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Colonization by Klebsiella variicola FH-1 stimulates soybean growth and alleviates the stress of Sclerotinia sclerotiorum
ZHAI Qian-hang1*, PAN Ze-qun1*, ZHANG Cheng1, YU Hui-lin2, ZHANG Meng1, GU Xue-hu1, ZHANG Xiang-hui2, PAN Hong-yu2, ZHANG Hao1

1 College of Plant Protection, Jilin Agricultural University, Changchun 130118, P.R.China 2 College of Plant Sciences, Jilin University, Changchun 130062, P.R.China

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摘要  由核盘菌(Sclerotinia sclerotiorum)引起的菌核病是一种毁灭性的土传大豆病害,会造成巨大的产量损失。我们以前报道过变栖克雷伯氏菌FH-1可以降解莠去津除草剂,并且可以增加莠去津敏感作物(如大豆)的营养生长。我们发现FH-1可以促进大豆生长并诱导对菌核病菌的抗性,为了证明FH-1对大豆菌核病菌的生防机制并评价其生防能力,我们在体外培养基试验中证明菌株FH-1可以固定培养基中的氮,溶解无机磷和钾,并产生吲哚乙酸和铁载体,具有促进植物生长的潜力。盆栽试验结果表明,变栖克雷伯氏菌FH-1能促进大豆植株发育,显著提高株高、鲜重和根长,并诱导大豆叶片对菌核病的抗性。用菌株FH-1治疗的疾病进展曲线下面积(AUDPC)显著低于对照,并且在48小时内减少了高达42.2%。(P < 0.001)。此外,紫外分光光度计法测量结果表明菌株FH-1可以增强参与大豆植物防御的过氧化氢酶、超氧化物歧化酶、过氧化物酶、苯丙氨酸解氨酶和多酚氧化酶的酶活性,并减少了叶片中丙二醛的积累。定量实时PCR检测了可能参与大豆抵抗核盘菌胁迫的相关基因的转录水平,结果表明诱导抗性的机制似乎主要是由于变栖克雷伯氏菌FH-1诱导PR10、PR12、AOS、CHS和PDF1.2基因转录水平的提高。利用结晶紫染色法测定了菌株FH-1具有生物膜形成能力,用共聚焦荧光显微镜和扫描电镜观测菌株FH-1在大豆根上的定殖情况,菌株FH-1可以定殖在大豆根表面、根毛和外皮层上形成生物膜。综上所述,变栖克雷伯氏菌FH-1在大豆根部的定殖有助于诱导参与植物保护的防御酶和相关防御基因的表达,诱导大豆对菌核病菌的抗性表现出生物防治潜力。这对大豆的种植和生长具有重要意义。此外,本研究有助于理解变种芽胞杆菌FH-1、大豆植株和核盘菌之间相互作用的有价值的第一步,这为绿色防控提供了新的思路。

Abstract  Sclerotinia stem rot, caused by Sclerotinia sclerotiorum, is a destructive soil-borne disease of Glycine max (L.) Merr that leads to huge yield loss. We previously reported that Klebsiella variicola FH-1 could degrade atrazine herbicides, and the vegetative growth of atrazine-sensitive crops (i.e., soybean) was significantly increased in the FH-1-treated soil. Interestingly, we found that FH-1 could promote soybean growth and induce resistance to S. sclerotiorum. In our study, strain FH-1 could grow in a nitrogen-free environment, dissolve inorganic phosphorus and potassium, and produce indoleacetic acid and a siderophore. The results of pot experiments showed that K. variicola FH-1 promoted soybean plant development, substantially improving plant height, fresh weight, and root length, and induced resistance against S. sclerotiorum infection in soybean leaves. The area under the disease progression curve (AUDPC) for treatment with strain FH-1 was significantly lower than the control and was reduced by up to 42.2% within 48 hours (P<0.001). Moreover, strain FH-1 rcovered the activities of catalase, superoxide dismutase, peroxidase, phenylalanine ammonia lyase, and polyphenol oxidase, which are involved in plant protection, and reduced malondialdehyde accumulation in the leaves. The mechanism of induction of resistance appeared to be primarily resulted from the enhancement of transcript levels of PR10, PR12, AOS, CHS, and PDF1.2 genes. The colonization of FH-1 on soybean root, determined using CLSM and SEM, revealed that FH-1 colonized soybean root surfaces, root hairs, and exodermis to form biofilms. In summary, K. variicola FH-1 exhibited the biological control potential by inducing resistance in soybean against S. sclerotiorum infection, and this provides new suggestions for green prevention and control.
Keywords:  sclerotinia stem rot of soybean       Klebsiella variicola FH-1        inducing resistance        root colonization        biofilm  
Online: 24 January 2023  

This work was financially supported by grants from the Inter-governmental International Cooperation Special Project of National Key & D Program of China (2019YFE0114200), and the Natural Science Foundation Project of Science and Technology Department of Jilin Province, China (20200201215JC).

About author:  ZHAI Qian-hang, E-mail:; PAN Ze-qun, E-mail:; Correspondence ZHANG Hao, E-mail: * These authors contributed equally to this work.

Cite this article: 

ZHAI Qian-hang, PAN Ze-qun, ZHANG Cheng, YU Hui-lin, ZHANG Meng, GU Xue-hu, ZHANG Xiang-hui, PAN Hong-yu, ZHANG Hao. 2023. Colonization by Klebsiella variicola FH-1 stimulates soybean growth and alleviates the stress of Sclerotinia sclerotiorum. Journal of Integrative Agriculture, Doi:10.1016/j.jia.2023.01.007

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