level of Hsp90 in the serum and the expression level in the tissue using enzymelinked immunosorbent assay (ELISA) method. Our result showed that Hsp90 location in the tumor tissue was significantly associated with the tumor cell and most in the cytoplasm of the tumor cell, and Hsp90 expression level in the tissue and the antibody titer level in the serum was most significantly increased with the development of tumor. This is the first report to show the presence of Hsp90 in tumor tissues induced by the Marek’s disease, with its expression correlated to the tumoral grading. These data may also be valuable for developing new molecular anti-cancer therapies.
Abstract To investigate the dynamic change of heat shock protein 90 (Hsp90) in the genesis and development of tumor, we successfully established tumor animal model using Marek’s disease and then determined the location of Hsp90 in the tumor tissue using immunohistochemistry method, the antibody titer level of Hsp90 in the serum and the expression level in the tissue using enzymelinked immunosorbent assay (ELISA) method. Our result showed that Hsp90 location in the tumor tissue was significantly associated with the tumor cell and most in the cytoplasm of the tumor cell, and Hsp90 expression level in the tissue and the antibody titer level in the serum was most significantly increased with the development of tumor. This is the first report to show the presence of Hsp90 in tumor tissues induced by the Marek’s disease, with its expression correlated to the tumoral grading. These data may also be valuable for developing new molecular anti-cancer therapies.
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Received: 21 February 2013
Accepted:
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| Fund: This work was supported by the National Natural Science Foundation of China (31101787) and the Natural Science Foundation of Shandong Province, China (ZR2010CM035). |
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Corresponding Authors:
WANG Zhi-liang, Tel/Fax: +86-532-87839188, E-mail: zlwang111@163.com; LIU Si-dang, Tel/Fax: +86-538-8249526, E-mail: liusidang@126.com
E-mail: zlwang111@163.com;liusidang@126.com
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| About author: LI Yu-bao, E-mail: liyubao@lcu.edu.cn; LI Juan, E-mail: li198820062011@163.com |
Cite this article:
LI Yu-bao, LI Juan, WANG Zhi-liang , LIU Si-dang.
2014.
Study on Hsp90 Expression in Different Tissues and Its Antibody in Serum of Chickens Infected with Marek’s Diseases. Journal of Integrative Agriculture, 13(6): 1355-1362.
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