Lack of fitness cost and inheritance of resistance to abamectin based on the establishment of a near-isogenic strain of Tetranychus urticae

doi:10.1016/j.jia.2022.10.012

Journal of Integrative Agriculture

2023, Vol. 22

Issue (6): 1809-1819 DOI: 10.1016/j.jia.2022.10.012

Plant Protection

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Lack of fitness cost and inheritance of resistance to abamectin based on the establishment of a near-isogenic strain of Tetranychus urticae

ZHANG Yan^1*, TIAN Tian^{1, 2*}, ZHANG Kun³, ZHANG You-jun¹, WU Qing-jun¹, XIE Wen¹, GUO Zhao-jiang¹, WANG Shao-li^1#

¹State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

²College of Agriculture, Yangtze University, Jingzhou 434025, P.R.China

³Sanya Nanfan Research Institute, Hainan University, Sanya 572024, P.R.China

Abstract
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摘要

二斑叶螨Tetranychus urticae Koch对阿维菌素的高水平抗性在中国及其他国家的田间种群中普遍存在。为阐明中国二斑叶螨田间种群对阿维菌素抗性的遗传模式、交互抗性和适合度代价，本研究将采集到的田间抗性种群经过多代与室内敏感品系IPP-SS的回交、孤雌生殖和阿维菌素筛选等过程，最终将田间二斑叶螨对阿维菌素高抗性的特征导入到敏感品系IPP-SS中，构建了一个抗阿维菌素的近等基因系NIL-Aba。与IPP-SS品系相比，NIL-Aba品系对阿维菌素的抗性为25,147倍，对联苯菊酯呈现高水平的交互抗性（288.17倍），对甲维盐为中等水平的交互抗性（42.57倍），对联苯肼酯、虫螨腈、丁氟螨酯、腈吡螨酯和乙唑螨腈为3.18-9.31倍的低交互抗性，对丙溴磷无交互抗性。NIL-Aba品系对阿维菌素抗性的遗传模式为常染色体、不完全显性遗传，且受多基因控制。基于两性生命表参数分析，NIL-Aba品系不存在适合度代价。建立二斑叶螨NIL-Aba品系可为后续对阿维菌素抗性的深入研究提供可靠基础，毒理学参数及适合度代价相关数据将有助于二斑叶螨田间种群的阿维菌素抗性治理。

Abstract

Many populations of the two-spotted spider mite, Tetranychus urticae Koch, have developed high levels of resistance to the pesticide abamectin in China and other countries. This study developed a near-isogenic line to understand better the inheritance, cross-resistance, and fitness costs associated with abamectin resistance in the field population of T. urticae in China. We introduced the trait that confers extremely high abamectin resistance in a field-collected population of T. urticae into a susceptible laboratory strain (IPP-SS) to generate an abamectin-resistant near-isogenic line (NIL-Aba). This process was carried out through multiple backcrossing to IPP-SS and via parthenogenesis and abamectin screening. Compared with IPP-SS, the NIL-Aba strain had a 25 147-fold resistance to abamectin and a high level of cross-resistance to bifenthrin (288.17-fold), an intermediate level to emamectin benzoate (42.57-fold), and low levels to bifenazate, chlorfenapyr, cyflumetofen, cyenopyrafen, and cyetpyrafen with resistance ranging from 3.18- to 9.31-fold. But it had no cross-resistance to profenofos. The resistance to abamectin in NIL-Aba was autosomal, incompletely dominant, and polygenic. Based on two sex life table parameters, no fitness cost was found in NIL-Aba. Establishing the NIL-Aba strain provides a reliable basis for an in-depth study of abamectin resistance in T. urticae. New information on toxicological characteristics and fitness cost should facilitate the management of abamectin resistance in field populations of T. urticae.

Keywords: two-spotted spider mite abamectin resistance near-isogenic strain inheritance pattern fitness cost

Received: 16 July 2022 Online: 13 October 2022 Accepted: 31 August 2022

Fund:

This research was funded by the National Natural Science Foundation of China (32072458), the earmarked fund for China Agriculture Research System (CARS-25), the Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables, China, and the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-IVFCAAS).

About author: ZHANG Yan, E-mail: zhangyanvob@163.com; TIAN Tian, E-mail: tiantian122333@yeah.net; #Correspondence WANG Shao-li, Tel/Fax: +86-10-82109518, E-mail: wangshaoli@caas.cn * These authors contributed equally to this study.

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ZHANG Yan, TIAN Tian, ZHANG Kun, ZHANG You-jun, WU Qing-jun, XIE Wen, GUO Zhao-jiang, WANG Shao-li. 2023.

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