Scientia Agricultura Sinica ›› 2009, Vol. 42 ›› Issue (8): 2949-2957 .doi: 10.3864/j.issn.0578-1752.2009.08.038

• VETERINARY SCIENCE • Previous Articles     Next Articles

Molecular Epidemiology Analysis on Porcine Circovirus Type 2 in Some Regions of China in 2007

ZHANG Jian-wu, ZHUANG Jin-shan, LIU Chang-long, WANG Xiao-min, YUAN Shi-shan
  

  1. (中国农业科学院上海兽医研究所/中国动物卫生与流行病学中心上海分中心,动物传染病防治研究室/农业部动物寄生虫学重点开放实验室)
  • Received:2008-07-26 Revised:2009-02-12 Online:2009-08-10 Published:2009-08-10
  • Contact: YUAN Shi-shan

Abstract:

【Objective】 A pair of primer was designed for detection of porcine circovirus type 2 from Shanghai, Jiangxi, Shandong, Jiangsu, Henan and Hunan in 2007. 【Result】 Results indicated that the positive percentage of PCV2 was 24.51% (63/257) and positive samples were detected from healthy pigs. Gene amplificated from PCR product was cloned and sequenced. Sequence analysis illustrated that nucleotide and amino acid homology of ORF2 of PCV2 isolates were 84.4%-100% and 82.5%-100%, respectively, and defferent belong to defferent groups . 【Conclusion】 There were two groups of PCV2 strains prevailing in China, the PCV2b group strains were predominant prevailing strain (70.97%, 22/31) , but the PCV2a group strains were prevailing also. There was no difference in genotype of PCV2 isolated from defferent geographical regions. Some PCV2 strains owned character of high pathogenic PCV2 implied that there was a high pathogenic PCV2 prevailing in China.

Key words: porcine circovirus type 2, ORF2, genotype

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