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Table of Content

    , Volume 38 Issue 01
      
    CROP GENETICS & BREEDING·GERMPLASM RESOURCES
    Analysis on Stability of AC, GT and PC in Rice Varieties(Orzya sativa L.)
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    Scientia Agricultura Sinica. 2005, 38(01):  1-6 . 
    Abstract ( 1169 )   PDF (430KB) ( 1320 )   Save
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    The stability of amylose content (AC), gelatinization temperature (GT) and protein content (PC) of rice varieties were analyzed using additive main effects and multiplicative interaction (AMMI) model in two years and three sites. Eighteen tested varieties were further clustered and evaluated based on the phenotypic values of three quality traits and their Di value from the AMMI analysis. The results showed that the genotype by environmental interactions, the difference in phenotype and stability of AC, GT and PC among different genotypes and environments were all significant at 1% level. Also, only one variety, W002, had high stability for all the three quality traits. Additionally, in consideration of AC, GT and PC, and their stability in rice varieties, etc, four rice varieties, W002, Zaofeng 9, Guanglingxiangjing and Nanjing16, could be also adopted as breeding parents to improve eating quality and stability of elite rice.
    Study on the Genetic Diversity of Wheat by EST-SSRs
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    Scientia Agricultura Sinica. 2005, 38(01):  7-12 . 
    Abstract ( 1198 )   PDF (402KB) ( 972 )   Save
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    EST-SSR marker is a new kind of SSR markers which is developed from the ESTs (expressed sequence tags). In this study, a total of 35 EST-SSR markers were used to analyze the genetic diversity and phylogeny of 96 wheat germplasms. All the primer sets produced clear and strong expected amplifications. 129 alleles were generated by the 35 loci, but most of the primer pairs produced 2 to 4 alleles. 87.6% of the 129 alleles showed polymorphic among the 96 wheat varieties. Primer pairs could be used to identify some varieties according to the specific PCR amplifications, to reveal the phylogeny between hexaploid wheat collections and their ancestral chromosome donors. Based on 0.745 similarity coefficient, synthesized double diploid varieties and hexaploid wheat were classified into different subgroups. Compared with SSR markers derived from genomic DNAs, EST-SSRs were parts of expressed genes. Therefore, when used in wheat genetic analysis, EST-SSR markers not only act as genetic markers, but also reveal differences in related gene expression.
    Effect of Genotype and Location on Polyphenol Oxidase Activity in Wheat Flour
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    Scientia Agricultura Sinica. 2005, 38(01):  13-16 . 
    Abstract ( 882 )   PDF (220KB) ( 762 )   Save
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    Polyphenol oxidase (PPO) is related to undesirable brown discoloration of wheat-based end products such as noodle and steamed bread. In total, 22 main cultivars sown in 11 locations in middle and lower Yangtze Valleys in 1999-00 season were used to investigate the effects of genotype, environment and other quality characteristics on flour PPO activity. The variability in flour PPO activities was influenced by growing location, genotype and their interaction significantly. Flour PPO activities were related to flour L*, with correlation coefficient -0.55. Grain hardness, flour b*, Zeleny sedimentation value, Farinograph water absorption and dough stability were significantly correlated with flour PPO activity, with r = 0.57, 0.69, 0.46, 0.42 and 0.46, respectively. Significant negative correlations were found between PPO activity and test weight and starch pasting character.
    Innovation of Super-Prematurity New Millet Idioplasm with Sweet Straw and Multi-Spike
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    Scientia Agricultura Sinica. 2005, 38(01):  17-21 . 
    Abstract ( 854 )   PDF (228KB) ( 856 )   Save
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    A new millet (Setaria italica Beauv) varity, super-early maturity No.1, with sweet-stalk and multi-spike was developed through several years of study by using target character gene bank in breeding. This variety has five outstanding characters. Firstly, super-early-maturity, the variety only need 1550℃ effective accumulated temperature and can normally mature in the Bashang Region in Hebei Province, thus could break through the forbidden zone of millet cultivation and move the margin of millet growth northward greatly. Secondly, this variety is a rare idioplasm of multi-spike, its branch can grow into spike and the burl in its middle and lower part can outgrow several spikes. Thirdly, its straw is sweet and has a high content of sugar. The whole-sugar, dissolvable sugar and deoxidized sugar in its straw are 74.8%, 200.5% and 237.2% higher than the common varieties, respectively, in the same region. Fourthly, high-content crude protein, which is higher than the common varieties by 3.9% to 30.4%. Fifthly, the color of grain is changeable. The grain of super-early-maturity No.1 is red in Shijiazhuang, but yellow in Bashang region. Also, this variety possesses the character of high quality and yield. In this article, the innovation way is introduced.
    Discovery of a Dominant Nuclear Male Sterile Mutant N7241S in Soybean and Analysis of Its Inheritance
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    Scientia Agricultura Sinica. 2005, 38(01):  22-26 . 
    Abstract ( 801 )   PDF (316KB) ( 694 )   Save
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    A spontaneous soybean male-sterile mutant N7241S was discovered and identified from a landrace Fuyangsilijia (N7241). The results of pollen germination experiments showed that the pollens of N7241S plants were completely aborted, while the female fertility of the N7241S sterile plants was normal according to parallel cross tests. The male-sterile plants performed relatively better in outcrossing ability than those of recessive monogenic mutants, such as ms1, ms2, etc. Therefore, N7241S is a male-sterile female-fertile mutant. Genetical analyses of different segregational populations between N7241S male-sterile plants and five normal parents showed that the male sterility of N7241S was controlled by a dominant gene. Strategies and methods of N7241S dominant male sterility to be used in recurrent selection as well as hybrid seed production were discussed.
    Morphological Diversity and Classification of Common Bean (Phaseolus vulgaris L.) Germplasm Resource in China
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    Scientia Agricultura Sinica. 2005, 38(01):  27-32 . 
    Abstract ( 1161 )   PDF (320KB) ( 805 )   Save
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    The morphological diversity and classification of 324 common bean (Phaseolus vulgaris L.) germplasm resources were researched in the present study. The results showed that the morphological diversity was high among the accessions assessed. The average diversity index (1.632) was 40.5% higher than that of alien common bean germplasm. The first and second principal components totally represented 44% of morphological diversity. Based on the morphological data, 280 Chinese accessions were clustered into 3 groups. The accessions of the first group had shorter growth period and plant height, bigger seed size, less pods per plant and seeds per pod and determinate growth habit, etc. They were mainly from northeastern and northern regions of China. The accessions in the third group had longer growth period and plant height, smaller seed size, more pods per plant and seeds per pod and indeterminate growth habit, etc. They were mainly from southwestern. The main characters of second group were between the first and the third group. They was distributed widely.
    1960-2004
    Genetic Origins of Differentially Expressed Genes Under Photoinhibition Stress in Super Hybrid Rice Liangyoupeijiu
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    Scientia Agricultura Sinica. 2005, 38(01):  33-37 . 
    Abstract ( 2318 )   PDF (324KB) ( 156 )   Save
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    The mRNA differential display visualized by silver staining was applied to study genes expressed differentially under stress condition of photoinhibition in super hybrid rice Liangyoupeijiu and its female parent Peiai 64S and male parent 9311. One hundred and sixty-seven differentially displayed cDNA fragments of hybrid rice were obtained and their genetic origins were studied. The results suggested that most of the differentially expressed genes were inherited from both male and female parents rather than either of them because they existed in both male and female parents. Some of the differentially expressed gene fragments seemed to originate from a single-parent or from none of the parents, suggesting the existence of some unclear mechanisms.
    TILLAGE & CULTIVATION·PHYSIOLOGY & ECOLOGY
    A Knowledge Model for Design of Suitable Dynamics of Growth Index in Rice
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    Scientia Agricultura Sinica. 2005, 38(01):  38-44 . 
    Abstract ( 1057 )   PDF (510KB) ( 839 )   Save
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    Based on the analysis of the quantitative relationships of rice growth dynamics to varietal traits, eco-environments and planting measures, a universal knowledge model for rice was developed, which was driven by physiological development time-based cumulative temperature. The model can be used to predict the time-course dynamics of growth indices including population stem and tiller number, leaf area index and dry matter accumulation for growth guideline and diagnosis with different varieties, eco-environments and planting measures. Case studies with the data sets of normal climatic year and different variety types at different eco-sites indicated a good performance of the model system for prediction with the RMSE=5.0 for the simulated and observed population density, r=0.85** for the simulated and observed growth indices.
    Comparison of Enzymes Activity Associated with Sucrose Metabolism in the Developing Grains Between Sweet Corn and Normal Corn
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    Scientia Agricultura Sinica. 2005, 38(01):  52-58 . 
    Abstract ( 1089 )   PDF (428KB) ( 781 )   Save
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    Field experiments were carried out to compare the dynamic variations of enzymes activities of sucrose and starch metabolism in the developing grains of sweet corn (Zea mays L. Seccharata Sturt) and normal corn (Zea mays L. Indentata Sturt). The activities of SS (syntheses and degradation direction) and invertase in grains of sweet corn were higher than those of normal corn, so the increase of sucrose in sweet corn is resulted from the increase of sucrose synthetic ability, but not the reduction of sucrose degradation. The activities of ADPG-PPase, UDPG-PPase and SSS were higher in sweet corn than normal corn, but that of the SSS, SBE, SDE were lower than normal corn. The reductions of SDE activity lead to the accumulation of WSP. The decreases of total starch and amylose percent in total starch were resulted from the reduction of GBSS, SBE and SDE activities.
    Correlation Between Ultra-microstructure and Nutrition Quality in Opaque-2 Corn Kernels with Different Hardness Endosperm
    Scientia Agricultura Sinica. 2005, 38(01):  59-63 . 
    Abstract ( 974 )   PDF (265KB) ( 803 )   Save
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    Two quality protein maize populations (C. Pop 14QPM and Shaanzong 5QPM) and five classes (1, 2, 3, 4, 5) of endosperm hardness were used to study ultra-microstructure and chemical analysis of their kernels. Results indicated that the harder the endsoperm was, the better the physical characters were and the poorer the protein quality of kernds was. Under the opague-2 background, the differences of kernel density and endosperm hardness mainly experessed in internal endosperm tissue. Kernel physical characters were influenced by cell distribution, shape, homogeneity and texture of starch granules in internal endoperm tissue. Nutrition quality was influenced by the distribution, density and morphology of matrix protein, and the compact degree between starch granules and matrix protein.
    PLANT PROTECTION
    Molecular Mechanism of Amicarthiazol Resistance in Xanthomonas oryzae pv.oryzae
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    Scientia Agricultura Sinica. 2005, 38(01):  64-69 . 
    Abstract ( 1314 )   PDF (388KB) ( 895 )   Save
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    The resistance mutants of wild-type Xanthomonas oryzae pv.oryzae to amicarthiazol were got by ultraviolet(UV) irradiation in laboratory. These mutants could grow in the medium containing 100 μg·ml-1 of amicarthiaol. However the wild-type sensitive strain could not grow at 10 μg·ml-1. The activity of succinate dehydrogenase(SDH) was strongly inhibited by amicarthiazol while the activity of SDH from resistant mutants was very low and was not inhibited by the chemical. Six pairs of special primer based on the conserved region of Xanthomonas axonopodis pv.citri were designed and used to amplify the gene encoding succinate dehydrogenase from X.oryzae. Whole gene of succinate dehydrogenase has 3 616bp, 1 115 deduced amino acids and two introns. The homology of the nucleotide sequence and deduced amino acid of X. oryzae with that of X.citri was 93% and 97% respectively. Compared with that of other six bacteria, the homology of deduced amino acid of succinate dehydrogenase was from 43% to 95% respectively. Sequence comparison among sensitive and five mutants revealed that mutant gene encodes a modified iron-sulpher subunit of succinate dehydrogenase with a amino-acid substitution (His 229→Tyr).
    Analysis on Genetic Relationship of Oxya chinensis and O. japonica from Xuzhou and Pingshan
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    Scientia Agricultura Sinica. 2005, 38(01):  70-75 . 
    Abstract ( 1077 )   PDF (378KB) ( 644 )   Save
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    Genetic Relationships among Oxya chinensis and O. japonica populations collected from Xuzhou City, Jiangsu Province and Pingshan County , Hebei Province were analyzed by using random amplified polymorphic DNA (RAPD) markers. 125 clear and stable bands of 43 grasshopper individuals with molecular weight ranging from 200 to 2 200 bps revealed on the electropherogram amplified by 10 random primers,and 123 (99%) of this bands were polymorphic, Shannon 's index showed that the genetic diversity within O. chinensis(0.3432) was more higher than that of O. japonica (0.2781 ). Nei's genetic distance of O. chinensis and O. japonica from same area was less than that from two far apart areas. The dendrogram based on Nei's genetic distance of RAPD markers was constructed with UPGMA and Neighbor-Joining methods. Cluster analysis indicated that all the individuals were grouped into two main clusters,namely, O. chinensis from Xuzhou and Pingshan has been clustered into one cluster,while O. japonica from the two regions belonged to the other cluster. The results showed that RAPD can provide polymorphisms even at slight difference leval among individuals, indicated that RAPD is an effective molecular marker to distinguish the closed related species.
    Sequence Analysis of mtDNA COI Gene and Molecular Phylogeny of Different Geographical Populations of Bemisia tabaci (Gennadius)
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    Scientia Agricultura Sinica. 2005, 38(01):  76-85 . 
    Abstract ( 1380 )   PDF (338KB) ( 1181 )   Save
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    Bemisia tabaci(Gennadius) is a worldwide serious insect pest possessing a number of biotypes. The mtDNA COI gene of the 12 B. tabaci populations from different regions and countries were cloned and analyzed.Based on mtDNA COI sequences, their biotypes were characterized and phylogenetic relationships among these populations were established by using UPGMA method. The results showed that the genetic similarities among those populations from Beijing, Zhengzhou, Zaozhuang, Nanjing, Shanghai, Haikou,China and the B-biotype populations from California、Texas、Arizona,USA, reached 99.8%-100%, which indicated that most of the B.tabaci populations causing whitefly calamities in China recently attributed to B-biotypes. While only one of the populations collected from Kunming City, Yunnan Province showed very high similarity to Q-biotype B.tabaci from Spain and Morocco.This is the first report on the invasion of Q-biotype B.tabaci into China.
    Synthesis and Identification of Artificial Antigen Against Quinclorac
    Scientia Agricultura Sinica. 2005, 38(01):  86-90 . 
    Abstract ( 1190 )   PDF (367KB) ( 1008 )   Save
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    In this paper, two haptens of quinclorac(Q), 6-[(3,7-dichloro-8-quinolino-formacyl) amino-hexanoic acid (QC) and 4-[(3,7-dichloro-8-quinolino-formamino)] butanoic acid (QB), were synthesized by using quinclorac and amino-hexanoic acid or amino-butanoic acid as original materials. These two haptens and quinclorac were separately conjugated to bovine serum albumin (BSA) with the modified active ester method. Three coating antigens, Q-OVA, QC-OVA and QB-OVA were also synthesized with the mixed-anhydride method. As a result of the study, three types of antibody (Q-Ab, QC-Ab and QB-Ab) were produced successfully. Their titers were 2×103,1.2×106 and 1.6×106 respectively, while the concentration of coating antigens were 4 ?g·ml-1. Among these antibodies, the QB-Ab was utilized satisfactorily with coating antigen Q-OVA to detect the herbicide with heterogenous assays; and its IC50 was 0.4 mg·L-1, the limit of detection(LOD) was 0.006 mg·L-1. The difference of the titer between the homologous and heterologous combinations was significant in this study. It was indicated that the spacer length of the coating antigen is important for the recognition by antibodies, and the sensitivity of ELISA can be improved remarkably by heterologous reaction.
    SOIL & FERTILIZER·WATER-SAVING IRRIGATION
    The Distribution Characteristics of Microbial Biomass Nitrogen in Different Soil Aggregate in Semi-arid Area
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    Scientia Agricultura Sinica. 2005, 38(01):  91-95 . 
    Abstract ( 1312 )   PDF (354KB) ( 1005 )   Save
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    Seven soils were collected from forestland, grassland and cropland in semi-arid area of Inner Mongolia. The soil aggregates of >5 mm, 5-2 mm, 2-1mm, 1-0.5 mm, 0.5-0.25 mm and <0.25 mm were obtained with a dry-sieving method. The total N (TN), microbial biomass N (MBN), K2SO4-extractable N (K2SO4-N) of the soil aggregates were separately determined. The results showed that the different inputs of organic materials and nitrogen fertilizer of the sampled soils significantly affect not only the contents of total N, microbial biomass N and K2SO4-extractable N, but also their distributions in soil aggregates. The total N, microbial biomass N and K2SO4-extractable N were in the ranges of 0.67-2.06 g·kg-1, 11.40-71.03 mg·kg-1and 1.28-4.60 mg·kg-1, respectively. The irrigated soil (No. 6) had the highest value, but the lowest value was in the grassland soil (No.7). Generally, the small aggregates contained significantly lower total N and microbial biomass N than the larger aggregates in the soils with low inputs of organic materials and N fertilizer. In contrast, the small aggregates contained much higher total N and microbial biomass N than the larger aggregates in the soils with high input. It is implied that, under natural condition, N was firstly cycled in the large aggregates. Thus, the N of the large aggregates may play much more important role in natural low nutrition condition than that of the small aggregates.
    Effects of Root Uptake Function and Soil Water on NO3- -N and NH4+-N Distribution
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    Scientia Agricultura Sinica. 2005, 38(01):  96-101 . 
    Abstract ( 984 )   PDF (322KB) ( 1442 )   Save
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    The effects of root uptake and soil water on transfer and distribution of NO3--N in arable soil were studied by using summer maize (Zea mays L. var. Shandan9) as experimental crop. Results showed that root development and water supply had an obvious effect on the transfer and distribution of NO3--N. Under a natural growing conditions with irrigation , the difference of NO3--N concentrations at different distances from the stem was smaller, but obvious difference of NO3--N concentration was observed under the conditions of a limited root growth space without irrigation. Whether restricting root growth space or not, the content of soil NO3--N decreased gradually from 10 cm to 0 cm from the stem, being opposite to the root absorbing area in soils. When root growth space was limited, changes of NO3--N concentration at different distances from the stem were similar to that of soil water content. Results showed that NO3--N could be transferred as solute to plant root systems with water uptake by plants. However, the transfer and distribution of NH4+-N were not influenced by root growth and soil water supply, being different to NO3--N.
    Study on Adsorption and Desorption Properties of Nano-kaoline to Nitrogen, Phosphorus, Potash and Organic Carbon
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    Scientia Agricultura Sinica. 2005, 38(01):  102-109 . 
    Abstract ( 907 )   PDF (530KB) ( 997 )   Save
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    This paper deals with the adsorption and desorption characteristics of kaoline to organic carbon and three nutrient elements, i.e. nitrogen, phosphorus and potash. The adsorption amounts of nano-kaoline to organic carbon , nitrogen, phosphorus and potash were higher than natural kaoline; especially, the adsorption amounts of nano-kaoline to nitrogen, phosphorus, patosh, and organic carbon were 1.5-1.7, 1.9-2.2 , 1.4-2.0, and 1.3-1.9 times higher than that of natural kaoline, respectively. The desorption amounts of natural kaoline and nano-kaoline increased with the increase of the adsorption amounts of organic carbon, nitrogen, phosphorus and potash. Both natural and nano-kaoline adsorption properties could be depicted with Langmuir and Freundlich equations.
    Phosphorus Nutrient Characteristics of Different Maize Inbreds with Tolerance to Low-P Stress
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    Scientia Agricultura Sinica. 2005, 38(01):  110-115 . 
    Abstract ( 933 )   PDF (298KB) ( 613 )   Save
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    Nutrient characteristics of phosphorus in different maize inbred lines with tolerance to low-P stress were studied at stages of seedling, jointing ,earing, silking in a pot experiment. In the periods of seedling and jointing P uptake efficiency is the main contributor to P tolerance, and the relative P content in 99180 and 99239 (both are P-tolerant genotypes) are higher than that in 99152 (P-sensitive genotype). At earing stage P-resistant genotypes, compared to P-sensitive ones, have higher accumulation of P in upper leaves. At silking stage, P uptake and reallocation efficiency of P-tolerant genotypes are higher than those in 99152. The results also suggested that there are different mechanisms of P nutrient characteristics even in different P-tolerant genotypes. Inbred line 99239, according to the investigation, is considered as an efficient stock in the P-uptake while 99180 falls to the efficient stock of P reallocation.
    Influence of Silicon on Cadmium Availability and Cadmium Uptake by Maize in Cadmium-contaminated Soil
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    Scientia Agricultura Sinica. 2005, 38(01):  116-121 . 
    Abstract ( 1024 )   PDF (354KB) ( 958 )   Save
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    Pot experiments were conducted to investigate the influence of silicon on soil cadmium fractionation and cadmium uptake by maize (Zea may L. Nongda 108). The results showed that Cd toxicity in maize treated with Cd at 0.1 or 1.0 mmol·kg-1 (added as CdSO4·8/3H2O) was significantly alleviated by addition of 2.0 g·kg-1 sodium metasilicate (NaSiO3·9H2O) due to the decreased Cd uptake by maize. The ameliorative effect of Si was due to lower exchangeable Cd and Fe/Mn oxide-bound Cd, and higher carbonate-bound and residual Cd in Si-treated Cd-contaminated soils compared with non-Si-treated ones. One of the possible mechanisms of Si-enhancement of resistance to Cd toxicity is that silicate alters the Cd forms in soil, resulting in reduction of Cd bio-availability.
    HORTICULTURE
    Male-Sterile Plants Induced by Anti-gene CYP86MF in Brassica oleracea var. italica
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    Scientia Agricultura Sinica. 2005, 38(01):  122-127 . 
    Abstract ( 1215 )   PDF (520KB) ( 830 )   Save
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    An anti-gene CYP86MF was introduced into hypocotyls of broccoli(Brassica oleracea L.var. italica Plenck) with Agrobacterium tumefaciens, and transgenic plants were obtained by kanamycin selection. It was proved that anti-CYP86MF was integrated with chromosome of transgenic plant, according to the results of PCR, Southern blot, Northern blot. Plants with hypogenetic stamina or ungerminated pollen were observed. The transgenic male-sterile plants could set seeds if artificially pollinated with normal pollen, so the pistil of male-sterile plant was normal, and its sterility was induced by anti-CYP86MF.
    Influence of Heat Stress on the Activity of Protein Kinase in Grape Leaves
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    Scientia Agricultura Sinica. 2005, 38(01):  128-134 . 
    Abstract ( 973 )   PDF (492KB) ( 947 )   Save
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    Abstract: One-year-old grape plants (Vitis vinifera L. cv. Jingxiu) by cuttage were used as experimental materials. The activity of protein kinase in grape leaves after treatment of heat stress (40℃) was measured through substrate phosphorylation in vitro. The results showed that the activity of protein kinase in leaves after heat stress reached a peak value(7 678.7 cpm) as MBP concentration was 0.5 mg·ml-1, and then the activity of protein kinase decreased slightly as MBP concentration increased continuously. The activity of protein kinase exhibited a maximum value as Mg2+ concentration was 5 mmol·L-1 in reaction system with MBP substrate, and then, the activity of protein kinase decreased. Stimulation by Ca2+ to the activity of protein kinase was extremely weak. Mn2+ had no stimulative effect on the activity of protein kinase. Phosphorylation of protein kinase to histone-Ⅲ was weak in reaction system with histone-Ⅲ substrate. Mg2+ and Ca2+ displayed extremely weak effect on kinase activity in the reaction system with histone-Ⅲ substrate. The activity of protein kinase reached a peak value (7 900.9 cpm) as ATP concentration was 50μmol·L-1, then, the activity of protein kinase decreased gradually as ATP concentration increased continuously .
    Isolating Cold-Regulated Genes from Euonymus japonicus 'Zhuzi' Seedlings through Suppression Subtractive Hybridization
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    Scientia Agricultura Sinica. 2005, 38(01):  135-139 . 
    Abstract ( 1075 )   PDF (336KB) ( 927 )   Save
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    Suppression subtractive hybridization (SSH) was utilized for the isolation of cDNA fragments for Euonymus japonicus 'Zhuzi' differentially expressed genes, and forward suppression subtractive cDNA Library of cold-regulated gene was constructed. The seedlings of Euonymus japonicus 'Zhuzi' were treated with low temperature as tester and untreated seedlings as driver. Subtractive cDNA Library was differently screened through cDNA macroarray, six hundreds and four cDNA clones were identified as cold specifically induced or highly expressed. After sequencing of 84 cDNA clones and removing redundant cDNAs, 36 cold-regulated unique cDNA clones were obtained. The results showed that 12 cDNA clones are expected to be novel genes, because no sequence homology with any known sequences was found in GenBank databases. This indicated that it is possible to use SSH and cDNA macroarray in the isolation of cold-regulated genes.
    Study on Purifying Technology of Andrographolide by Supercritical Carbon Dioxide Secondary Gradient Crystallization
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    Scientia Agricultura Sinica. 2005, 38(01):  140-144 . 
    Abstract ( 903 )   PDF (337KB) ( 637 )   Save
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    The effect of secondary gradient crystallizing pressure, temperature and time on the purity and the crystallization ratio of andrographolide was investigated via single factor experiments. The shape of crystal was observed with scanning electron microscopy (SEM). The purity of andrographolide was determined by high pressure liquid chromatography (HPLC). The result showed that the optimum parameters were pressure 14 MPa, temperature 55℃, time 60 min, and under these conditions the particle of andrographolide was much smaller, the crystal of andrographolide was distributed in floccule on crystal board, the purity of andrographolide was 92.5%, the crystallization ratio was 48.9%.
    Establishment of Monoclonal Antibody-Based Immunohistochemistry Assay for Detection of Bovine Spongiform Encephalopathy (BSE) and It's Application
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    Scientia Agricultura Sinica. 2005, 38(01):  145-150 . 
    Abstract ( 1250 )   PDF (281KB) ( 824 )   Save
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    One hybridoma cell line 4C11 secreting specific antibodies to core part of PrPSc in Bovine Spongiform Encephalopathy (BSE) were established from several fusions between mouse myeloma Sp2/0 and spleen cells from mice immunized with recombinant Chinese Luxi yellow cattle PrPc mature protein. An immunohistochemistry assay based on monoclonal antibody 4C11 for the detection of BSE was established and has been applied for the long-term surveillance of BSE in China. By the end of 2002, 3 344 samples from the whole country had been detected and all had negetive results.
    Changes of Dipeptidyl Peptidase Ⅳ in the Processing of Jinhua Ham
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    Scientia Agricultura Sinica. 2005, 38(01):  151-156 . 
    Abstract ( 893 )   PDF (419KB) ( 658 )   Save
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    Sixty experimental Jinhua hams were processed according to typically traditional processing technology with fresh hams from hind legs of local hybrid pigs of Lanxi, Zhejiang Province, China. Biceps femoris was sampled from 5 hams randomly taken each time from one of the 6 processing stage, namely, green ham before salting, end of salting, end of sun-drying, mid-aging, end of aging and end of post-aging. Sample potential DPPⅣ activity at pH 7.0 and temperature 37℃ as well as some general physical and chemical indices were determined. The effects of temperature, sodium chloride content, sodium nitrate content and pH value on muscle DPPⅣ activity were evaluated with Response Surface Method by Design-experts 6.0 software, and muscle actual DPPⅣ activity after each processing stage of Jinhua ham was predicted with response surface regression equation. The results showed that during Jinhua ham processing, processing temperature, biceps femoris pH value and sodium chloride content increased gradually while biceps femoris potential DPPⅣ activity decreased gradually. The actual DPPⅣ activity was significantly influenced by processing temperature, muscle pH value and sodium chloride content (P < 0.05). Though muscle DPPⅣ could keep rather high potential activity during Jinhua ham processing, its actual activity was always very low and only 3.12% of the potential activity could be expressed at most, which therefore indicated that DPPⅣ's role in the formation of Jinhua ham flavor was quite possibly limited.
    Study on the Anti-Oxidative Effect and Mechanism of Polysaccharide from Rapeseed(RSPS)
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    Scientia Agricultura Sinica. 2005, 38(01):  157-162 . 
    Abstract ( 1322 )   PDF (300KB) ( 947 )   Save
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    The anti-oxidative effect and mechanism of RSPS were studied. The K3[Fe(CN)6], TCA system and the crude lipoxygenase were used to evaluate RSPS's capacity of deoxidizing and its inhibition on lipoxygenase. The reagent kits were used to analyse the content of malondialdehyde(MDA) and reactive oxygen species (ROS), and the swelling of mice liver mitochondria was observed in the spectrophotometric method. The results showed that RSPS was a good reducer, and the anti- ROS unit of 2.00 mg·ml-1 RSPS was 94.03, and the inhibiting rate of 2.00 mg·ml-1 RSPS on lipoxygenase was 22.8% in some chemically modified systems. In vitro, 2.00 mg·ml-1 RSPS made the radical-induced swelling of mice liver mitochondria be lower than the swelling of mice liver mitochondria without inducement. The inhibiting rate of 2.00 mg·ml-1 RSPS on radical-induced MDA formation was 30.3% in mice liver mitochondria, while 54.7%(incubation), 32.0%(incubation with Fe2+) and 84.5%(incubation with H2O2) in liver homogenate, respectively. The anti- ROS unit of mice serum, added with 10.00 mg·ml-1 RSPS, was 1340.13. In vivo, there was a significant difference(P<0.01) between the controlled group and the intra- peritoneal injected group (with RSPS 400mg·kg-1 bw·d-1, 12 d) in MDA value in mice liver homogenate. All the results above showed that RSPS has anti-oxidation effect in vitro and in vivo, the high capacity of deoxidising and inhibiting the activity of enzymes associated with oxidation maybe the possible mechanism of RSPS to inhibit oxidation.
    CROP PRODUCTION SYSTEM
    Innovation of the Seed Production Procedures and Establishment of Corresponding Seed Quality Criteria in China
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    Scientia Agricultura Sinica. 2005, 38(01):  163-170 . 
    Abstract ( 1419 )   PDF (246KB) ( 983 )   Save
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    China is one of the largest country in seed production and consumption in the world, but the reformation of seed industry is far lagged behind other sectors of its agricultural economy, thus systematic and complete seed industry reform in China would be worthwhile. In this paper, the innovation of seed production procedures and establishment of the corresponding seed quality criteria system after China entering into WTO is suggested. By gradually abandon three-nursery system, the extension of four-stage seed production procedures should be considered. The system of the "plant-line cycle method" and low temperature and low humidity preservation of the breeder seed is introduced. The establishment of the new seed quality criteria system should meet the requirements of four-stage procedures in order to meet the international standards. In addition, the paper suggested tentatively the schemes of the seed quality criteria for several crops.
    ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT
    Immunohistochemical Localization of Inhibin α Subunit in Hypothalamus and Pituitary of Rat
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    Scientia Agricultura Sinica. 2005, 38(01):  171-175 . 
    Abstract ( 1055 )   PDF (533KB) ( 687 )   Save
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    Using the Strept Avidin Biotin-peroxidase Complex(SABC) immunohistochemical staining technique, immuno- histochemical localization of inhibin α subunit of rat hypothalamus and pituitary with inhibin α subunit monoclonal antibody was performed at different times post the injection. The sexually matured ovari-ectomized rats were injected with inhibin α subunit crude abstracts and different levels of inhibin α subunit fragments. The results showed that positive reaction sites in dose and time dependant ways were detected in the hypothalamus and pituitary, suggesting that inhibin α subunit could pass through the blood-brain barrier arriving at hypothalamus and pituitary. It provided new morphological basis for the research field of a passage mechanism of the subunit through blood-brain barrier.
    Effect of Heat Stress on Apoptosis in Chicken Spleen and Bursa Cells and Their Regulation
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    Scientia Agricultura Sinica. 2005, 38(01):  176-180 . 
    Abstract ( 1129 )   PDF (284KB) ( 726 )   Save
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    Eighteen AA chicken were raised at 22℃, and at 30-day-old of age they were stressed for 0 , 5 and 10 h at 41℃. The DNA contents of spleen and bursa, the ratio of apoptosis and the expression of caspase-3 and Bcl-2 in the spleen and bursa were measured by flow cytometry. The results were as follows: Cell ratio of S phase (DNA synthetic phase ) in the spleen was 4.67%,5.11% and 4.78% , that of the bursa was 30.52%,24.71% and 24.04% at 0,5 and 10 h after heat stress respectively. Apoptotic rate of spleen was 0.22%, 0.42% , 0.32% and that of the bursa was 0.32%,0.62% and 0.41%, respectively. The positive ratios of the cells expressing caspase-3 in the spleen was 5.49%, 10.36% and 9.80%; and that in the bursa was 8.60%, 10.51% and 9.41% respectively. The positive ratio of the cells expressing Bcl-2 protein in the spleens was 31.25%, 24.01% and 29.26%, and that of the bursa was 30.24%, 27.10% and 31.54%, respectively. The apoptotic rate of spleens of heat stressed chickens for 5 h was significantly different from those for 0 and 10 h, separately (P<0.05. After heat stress, apoptotic rate and positive expression of caspase-3 in spleen and bursa increased gradually from 0 to 5 h, and then decreased slowly . The changes of apoptotic rate were parallel positively with the changes of caspase-3 expression, but negatively with the changes of Bcl-2 expression in the spleens and bursa during heat stress. It is concluded that the apoptosis of spleen and bursa cells was obviously affected by heat stress and regulated by the expression of caspase-3 and Bcl-2 protein.
    Studies on Life Cycle and Pathogenicity of Eimeria nocens in Domestic Goslings
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    Scientia Agricultura Sinica. 2005, 38(01):  181-184 . 
    Abstract ( 1090 )   PDF (266KB) ( 765 )   Save
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    Sixteen 10-day-old artificially-reared coccidia-free goslings were inoculated orally with 1.0×105-1.0×106 sporulated oocysts of Eimeria nocens,respectively, and killed at intervals from 30 h to 336 h after inoculations. The life cycle and pathogenicity of E. nocens were observed chronologically. The results showed that at least 3 generations of meronts developed in the endogenous stage of the life cycle of E. nocens. Two types of meronts were found. The first completed maturation at 54 to 78 h after inoculation. These meronts were the first generation meronts, each forming about 10 merozoites. The second completed maturation at 102 to 240 h after inoculation. These meronts were the second or third generation meronts, each forming about 25 merozoites. Development of gametes began at about 198 h after infection. The prepatent period was 9 d and discharge of oocysts continued for 4 d. Sporulation of oocysts occurred in 60-72 h at 25℃. E. nocens invaded posterior jejunum, ileum, ceca, rectum and cloaca. Developmental stages were localized within the epithelial cells of villi and crypts and in lamina propria. Marked histopathological changes including desquamation and necrosis of intestinal epithelium, submucosal edema, hemorrhages, infiltration of inflammatory cells and villous atrophy presented during the periods of late merogony, gamogony and oocyst shedding, but most pronounced in the ileum and the regions adjacent to it. The infected goslings showed severe diarrhoea with necrotic epithelium and blood in the feces, anorexia, emaciation and even deaths, suggesting that E. nocens is highly pathogenic for goslings.
    Effect of Environmental Lead and Cadmium Pollution on Animal Health
    Scientia Agricultura Sinica. 2005, 38(01):  185-190 . 
    Abstract ( 1194 )   PDF (258KB) ( 1125 )   Save
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    The concentrations of twelve minerals (Pb, Cd, Cu, Zn, Fe, As, F, Mo, Se, Mn, Ca, P) in soils, water, forages, crops, grains and blood, hair and tissues of animals were measured in surrounding of the Baiyin mining area. The function and construction of tissues were studied in affected animals. The result indicated that the waste gas and waste water produced in metals melting in factories caused Pb, Cd, Cu and Zn pollution to some extent in the surrounding environment. The concentrations of these elements were significantly higher as compared with those in the normal area (P<0.01). The contents of Pb, Cd were 9 and 680 times in forages, respectively, 10 and 35 times in grain as compared with the control area. The anemia occurred in affected animals belongs to a hypochromic and microcytic pattern. Pathological changes were characterized by the dysfunction and structural lesion in parenchymatous organs. It is therefore suggested that the disease of livestock in this region is caused by lead and cadmium poisoning, mainly due to environment heavy metal pollution by industry activities. Meanwhile, accumulations of Pb and Cd in animal food would impair man's health. Thus it can be seen that knowledge of Pb and Cd concentrations in livestock is important for assessing the effects of pollutions on domestic animals and
    RESEARCH NOTES
    Effects of Soil Texture on Activities of Key Enzymes of Starch Synthesis in Kernel of Winter Wheat Cultivars with Different Gluten Content
    Scientia Agricultura Sinica. 2005, 38(01):  191-196 . 
    Abstract ( 1123 )   PDF (311KB) ( 711 )   Save
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    Three key enzymes AGPP, SSS and SBE associated with starch synthesis were studied during grain filling of three winter wheat cultivars with high,medium and low gluten content in the clay,loam and sandy soil texture in the condition of pond culture. The results indicated that three enzymes activities of high gluten content cultivars were higher in the sandy soil,AGPP activity came to be a double-peak curve,its peaks appeared at 10 d and 20 d after anthesis,respectively. SSS and SBE activities had a single-peak curve,its peak appeared at 20 d after anthesis. It showed that sandy soil could be helpeful to high gluten content cultivars in synthetise starch. AGPP and SSS activities of medium gluten content cultivars showed "sand>loam>clay",but SBE activities of clay and loam soil were higher than that of sandy soil. It showed that three kinds of soil texture could be helpeful medium gluten content cultivars to synthesize starch. Three enzymes activities of low gluten content cultivars were higher in the clay soil,its changing trend came to be a single-peak curve,the peak appeared in 20 d after anthesis. It showed that clay soil could be helpeful low to gluten content cultivars to synthesize starch.
    Effects of Calcium and Calmodulin Antagonist on Antioxidant Systems of Eggplant Seedlings Under High Temperature Stress
    Scientia Agricultura Sinica. 2005, 38(01):  197-202 . 
    Abstract ( 1028 )   PDF (583KB) ( 1122 )   Save
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    After being enhanced or blocked Ca2+-CaM signal transduction by pretreating the eggplants of 'Nongyouqie' and 'Erminqie' with external Ca2+ and calmodulin antagonists TFP and W7, production rate of O2-, the content of MDA, activities of SOD and POD and the contents of Pro and GSH under high temperature stress of 40℃(day/night)were investigated. The results showed that seeds were pretreated with calmodulin antagonist, TFP and W7 resulted in more rapid loss of SOD and POD activity, higher contents of MDA, Pro, and production rate of O2-, in addition, aggravate the damage of GSH; On the contrary, Ca2+ treatment enabled the seedlings to keep relatively high activities of SOD and POD, lower production rate of O2-and MDA content, at the same time, alleviated the damage of GSH and the accumulation of Pro caused by high temperature stress. These results indicated that Ca2+-CaM signal transduction might regulate the heat resistance of eggplant seedlings by affecting the activity of some antioxidant enzymes and the content of antioxidant substance.
    Effects of Defoliation on Endongenous Hormones and Active Oxygen Metabolism of Sweet Cherry Flower Buds During Dormancy
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    Scientia Agricultura Sinica. 2005, 38(01):  203-207 . 
    Abstract ( 1154 )   PDF (330KB) ( 947 )   Save
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    Effects of picking off leaves on endogenous hormones, H2O2, O2- content, SOD, POD, CAT activities in 8-year-old sweet cherry flower buds during dormancy were investigated, and the effect of picking off leaves on dormancy release was also studied. Results indicated that four kinds of endogenous hormones contents in sweet cherry leaves all dropped after picking off leaves, especially ABA content. GA3 and zeatin content increased steadily during upper stage of picking off leaves and were remarkably higher than the control. Meanwhile, H2O2 content in flower buds increased after picking off leaves, it increased by 34.57% compared with the control after picking off leaves for 30 days. However, O2- ccontent decreased by 25.49% compared with the control. Moreover, SOD activity increased and CAT activity decreased after picking off leaves, which were consistent with the reduce of H2O2 content. POD activity increased after picking off leaves. At last, flower buds after picking off leaves germinated 3-6 days earlier than the control, and released dormancy 2-4 days earlier than the control.
    Method and Its Effect of Rapid Introduction of HMW-GS Genes with Good Baking Properties into High-Yielding Wheat Line
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    Scientia Agricultura Sinica. 2005, 38(01):  208-212 . 
    Abstract ( 1360 )   PDF (304KB) ( 706 )   Save
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    In order to introduce HMW-GS genes with good quality properties into high-yielding wheat line 1718 from wheat line Soissons, backcross breeding, PCR-based approach and SDS-PAGE method had been used in wheat segregating progenies grown in field and greenhouse. The results showed that the heredity of the Dx5 gene in random progeny of BC1, BC2, BC3, BC3F1 completely accorded with hereditary rule of a pair of genes and its proportion was 1:1. The plants with Dx5 genes in BC2, BC3, BC3F1, BC3F2 were detected with PCR-based approach, backcrossed and self-inbred after other agronomic characters had been selected. The lines with Dx5 genes were found in BC3F2 progeny by methods as in BC3F2. The morphological characters of line1718-4 were similar to those of the recurrent parent 1718 and also were uniform. 1718-4-6 and 1718-4-10 which carried HMW-GS 1, 7+8, 5+10 were selected from line1718-4 by using SDS-PAGE. Two new lines still retained high-yielding character of their recurrent parent 1718. Their most quality properties were better than that of 1718. The combination of PCR-based approach, SDS-PAGE method with backcross breeding is proposed as a directional, quick, efficient procedure for converging many good HMW-GS genes onto higher yielding lines and improving their genotypes with good quality properties.
    Studies on the Methods of Detecting PVYN and PVYO by RT-PCR
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    Scientia Agricultura Sinica. 2005, 38(01):  213-216 . 
    Abstract ( 1350 )   PDF (265KB) ( 867 )   Save
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    Potato virus Y(PVY) is one of the important viruses infecting potatoes. It influences the production seriously. The nucleotide sequences of potato virus Y N-strain(PVYN)and O-strain(PVYO) were compared. A pair of homologous primers were designed according to the conserved region of coat protein gene. So the RT-PCR method of detecting PVY was built. Judging by the result of comparison, the 3′primer was designed in conserved region and two 5′primers were designed in two variable region. So a complex RT-PCR method was built which could distinguish PVYN and PVYO. The two methods were very powerful for detecting virus-free seed potatoes, investigating PVY and studying its pathology.