苹果纯合基因型新种质的性状鉴定与培养

doi:10.3864/j.issn.0578-1752.2018.10.015

Abstract

Abstract:

【Objective】 Homozygous genotype lines were obtained by anther culture of different apple pollen grains, so their botanical characteristics, biological characteristics, and their induced culture, root conditions were different. In this study, the ploidy, characteristics and root conditions of each line were observed and analyzed. Appropriate rooting conditions and good combinations of lines for selecting apple homozygous genotypes are of great significance to the innovation of apple germplasm resources.【Method】 The homozygous genotype lines of ‘Gala’, ‘Fuji’ and ‘Red Star’ were obtained by apple anther culture. The ploidy level of homozygous genotype lines was studied using flow cytometry, root and leaf morphology and root conditions of each line were observed and recorded. 【Result】 A total of 32 homozygous lines were obtained by apple anther culture. The results of flow cytometry showed that there were 1 haploid, 1 triploids, 3 tetraploids and 27 diploids. The ploidy rate of 'Red Star' homozygous genotype was the highest, about 28.57%. The root rate, root length and root number of homozygous lines were affected by IBA concentration of rooting medium. When the concentration of IBA was 2-3 mg·L^-1, the root features of apple homozygous lines were the best. The leaf number, root length, root number, plant height, leaf length/width and petiole length of each line were different. ‘Red Star’ homozygous genotype lines (DH2-1, DH0-3, DH0-4, DH0-7), ‘Fuji’ homozygous genotype line (DH1-3) and ‘Gala’ homozygous genotype lines (DH2-2, DH2-4, DH2- 12, DH2-20) have a good traits (plants are taller, roots are longer, and the number of roots and leaves are more). The survival rate of ‘Red Star’ homozygous genotype was the highest, about 28.57%. 【Conclusion】 The main ploidy of apple homozygous genotype was diploid. Compared with the diploid lines, the haploid lines had a narrow leaf base, finer petiole, the leaf color and the leaf margin were shallow, while the polyploid lines showed wider leaf base, thicker petioles, leaf color and leaf edge serrated deep.

Key words: apple anther culture, ploidy analysis, homogeneous genotype, root hormone, trait analysis

JiangPeng SHI, ChunFen ZHANG, Shu DENG, LiYuan HOU, Rong XIAO, FuRong LI, YanHui DONG, YuanJun NIE, YiXue WANG, QiuFen CAO. Morphological Identification and Cultivation of New Germplasm of Apple Homozygous Genotypes[J].Scientia Agricultura Sinica, 2018, 51(10): 1960-1971.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2018.10.015

https://www.chinaagrisci.com/EN/Y2018/V51/I10/1960

Figures/Tables 12

Table 1

Fig. 1

Table 2

Fig. 2

Table 3

Table 4

Effects of different IBA concentrations on root morphology of ‘Gala’ apple homozygous lines"

根系形态 Root morphology	培养基 Medium	株系 Line
根系形态 Root morphology	培养基 Medium	DH2-1	DH2-2	DH2-3	DH2-4	DH2-5	DH2-6	DH2-7	DH2-8	DH2-9	DH2-10
生根率 Root rate (%)	T1	6.67	100	20	_	6.67	80	100	53.33	66.67	20
	T2	60	100	66.67	100	_	100	100	73.33	86.67	60
	T3	13.33	90	80	86.67	13.33	100	100	73.33	86.67	86.67
根长 Root length (cm.plant-1)	T1	4.65±0.97a	1.70±0.67b	2.93±0.49b	_	5.9a	4.27±0.88b	0.57±0.39b	32.60±1.05a	9.50±1.25a	7.53±1.31b
	T2	6.43±1.47a	1.63±0.78b	1.87±1.19b	29.43±1.80a	_	8.90±1.07a	2.50±1.02a	30.00±1.10a	8.93±1.25a	8.23±1.76b
	T3	1.5a	5.77±1.17a	13.17±1.20a	14.83±1.40b	5.85±1.18a	9.40±1.15a	3.67±0.71a	31.33±1.28a	10.93±1.54a	22.10±1.31a
根数 Root number	T1	1.50±0.89b	24.67±1.65b	3.67±1.15a	_	1a	9.33±0.83c	16c	9.00±1.20b	5.67±1.45a	1.33±0.83b
	T2	17.00±1.66a	39.67±1.67ab	1.67±0.83a	4.00±1.38a	_	42.33±1.82a	29.33±1.83b	21.67±1.61a	9.67±1.45a	5.00±1.20b
	T3	1b	51.67±2.42a	2.33±0.83a	6.00±1.38a	1a	34.67±1.28b	43.67±1.73a	8.00±1.59b	12.00±1.74a	22.33±1.86a
根系形态 Root morphology	培养基 Medium	株系 Line
根系形态 Root morphology	培养基 Medium	DH2-11	DH2-12	DH2-13	DH2-14	DH2-15	DH2-16	DH2-17	DH2-18	DH2-19	DH2-20
生根率 Root rate(%)	T1	66.67	20	80	6.67	53.33	_	6.67	93.33	73.33	6.67
	T2	100	100	100	73.33	66.67	20	53.33	100	73.33	86.67
	T3	93.33	53.33	100	60	53.33	_	13.33	100	93.33	66.67
根长 Root length (cm.plant-1)	T1	14.5±1.62b	12.7±1.26b	1.33±0.69c	4.8b	14.27±1.46a	_	4.5b	7.60±1.30c	13.43±1.54a	13.8a
	T2	20.7±1.40a	35.27±1.78a	3.83±1.18b	17.33±1.63a	10.23±1.25a	3.43±1.02	11.77±1.40a	16.10±1.72b	14.43±1.38a	18.40±1.57a
	T3	20.7±1.40a	23.2±1.90b	6.27±0.96a	6.40±1.36b	9.80±1.26a	_	3.20±1.46b	23.83±1.42a	11.07±1.33a	14.77±1.48a
根数 Root number	T1	3.00±1.20b	2.33±0.83b	11.67±0.83b	1a	13.67±1.36a	_	1b	5.00±1.00b	3.33±1.15b	27c
	T2	10.33±1.36a	11.33±1.15a	18.67±1.59ab	6.33±1.36a	7.00±1.63b	1.33±0.83	2.67±0.83a	8.33±1.67ab	3.67±1.15b	86.67±2.05a
	T3	5.33±1.59ab	3.00±1.20b	22.33±1.71a	5.33±1.48a	5.00±1.26b	_	1.50±0.89ab	14.33±1.61a	16.00±1.53a	59.67±2.50b

Table 4

Fig. 3

Table 5

Table 6

Table 7

Plant traits of ‘Gala’ apple homozygous genotype"

株系 Line	叶数 Leave number	根数 Root number	根长 Root length(cm)	株高 Plant height	叶柄长 Petiole length (cm)	叶形指数 Leaf shape index
DH2-1	13.67±1.48gh	17.00±1.66ef	6.43±1.47ghi	1.70±0.46g	0.70±0.46ef	2.15±0.60bc
DH2-2	20.00±1.00defg	41.67±1.52b	6.33±1.01ghi	5.57±1.01a	1.27±0.59ab	2.29±0.57b
DH2-3	32.33±1.83abc	3.67±1.15hij	13.17±1.20f	3.17±0.71d	1.0±0.67bcde	3.07±0.68a
DH2-4	36.67±2.30a	5.00±1.00ghij	27.17±2.08bc	2.97±0.86de	1.33±0.39a	1.74±0.34defg
DH2-5	11.33±1.36h	1j	5.87±1.05ghi	1.70±0.70g	0.57±0.39f	1.69±0.75efg
DH2-6	21.33±1.28defg	35.67±1.15c	9.97±0.98fgh	3.20±0.71d	0.80±0.64cdef	2.21±0.45b
DH2-7	18.67±1.28defgh	43.67±1.72b	35.27±1.78a	5.10±0.58ab	1.10±0.46abc	2.07±0.51bcd
DH2-8	20.67±1.93defg	21.67±1.61de	3.67±0.71i	1.67±0.53g	0.80±0.64cdef	1.92±0.68bcdef
DH2-9	19.33±1.45defgh	8.00±1.26ghi	11.67±1.40fg	4.10±0.46c	0.83±0.53cdef	1.63±0.56fg
DH2-10	20.00±1.53defg	5.00±1.20ghij	22.10±1.31cd	2.20±0.64fg	0.83±0.49cdef	1.92±0.54bcdef
DH2-11	17.33±1.05efgh	10.00±1.44gh	12.53±1.31f	2.90±0.64de	0.90±0.46cde	1.40±0.45g
DH2-12	27.00±1.53bcd	11.33±1.15fg	19.63±1.36de	2.90±0.64de	0.73±0.53def	1.58±0.47fg
DH2-13	21.67±1.05defg	24.67±1.28d	4.93±1.47hi	1.77±0.53g	0.70±0.46ef	2.21±0.37b
DH2-14	25.00±1.38cde	6.33±1.36ghij	9.17±1.46fghi	2.53±0.53def	0.83±0.63cdef	2.12±0.59bc
DH2-15	20.67±1.36defg	4.00±1.00hij	14.89±1.28ef	2.47±0.39ef	0.93±0.39cde	1.80±0.55cdef
DH2-16	15.33±1.36fgh	1.33±0.83j	3.43±1.02i	3.17±0.59d	1.03±0.39bcd	2.29±0.59b
DH2-17	21.00±1.26defg	2.67±0.83ij	11.77±1.40fg	2.40±0.56ef	0.83±0.49cdef	2.11±0.39bc
DH2-18	34.33±1.78ab	11.00±1.20fg	9.87±1.03fgh	3.07±0.67de	0.80±0.58cdef	2.07±0.49bcd
DH2-19	25.00±1.38cde	11.33±1.68fg	11.17±1.31fg	3.17±0.71d	0.90±0.46cde	2.00±0.44bcde
DH2-20	23.67±1.61def	85.67±2.16a	30.00±1.10b	4.60±0.67bc	1.03±0.53bcd	2.21±0.53b

Table 7

Fig. 4

Fig. 5

References 38

[1]	GERMANÀ M A.oubled haploid production in fruit crops. DPlant Cell, Tissue and Organ Culture, 2016, 86(2): 131-146.
[2]	王炜, 陈琛, 欧巧明, 叶春雷, 罗俊杰. 小麦花药培养的研究和应用. 核农学报, 2016, 30(12): 2343-2354. doi: 10.11869/j.issn.100-8551.2016.12.2343
	WANG W, CHEN C, OU Q M, YE C L, LUO J J.Research and application of wheat anther culture.Journal of Nuclear Agricultural Sciences, 2016, 30(12): 2343-2354. (in Chinese) doi: 10.11869/j.issn.100-8551.2016.12.2343
[3]	CARDOSO J C, MARTINELLI A P, GERMANÀ M A, LATADO R R.In vitro anther culture of sweet orange (Citrus sinensis L. Osbeck) genotypes and of a C. clementina × C. sinensis ‘Hamlin’ hybrid. Plant Cell, Tissue and Organ Culture, 2014, 117(3): 455-464.
[4]	ZHAO J, ZOU X X, ZHANG Z Q, YANG B Z, ZHOU S D.Influences of carbon sources and plant growth regulators on anther culture efficiency of pepper.Agricultural Science and Technology, 2010, 11(4): 102-105.
[5]	HÖFER M.In vitro androgenesis in apple. Gartenbauwissenschaft, 2003, 60(1): 287-292.
[6]	李佳. 百合单倍体培养及细胞学观察[D]. 北京: 中国农业科学院, 2011.
	LI J.Haploid culture and cytology observation in lilium [D]. Beijing: Chinese Academy of Agricultural Sciences, 2011. (in Chinese)
[7]	ZHANG C F, SATO S, TSUKUNI T, SATO M, OKADA H, YAMAMOTO T, WADA M, MATSUMOTO S, YOSHIKAWA N, MIMIDA N, TAKAGISHI K, WATANABE M, CAO Q F, KOMORI S.Elucidating cultivar differences in plant regeneration ability in an apple anther culture.The Horticulture Journal preview, 2016, doi: 10.2503/ hortj.MI-094.
[8]	薛光荣, 牛健哲, 杨振英, 史永忠, 费开韦. 苹果花药培养技术及8个主栽品种的花粉植株培育成功. 中国农业科学, 1990, 23(3): 86-87.
	XUE G R, NIU J Z, YANG Z Y, SHI Y Z, FEI K W.The technique of apple anther culture and the successful culture of pollen plantlets of 8 main apple cultivar.Scientia Agricultura Sinica, 1990, 23(3): 86-87. (in Chinese)
[9]	ZHANG C, TSUKUNI T, IKEDA M, SATO M, OKADA H, OHASHI Y, MATSUNO H, YAMAMOTOT, WADA M, YOSHIKAWA N, MATSUMOTO S, LI J, MIMIDA N, WATANABE M, SUZUKI A, KOMORI S.Effects of the microspore development stage and cold pre-treatment of flower buds on embryo induction in apple (Malus × domestica Borkh.) anther culture. Journal-Japanese Society for Horticultural Science, 2013, 82(82): 114-124.
[10]	温鑫, 邓舒, 张春芬, 侯丽媛, 石江鹏, 聂园军, 肖蓉, 秦永军, 曹秋芬. ‘嘎啦’苹果花药培养种质创新. 中国农业科学, 2017, 50(14): 2793-2806. doi: 10.3864/j.issn.0578-1752.2017.14.015
	WEN X, DENG S, ZHANG C F, HOU L Y, SHI J P, NIE Y J, XIAO R, QIN Y J, CAO Q F.Regeneration of new germplasms using anther culture of apple cultivar ‘Gala’.Scientia Agricultura Sinica, 2017, 50(14): 2793-2806. (in Chinese) doi: 10.3864/j.issn.0578-1752.2017.14.015
[11]	HÖFER M. Regeneration of androgenic embryos in apple (Malus× domestica Brokh.) via anther and microspore culture. Acta Physiologiae Plantarum, 2005, 27(4): 709-716. doi: 10.1007/s11738-005-0075-6
[12]	HÖFER M, GRAFE C, BOUDICHEVSKAJA A, LOPEZ A, BUENO M A, ROEN D. Characterization of plant material obtained by in vitro androgenesis and in situ parthenogenesis in apple. Scientia Horticulturae, 2008, 117(3): 203-211. doi: 10.1016/j.scienta.2008.02.020
[13]	HÖFER M, FLACHOWSKY H. Comprehensive characterization of plant material obtained by in vitro androgenesis in apple.Plant Cell, Tissue and Organ Culture, 2015, 122(3): 617-628. doi: 10.1007/s11240-015-0794-3
[14]	翟衡, 史大川, 束环瑞. 我国苹果产业发展现状与趋势. 果树学报, 2007, 24(3): 355-360.
	ZHAI H, SHI D C, SHU H R.Current state and developing trend of apple industry in China.Journal of Fruit Science, 2007, 24(3): 355-360. (in Chinese)
[15]	张春芬, 邓舒, 肖蓉, 孟玉平, 曹秋芬. 苹果花药培养再生植株的倍性鉴定及SSR分析. 园艺学报, 2015, 42(S1): 2580.
	ZHANG C F, DENG S, XIAO R, MENG Y P, CAO Q F.Ploidy identification and SSR analysis of regenerated plants in apple anther culture.Acta Horticulturae Sinica, 2015, 42(S1): 2580. (in Chinese)
[16]	任莹. 苹果单倍体育种技术探究[D]. 太原: 山西大学, 2015.
	REN Y.Research on Haploid breeding technique in anther culture [D]. Taiyuan: Shanxi University, 2015. (in Chinese)
[17]	蒲富慎.果树种质资源描述符—记载项目及评价标准. 北京: 中国农业出版社, 1990: 23-37.
	PU F S.Fruits Germplasm Descripto—Record Items and Evaluation Standards. Beijing: China Agricultural Press, 1990: 23-37. (in Chinese)
[18]	崔佩佩, 刘鹏, 刘佳琪, 王劲松, 武爱莲, 董二伟, 丁玉川, 焦晓燕. 不同养分配比对高粱根系生长及养分吸收的影响. 中国生态农业学报, 2017, 25(11): 1643-1652. doi: 10.13930/j.cnki.cjea.170300
	CUI P P, LIU P, LIU J Q, WANG J S, WU A L, DONG E W, DING Y C, JIAO X Y.Effect of different nutrient co mbinations on root growth and nutrient accumulation in sorghum.Chinese Journal of Eco- Agriculture, 2017, 25(11): 1643-1652. doi: 10.13930/j.cnki.cjea.170300
[19]	ZENG S H, CHEN C W, HONG L, LIU J H, DENG X X.n vitro induction, regeneration and analysis of autotetraploids derived from protoplasts and callus treated with colchicine in Citrus. Plant Cell, Tissue and Organ Culture, 2006, 87(1): 85-93. doi: 10.1007/s11240-006-9142-y
[20]	KAWASE K, YAHATA M, NAKAGANA S, HARAGUCHI K, KUNITAKE H.Selection of autotetraploid and its morphological characteristics in Meiwa Kumquat (Fortunella crassifolia Swingle)(Breeding & Germplasm Resources). Horticultural Research, 1975, 4(11): 141-146.
[21]	HÖFER M.In vitro androgenesis in apple-improvement of the induction phase. Plant Cell Reports, 2004, 22(6): 365-370. doi: 10.1007/s00299-003-0701-y pmid: 14685764
[22]	TESTILLANO P, GEORGIEV S, MOGENSEN H L, CORONADO M J, DUMAS C, RISUENO M C, MATTHYS-ROCHON E.Spontaneous chromosome doubling results from nuclear fusion during in vitro maize induced microspore embryogenesis.Chromosoma, 2004, 112(7): 342-349. doi: 10.1007/s00412-004-0279-3 pmid: 15138769
[23]	GRIGGS R, ZHENG M Y.Nuclear fusion during early stage of microspore embryogenesis indicates chromosome doubling in wheat (Triticum aestivum). American Journal of Plant Sciences, 2016, 07(3): 489-499.
[24]	钟晓红, 戴思慧, 马定渭. 核果类果树茎尖培养研究进展. 果树学报, 2003, 20(5): 388-392. doi: 10.3969/j.issn.1009-9980.2003.05.011
	ZHONG X H, DAI S H, MA D W.Advances of research in shoot-tip culture on stone fruit crops.Journal of Fruit Science, 2003, 20(5): 388-392. (in Chinese) doi: 10.3969/j.issn.1009-9980.2003.05.011
[25]	肖祖飞. 童性对苹果砧木绿枝扦插生根的影响[D]. 北京: 中国农业大学, 2014.
	XIAO Z F.Impact of the juvenility on the adventitious rooting of leafy cuttings in apple rootstocks [D]. Beijing: China Agriculture University, 2014. (in Chinese)
[26]	KAUSHAL N, MODGIL M, THAKUR M, SHARMA D R .In vitro clonal multiplication of an apple rootstock by culture of shoot apices and axillary buds. Indian Journal of Experimental Biology, 2005, 43(6): 561-565. pmid: 15991584
[27]	DOBRÁNSZKI J, SILVA J A T D. Micropropagation of apple-a review.Biotechnology Advances, 2010, 28(4): 462-488. doi: 10.1016/j.biotechadv.2010.02.008
[28]	谢璇, 许轲, 谢闽新, 朱元娣. 苹果茎尖培养快繁体系的优化. 植物生理学报, 2015, 51(12): 2152-2156. doi: 10.13592/j.cnki.ppj.2015.0474
	XIE X, XU K, XIE M X, ZHU Y D.Optimization of rapid micropropagation system of apple meristem-tip culture.Plant Physiology Journal, 2015, 51(12): 2152-2156. (in Chinese) doi: 10.13592/j.cnki.ppj.2015.0474
[29]	陈银全, 沈汉清, 柯昉. 水稻花培育种研究及新进展. 福建农业学报, 1997, 12(2): 6-10.
	CHEN Y Q, SHEN H Q, KE F.Studies and advances on rice anther culture breeding.Journal of Fujian Academy of Agricultural Sciences, 1997, 12(2): 6-10. (in Chinese)
[30]	VANWYNSBERGHE L, KDE W, COART E, KEULEMANS J.Limited application of homozygous genotypes in apple breeding.Plant Breeding, 2005, 124(4): 399-403. doi: 10.1111/j.1439-0523.2005.01117.x
[31]	OKADA H, OHASHI Y, SATO M, MATSUNO H, YAMAMOTO T, KIM H, TUKUNI T, KOMORI S.Characterization of fertile homozygous genotypes from anther culture in apple.Journal of the American Society for Horticultural Science, 2009, 134(6): 641-648. doi: 10.1051/fruits:2009034
[32]	CAO H, BISWAS MK, LU¨ Y, AMAR MH, TONG Z, XU Q, XU J, GUO W, DENG X.Doubled haploid callus lines of valencia sweet orange recovered from anther culture.Plant Cell, Tissue and Organ Culture, 2011, 104(3): 415-423. doi: 10.1007/s11240-010-9860-z
[33]	GERMANA M A, ALEZA P, CARRERA E, CHEN C, CHIANCONE B, COSTANTINO G, DAMBIER D, DENG X X, FEDERICI C T, FROELICHER Y, GUO W W, IBÁÑEZ V, JUÁREZ S, KWOK K, LURO F, MACHADO M A, NARANJO M A, NAVARRO L, OLLITRAULT P, RÍOS G, ROOSE M L, TALON M, XU Q, GMITTER JR F G. Cytological and molecular characterization of three gametoclones of Citrus clementina.BMC Plant Biology, 2013, 13(1): 129-150. doi: 10.1186/1471-2229-13-129 pmid: 3847870
[34]	LI Y, LI H, CHEN Z, JI L X, YE M X, WANG J, WANG L, AN X M.Haploid plants from anther cultures of poplar (Populus × beijingensis). Plant Cell, Tissue and Organ Culture, 2013, 114(1): 39-48.
[35]	CHEN Z J,HA M,SOLTIS D.Polyploidy: Genome obesity and its consequences.New Phytologist, 2007, 174(4): 717-720. doi: 10.1111/j.1469-8137.2007.02084.x pmid: 17504455
[36]	ADAMS K L, WENDEL J F.Novel patterns of gene expression in polyploidy plants.Trends in Genetics, 2005, 21(10): 539-543.
[37]	HA M, KIM E D, CHEN Z J.Duplicate genes increase expression diversity in closely related species and allopolyploids.Proceedings of the National Academy of Sciences, 2009, 106(7): 2295-2300.
[38]	RIDDLE N C, KATO A, BIRCHLER J A.Genetic variation for the response to ploidy change in Zea mays L. Theoretical and Applied Genetics, 2006, 114(1): 101-111. doi: 10.1007/s00122-006-0414-z pmid: 17053922

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

红星株系 Red star strain	倍性 Ploidy	富士株系 Fuji strain	倍性 Ploidy	嘎啦株系 Gala strains	倍性 Ploidy	嘎啦株系 Gala strains	倍性 Ploidy	嘎啦株系 Gala strains	倍性 Ploidy
DH0-1	2n	DH1-1	2n	DH2-1	2n	DH2-8	2n	DH2-15	4n
DH0-2	2n	DH1-2	4n	DH2-2	2n	DH2-9	2n	DH2-16	2n
DH0-3	2n	DH1-3	2n	DH2-3	1n	DH2-10	2n	DH2-17	2n
DH0-4	2n	DH1-4	2n	DH2-4	2n	DH2-11	2n	DH2-18	2n
DH0-5	4n	DH1-5	2n	DH2-5	2n	DH2-12	2n	DH2-19	2n
DH0-6	2n			DH2-6	2n	DH2-13	2n	DH2-20	2n
DH0-7	3n			DH2-7	2n	DH2-14	2n

根系形态 Root morphology	培养基 Medium	株系 Line
根系形态 Root morphology	培养基 Medium	DH0-1	DH0-2	DH0-3	DH0-4	DH0-5	DH0-6	DH0-7
生根率 Root rate (%)	T1	26.67	6.67	70	46.67	33.33	46.67	33.33
	T2	53.33	86.67	100	93.33	86.67	66.67	100
	T3	73.33	73.33	93.33	93.33	100	53.33	66.67
根长 Root length (cm/plant)	T1	5.70±0.81a	4.80b	18.13±1.53a	3.70±1.16a	3.50±0.58b	3.50±0.79a	3.90±0.56a
	T2	3.87±1.13a	6.63±1.19a	20.83±1.66a	3.73±0.77a	4.63±0.91b	3.17±0.77a	3.93±0.67a
	T3	4.60±0.71a	4.50±0.67b	20.13±1.51a	4.07±1.13a	8.33±1.17a	3.13±0.90a	4.27±0.95a
根数 Root number	T1	7.00±1.26b	3.00b	5.00±1.00b	2.33±1.05c	3.33±0.83b	3.33±1.32b	1.00c
	T2	4.67±1.32b	5.00±1.00a	10.00±1.26a	15.33±1.28a	5.67±1.05a	9.00±1.00a	22.33±1.59a
	T3	17.67±1.56a	1.33±0.83c	7.00±1.26ab	9.33±0.83b	6.33±1.15a	8.67±0.83a	12.33±1.45b

株系 Line	叶数 Leave number	根数 Root number	根长 Root length (cm)	株高 Plant height (cm)	叶柄长 Petiole length (cm)	叶形指数 Leaf shape index
DH0-1	27.00±1.00a	17.67±1.56a	4.27±0.91a	3.70±0.91ab	0.83±0.49a	1.66±0.52b
DH0-2	12.33±1.45b	4.33±1.28c	5.73±1.15b	2.37±0.80c	0.5±0.46c	2.07±0.49a
DH0-3	22.33±1.28a	10.67±1.28b	18.17±1.89a	4.13±0.80a	0.80±0.46a	1.60±0.51b
DH0-4	26.67±1.71a	18.67±1.56a	3.17±0.85b	3.47±0.71abc	0.87±0.39a	1.74±0.54b
DH0-5	15.00±1.00b	8.00±1.00bc	3.53±0.91b	2.57±0.86bc	0.63±0.39bc	1.71±0.37b
DH0-6	26.00±1.84a	11.00±1.38b	2.87±0.49b	4.57±0.49a	0.7±0.46b	1.68±0.59b
DH0-7	22.67±1.28a	22.33±1.59a	2.67±1.08b	3.67±1.01ab	0.77±0.39a	1.88±0.67ab

株系 Line	叶数 Leave number	根数 Root number	根长 Root length(cm)	株高 Plant height (cm)	叶柄长 Petiole length (cm)	叶形指数 Leaf shape index
DH1-1	15.33±1.32b	17.67±1.56b	7.93±0.87b	1.97±0.67d	0.83±0.39bc	1.90±0.63ab
DH1-2	16.00±1.38b	12.33±1.28c	22.27±1.63a	2.23±0.63cd	1.53±0.77a	1.61±0.41b
DH1-3	17.67±1.15ab	26.00±1.53a	19.00±1.56a	3.07±0.69ab	1.17±0.53ba	1.58±0.73b
DH1-4	22.67±1.28a	4.00±1.38d	4.47±1.32b	2.57±0.59bc	0.87±0.39bc	2.12±0.55a
DH1-5	22.33±1.65a	1.33±0.83d	4.43±1.55b	3.33±0.72a	0.70±0.58c	1.52±0.57b

Morphological Identification and Cultivation of New Germplasm of Apple Homozygous Genotypes

RichHTML

PDF

Abstract

Cite this article

share this article

Figures/Tables 12

References 38

Related Articles 2

Metrics

Comments

Recommended 0

[1]	WEN Xin, DENG Shu, ZHANG ChunFen, HOU LiYuan, SHI JiangPeng, NIE YuanJun, XIAO Rong, QIN YongJun, CAO QiuFen. Regeneration of New Germplasms Using Anther Culture of Apple Cultivar ‘Gala’ [J]. Scientia Agricultura Sinica, 2017, 50(14): 2793-2806.
[2]	JIE Kai-Dong-1, WANG Hui-Qin-1, WANG Xiao-Pei-1, LIANG Wu-Jun-1, XIE Zong-Zhou-1, YI Hua-Lin-1, DENG Xiu-Xin-1, Grosser Jude W2, GUO Wen-Wu-1. Extensive Citrus Triploid Breeding by Crossing Monoembryonic Diploid Females with Allotetraploid Male Parents [J]. Scientia Agricultura Sinica, 2013, 46(21): 4550-4557.