Scientia Agricultura Sinica ›› 2009, Vol. 42 ›› Issue (1): 55-63 .doi: 10.3864/j.issn.0578-1752.2009.01.007

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Sequence Analysis of a DFR Gene from Fagopyrum dibotrys (D.Don) Hara

  

  1. 重庆市农业学校

  • Received:2008-04-08 Revised:1900-01-01 Online:2009-01-10 Published:2009-01-10

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Abstract:

【Objective】 This work was aimed to investigate the sequence characteristics of a DFR gene from Fagopyrum dibotrys (D.Don) Hara. 【Method】According to the obtained homologous probe from other plant’s DFRs, DFR gene was isolated from the F. dibotrys using the RACE (rapid amplification of cDNA ends) methods to scanning cDNA library; and bio-informatical analysis, and Southern blot were also made. 【Result】One DFR cDNA clones (FdDFR1) was cloned (GenBank accession No. EF522145/ EF522146). The comparison between cDNA and genomic DNA sequences revealed that FdDFR1 is composed of two exons and one intron. Southern blot analysis indicated that DFR belongs to a small gene family, and only one FdDFR1 copy in F. dibotrys genomes. A NADP binding site (VTGASGFVGSWLVMRLLEHGY) and a substrate specificity motif (TVNVEEKQKPVYDETCW SDVDFCR RV) were observed in the deduced amino acid sequence of FdDFR1 contained N-terminal region. 【Conclusion】It is concluded that FdDFR1 has the typical characteristics with other homologous genes.

Key words: F. agopyrum dibotrys (D.Don) Hara, dihydroflavonol 4-reductase, clone, flavonoid

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