Scientia Agricultura Sinica ›› 2005, Vol. 38 ›› Issue (01): 213-216 .doi: 10.3864/j.issn.0578-1752.as-2003-1769

• RESEARCH NOTES • Previous Articles    

Studies on the Methods of Detecting PVYN and PVYO by RT-PCR

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  1. 山东农业大学农学院
  • Received:2003-09-17 Revised:1900-01-01 Online:2005-01-10 Published:2005-01-10

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Abstract: Potato virus Y(PVY) is one of the important viruses infecting potatoes. It influences the production seriously. The nucleotide sequences of potato virus Y N-strain（PVYN）and O-strain（PVYO） were compared. A pair of homologous primers were designed according to the conserved region of coat protein gene. So the RT-PCR method of detecting PVY was built. Judging by the result of comparison, the 3′primer was designed in conserved region and two 5′primers were designed in two variable region. So a complex RT-PCR method was built which could distinguish PVYN and PVYO. The two methods were very powerful for detecting virus-free seed potatoes, investigating PVY and studying its pathology.

Key words: Potato virus Y(PVY), RT-PCR, Detecting

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