Scientia Agricultura Sinica ›› 2005, Vol. 38 ›› Issue (12): 2555-2565 .doi: 10.3864/j.issn.0578-1752.at-2004-3029

• RESEARCH NOTES • Previous Articles     Next Articles

Genetic Diversity Among Barley Germplasm with Known Origins Based on the RAMP and ISSR Markers

,,,,   

  1. 四川农业大学小麦研究所
  • Received:2004-12-16 Revised:1900-01-01 Online:2005-12-10 Published:2005-12-10

PDF

1308

Cited

31

Abstract: The genetic diversity and genetic relationships among 60 barley accessions, including 33 Chinese endemic barley landraces, 19 wild barley accessions from Xizang, China, and 8 barley cultivars from other countries, were evaluated by RAMP and ISSR markers. In RAMP analysis, a total of 116 bands were observed in 22 markers, among with 98 bands were polymorphic. Each primer could amplify 1 to 10 polymorphic bands, with an average of 5.27 bands. The percentage of polymorphic bands (PPB), mean polymorphism information content (PIC) and effective number of alleles (Ne) were 84.48%, 0.277 and 1.602, respectively. Among the 60 barley accessions, the RAMP-based genetic similarity (RAMP-GS) ranged from 0.551 to 0.965, with a mean of 0.830. In ISSR analysis, a total of 113 bands were detected, among which 111 bands were polymorphic. The number of bands from each ISSR marker ranged from 1 to 10, with an average of 5.95. The PPB, PIC and Ne were 98.23%, 0.427 and 2.033, respectively. The ISSR-derived genetic similarity (ISSR-GS) ranged from 0.203 to 0.931, with a mean of 0.676. The cluster analysis indicated that all the 60 barley accessions could be distinguished by both RAMP and ISSR markers. Mantel test indicated that RAMP and ISSR were significantly associated. These results suggested that high level of genetic diversity among the barley germplasm has been detected by RAMP and ISSR markers, and ISSR was superior to RAMP. Key words: Barley; RAMP; ISSR; Genetic diversity; Genetic relationshi

Key words: Barley, RAMP, ISSR, Genetic diversity, Genetic relationship, Cluster analysis

[1] HE ZhiLin, SUN CuiXia, YUE HongLi, TAN YueXia, ZHANG YaoHai, WANG FuSheng, LIU SiTao, JIANG Dong. Genetic Diversity Analysis and GWAS of Alloocimene Based on Resequencing of Citron, Lemon Germplasm Resources [J]. Scientia Agricultura Sinica, 2026, 59(2): 386-401.
[2] XIONG JiaNi, LI ZongYue, HU HengLiang, GU TianYu, GAO Yan, PENG JiaShi. Influence of Expressing OsNRAMP5 Under the Driving of the OsLCT1 Promoter on Cadmium Migration to Rice Seeds [J]. Scientia Agricultura Sinica, 2025, 58(7): 1259-1268.
[3] TONG ZhaoYang, LIU WenHua, ZHANG GuoXin, DONG ChunYan, ZHANG YanXia, XU XiaoWei, HE Dong, LIU HeChun, LI Yang, WANG FengTao, FENG Jing, YAO XiaoBo, LIU MeiJin, LIN RuiMing. The Relationship Between Occurrence of Hulless Barley Ear Rot and Population Migration of Grass Mite (Siteroptes spp.) [J]. Scientia Agricultura Sinica, 2025, 58(3): 493-506.
[4] XU YuJuan, ZHANG Jie, WANG TianYi, CHEN HaoYang, ZHAO JiaJia, WU BangBang, HAO YuQiong, LI XiaoHua, ZHENG XingWei, ZUO JingJing, ZHENG Jun. Identification of Glu-A3 and Glu-B3 of Low-Molecular-Weight Glutenin in Shanxi Wheat and Its Effect on Quality [J]. Scientia Agricultura Sinica, 2025, 58(24): 5110-5127.
[5] CHEN CaiJin, MA Lin, JIANG QingXue, LIU JinHui, MIAO Tong, ZHANG ZhiPeng, MENG Xiang, MA XiaoRan, ZHOU XinYue, ZHANG Jian, LIU WenHui, WANG XueMin. Genetic Diversity Analysis of Phenotypic Traits of 244 Forage Oat Germplasm Resources [J]. Scientia Agricultura Sinica, 2025, 58(23): 4825-4836.
[6] WEI YiMin, ZHOU MeiLiang, TANG Yu. Origin, Evolution and Spread of Crop Buckwheat [J]. Scientia Agricultura Sinica, 2025, 58(21): 4305-4316.
[7] LIU XiaoXu, ZHONG ZeXin, QIU JiaRen, YANG ChunXiao, ZHANG YongJun, XIE Wen, ZHANG YouJun, PAN HuiPeng. GENETIC DIVERSITY OF MTCO1 IN DIFFERENT GEOGRAPHICAL POPULATIONS OF MEGALUROTHRIPS USITATUS [J]. Scientia Agricultura Sinica, 2025, 58(21): 4361-4371.
[8] GUO MengZe, ZHANG Lei, SUN PingPing, JIANG Biao, YAN JinQiang, LI ZhengNan. Molecular Characterization and Evolutionary Dynamics of Tomato Leaf Curl New Delhi Virus Isolate from Wax Gourd (Benincasa hispida) in Guangdong [J]. Scientia Agricultura Sinica, 2025, 58(19): 3890-3904.
[9] LIU PengPeng, LI JiangBo, XU HongJun, NIE YingBin, HAN XinNian, KONG DeZhen, SANG Wei. Genetic Diversity Analysis of Protein Fractions and Quality in Xinjiang Winter Wheat Cultivar Resources [J]. Scientia Agricultura Sinica, 2025, 58(15): 2948-2959.
[10] YANG WenJuan, GAO JiaCheng, WANG YanTing, LI Yan, GUO Ming, WANG JunCheng, MENG YaXiong, WANG HuaJun, SI ErJing. Function of Effector Pg00778 Regulation on the Pathogenicity of Pyrenophora graminea to Barley [J]. Scientia Agricultura Sinica, 2025, 58(15): 3020-3035.
[11] WANG Hui, DING BaoPeng, LI YuXian, REN QuanRu, ZHOU Hai, ZHAO JunLiang, HU HaiFei. Research Progress and Prospects on Crop Pan-Genomics [J]. Scientia Agricultura Sinica, 2025, 58(11): 2045-2061.
[12] LI Pei, HE ZhiLin, TAN YueXia, ZHAO WanTong, FENG JinYing, CHEN GuiHu, YAN Chi, WANG ZiHao, HUANG Ping, JIANG Dong. Genetic Diversity Analysis of Mandarin and Excellent Germplasm Screening Based on Whole-Genome Resequencing Data and Phenotypic Traits [J]. Scientia Agricultura Sinica, 2024, 57(23): 4761-4773.
[13] ZHANG AiHong, YANG Fei, ZHAO YuanYe, ZHAO YiHan, DI DianPing, MIAO HongQin. Pathogenicity and Epidemic Risk of Barley Yellow Striate Mosaic Virus [J]. Scientia Agricultura Sinica, 2024, 57(23): 4686-4697.
[14] XU JinQing, BIAN HaiYan, CHEN TongRui, WANG Lei, WANG HanDong, YOU En, DENG Chao, TANG YouLin, SHEN YuHu. Comparison of the Genome Sequence Polymorphisms Between the Main Naked Barley Varieties Kunlun 14 and Kunlun 15 in Qinghai Province [J]. Scientia Agricultura Sinica, 2024, 57(21): 4192-4204.
[15] YANG Chun, YANG DaiXing, LI Yan, LIANG SiHui, DENG XiaoQiang, QIAO DaHe, CHEN Juan, GUO Yan, LIN KaiQin, CHEN ZhengWu. Comprehensive Analysis of Morphologic Characters and Biochemical Components of Guizhou Dashu Tea Germplasms [J]. Scientia Agricultura Sinica, 2024, 57(19): 3894-3916.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd