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| Transformation of Cabbage (Brassica oleracea L. var. capitata) with Bt cry1Ba3 Gene for Control of Diamondback Moth |
| YI Deng-xia, CUI Lei, LIU Yu-mei, ZHUANG Mu, ZHANG Yang-yong, FANG Zhi-yuan , YANG Li-mei |
| 1.Key Laboratory of Horticultural Crops Genetic Improvement, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences |
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摘要 To obtain transgenic cabbage line with broad insect resistance, a new synthetic Bacillus thuringiensis cry1Ba3 gene was introduced into white cabbage via Agrobacterium tumefaciens-mediated transformation and 37 transformants were obtained. Polymerase chain reaction (PCR) and Southern blot analyses confirmed that cry1Ba3 was successfully inserted into the genome of cabbage. Reverse transcription-polymerase chain reaction (RT-PCR) demonstrated that cry1Ba3 was expressed. Western blot results confirmed the production of insecticidal protein encoded by cry1Ba3. Insect bioassays showed that transgenic cabbages effectively controlled both susceptible and Cry1Ac-resistant diamondback moth (DBM) larvae.
Abstract To obtain transgenic cabbage line with broad insect resistance, a new synthetic Bacillus thuringiensis cry1Ba3 gene was introduced into white cabbage via Agrobacterium tumefaciens-mediated transformation and 37 transformants were obtained. Polymerase chain reaction (PCR) and Southern blot analyses confirmed that cry1Ba3 was successfully inserted into the genome of cabbage. Reverse transcription-polymerase chain reaction (RT-PCR) demonstrated that cry1Ba3 was expressed. Western blot results confirmed the production of insecticidal protein encoded by cry1Ba3. Insect bioassays showed that transgenic cabbages effectively controlled both susceptible and Cry1Ac-resistant diamondback moth (DBM) larvae.
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Received: 30 January 2011
Accepted:
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| Fund: This work was supported by the grants from the National High Technology Research and Development Program of China (863 Program, 2008AA10Z155), the National Natural Science Foundation of China (31071697), and the earmarked fund for the Modern Agro-Industry Technology Research System, China (nycytx-35-gw01). |
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Corresponding Authors:
Correspondence YANG Li-mei, Tel: +86-10-82108756, Fax: +86-10-62174123, E-mail: yanglm@mail.caas.net.cn
E-mail: yanglm@mail.caas.net.cn
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Cite this article:
YI Deng-xia, CUI Lei, LIU Yu-mei, ZHUANG Mu, ZHANG Yang-yong, FANG Zhi-yuan , YANG Li-mei.
2011.
Transformation of Cabbage (Brassica oleracea L. var. capitata) with Bt cry1Ba3 Gene for Control of Diamondback Moth . Journal of Integrative Agriculture, 10(11): 1693-1700.
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