Abstract:

【Objective】 This experiment was designed to assess the effect of different levels of chromium picolinate (CrPic) on oxidation in new-born porcine hepatocyte. 【Method】 Three-day-old new born piglets were chosen as hepatocyte donors, hepatocytes were isolated enzymatic and mechanical methods. Porcine hepatocytes were cultured in medium in which added 0, 8, 200, 400 μmol?L-1 of CrPic for 48 hours. The levels of reactive oxygen species (ROS), the contents of malondialdehyde (MDA) in hepatocytes, the activities of lactate dehydrogenase (LDH) in medium and DNA strand breaks (by comet assay) were examined to investigate the effect of CrPic on porcine hepatocytes. 【Result】 Compared with control, the treatment groups have no significant differences in ROS level in hepatocytes, LDH activities in medium and DNA strand breaks (P＞0.05), however, 8 μmol?L-1 CrPic was significantly decreased MDA content in hepatocytes (P＜0.05). Compared with 8 μmol?L-1 CrPic group, the other parameters were markedly increased in 400 μmol?L-1 CrPic group (P＜0.05). Meanwhile, with the levels of CrPic increasing, analysis revealed a significant quadratic trend between CrPic and ROS (or LDH) (P＜0.05). 【Conclusion】 The appropriate amount of CrPic inhibits lipid peroxidation in cells, enhances antioxidation of porcine hepatocyte, however, 400 μmol?L-1 of CrPic has no effects on the antioxidation and oxidative damage.

Key words: chromium picolinate, pig, oxidation, porcine hepatocyte