Abstract:

【Objective】 A pair of primer was designed for detection of porcine circovirus type 2 from Shanghai, Jiangxi, Shandong, Jiangsu, Henan and Hunan in 2007. 【Result】 Results indicated that the positive percentage of PCV2 was 24.51% (63/257) and positive samples were detected from healthy pigs. Gene amplificated from PCR product was cloned and sequenced. Sequence analysis illustrated that nucleotide and amino acid homology of ORF2 of PCV2 isolates were 84.4%-100% and 82.5%-100%, respectively, and defferent belong to defferent groups . 【Conclusion】 There were two groups of PCV2 strains prevailing in China, the PCV2b group strains were predominant prevailing strain (70.97%, 22/31) , but the PCV2a group strains were prevailing also. There was no difference in genotype of PCV2 isolated from defferent geographical regions. Some PCV2 strains owned character of high pathogenic PCV2 implied that there was a high pathogenic PCV2 prevailing in China.

Key words: porcine circovirus type 2, ORF2, genotype