Scientia Agricultura Sinica ›› 2026, Vol. 59 ›› Issue (6): 1302-1316.doi: 10.3864/j.issn.0578-1752.2026.06.012

• HORTICULTURE • Previous Articles     Next Articles

Evaluation of Curd Texture Quality in Loose-Curd Cauliflower Germplasm

CHEN MinDong(), QIU BoYin, HUANG Hao, LI YongPing, WEN QingFang()   

  1. Crops Research Institute, Fujian Academy of Agricultural Sciences/Fujian Key Laboratory of Vegetable Genetics and Breeding/ Fujian Engineering Research Center for Vegetables, Fuzhou 350013
  • Received:2025-10-13 Accepted:2026-01-10 Online:2026-03-16 Published:2026-03-24
  • Contact: WEN QingFang

Abstract:

【Objective】By analyzing the curd texture trait data of loose-curd cauliflower germplasm resources, a unified and objective evaluation method for loose-curd cauliflower curd texture was established. The potential factors affecting curd texture were also studied to provide references for the evaluation of loose-curd cauliflower edible quality and quality breeding. 【Method】Texture Profile Analysis (TPA) and sensory evaluation method were used to evaluate 166 loose-curd cauliflower germplasm resources. Data of 5 TPA indicators (hardness, cohesiveness, springiness, chewiness, resilience) and 3 sensory evaluation indicators (sensory crispness, sensory hardness, sensory chewiness) were obtained. Correlation analysis and linear regression analysis were carried out to construct the relationship model between sensory evaluation indicators and texture parameters. Through principal component analysis, membership function analysis and cluster analysis, a mathematical model for loose-curd cauliflower curd texture evaluation was established, and the texture groups of loose-curd cauliflower germplasm resources were divided. Furthermore, the differences in cell microstructure and cell wall component contents of loose-curd cauliflower curds among different groups were analyzed to explore the potential factors affecting loose-curd cauliflower curd texture. 【Result】Significant differences were observed in eight curd texture indicators among different loose-curd cauliflower germplasms, with chewiness showing the greatest degree of dispersion. Sensory evaluation indicators were positively correlated with TPA indicators to varying extents. By establishing linear regression equations between them, it was found that hardness has important predictive value in sensory texture evaluation. Principal Component Analysis (PCA) extracted two principal components affecting loose-curd cauliflower curd texture, with a cumulative contribution rate of 90.22%. Among them, the first principal component mainly includes hardness, chewiness, and springiness, which are key parameters determining the palatability of loose-curd cauliflower. A mathematical model for evaluating loose-curd cauliflower curd texture was established: Comprehensive texture score C=-2.045+0.017×chewiness+3.108×resilience+0.555×springiness+0.010×hardness+1.950×cohesiveness. Based on the C values, 166 loose-curd cauliflower germplasms were classified into three groups. GroupⅠhad the lowest C values (0.01≤C≤0.35), characterized by low hardness and poor chewiness, and was classified as the soft type; GroupⅡ had moderate C values (0.37≤C≤0.62), with balanced texture and good palatability, and was classified as the crisp-tender type; GroupⅢhad the highest C values (0.65≤C≤1.00), exhibiting high hardness and chewiness, and was classified as the crisp-springy type. Significant differences in cell structure and cell wall components were detected among cauliflower florets of different textures. The crisp-springy type featured florets with high cell roundness, compact structure, and high contents of cellulose and hemicellulose; the crisp-tender type had florets with plump and uniformly structured cells, and an appropriate ratio of cellulose, hemicellulose, and soluble pectin contents; the soft type displayed florets with obvious cell gaps and loose structure, along with a higher soluble pectin content. 【Conclusion】A mathematical model for loose-curd cauliflower curd texture evaluation was established: C=-2.045+0.017×chewiness+3.108×resilience+0.555×springiness+0.010×hardness+1.950×cohesiveness. The C value ranges dividing different loose-curd cauliflower curd texture types can be used for the identification of loose-curd cauliflower germplasm resources. It was also found that cell structure and cell wall components have important effects on the texture quality of loose-curd cauliflower curds.

Key words: loose-curd cauliflower, germplasm resources, curd texture evaluation, texture profile analysis (TPA), sensory evaluation method

Table 1

Sensory evaluation standard of loose-curd cauliflower"

指标
Index
指标描述
Index description
评价标准Evaluation standard
0—4分0-4 points 5—7分5-7 points 8—10分8-10 points
感官脆度
Sensory crispness
样品出现折断时的力
The force at which the sample breaks
不脆
Not crisp
较脆
Relatively crisp
很脆
Very crisp
感官硬度
Sensory hardness
牙齿挤压样品的力量
The force of teeth squeezing the sample
不硬
Not hard
较硬
Relatively hard
很硬
Very hard
感官咀嚼性
Sensory chewiness
咀嚼固体样品时需要的能量
The energy required for chewing solid sample
咀嚼性差
Poor chewiness
咀嚼性适中
Moderate chewiness
耐咀嚼
Chewy

Table 2

Definition of 5 TPA indexs"

指标Index 指标描述Index description
硬度
Hardness
第一次挤压循环的最大力量峰值,单位N
The maximum peak force of the first squeezing cycle, Unit N
内聚性
Cohesiveness
第二次挤压循环的正峰面积同第一次挤压循环的正峰面积的比值,计算方式为A2/A1
The ratio of the positive peak area of the second extrusion cycle to the positive peak area of the first extrusion cycle. The calculation method is A2/A1
弹性
Springiness
第一挤压结束后第二次挤压开始前样品恢复的高度,计算方式为T2/T1
The height at which the sample recovers after the first compression is completed and before the second compression begins. The calculation method is T2/T1
咀嚼性
Chewiness
咀嚼样品所需的能量,计算方式为内聚性×硬度×弹性,单位mJ
The energy required to chew the sample. The calculation method is cohesiveness×hardness ×springiness, Unit mJ
回复性
Responsiveness
样品在第1次压缩过程中回弹的能力,计算方式为A4/A3
The ability of the sample to rebound during the first compression process. The calculation method is A4/A3

Fig. 1

TPA characteristic curve The meaning of the text in the figure is the same as in Table 2"

Table 3

Variation and variance analysis in sensory evaluation index and TPA index of loose-curd cauliflower curd"

指标Index 最大值Max 最小值Min 平均值Average 标准差SD 变异系数CV (%) FF-value
感官指标
Sensory index
感官脆度 Sensory crispness 7.66 3.29 6.04 0.92 15.22 6.246**
感官硬度 Sensory hardness 7.30 3.70 6.12 0.89 14.60 8.357**
感官咀嚼性 Sensory chewiness 7.80 3.00 6.24 0.97 15.60 7.011**
TPA指标
TPA index
硬度 Hardness 50.82 22.50 35.62 6.20 17.40 46.033**
内聚性 Cohesiveness 0.41 0.27 0.33 0.03 8.60 4.330**
弹性 Springiness 1.46 0.98 1.21 0.12 10.20 24.530**
咀嚼性 Chewiness 26.95 6.47 14.69 4.67 31.80 30.032**
回复性 Responsiveness 0.24 0.16 0.20 0.02 10.08 10.506**

Table 4

Correlation analysis in sensory evaluation index and TPA index"

指标Index 感官脆度Sensory crispness 感官硬度Sensory hardness 感官咀嚼性Sensory chewiness
硬度Hardness 0.438** 0.471** 0.755**
内聚性Cohesiveness 0.135** 0.051** 0.188**
弹性Springiness 0.280** 0.203** 0.366**
咀嚼性Chewiness 0.393** 0.363** 0.637**
回复性Responsiveness 0.227** 0.295** 0.520**

Table 5

Multiple linear regression analysis of sensory evaluation index and TPA index"

感官指标Sensoryindex 预测模型Predictionmodel R2 PP value
感官脆度Sensory crispness Y=2.825+0.091X1 0.279 <0.001
感官硬度Sensory hardness Y=3.112+0.085X1 0.258 <0.001
感官咀嚼性Sensory chewiness Y=-0.146+0.172X1-0.092X4+8.051X5 0.548 <0.001

Table 6

Correlation analysis between 5 TPA index"

指标Index 硬度Hardness 内聚性Cohesiveness 弹性Springiness 咀嚼性Chewiness 回复性Responsiveness
硬度Hardness 1.000
内聚性Cohesiveness 0.225** 1.000
弹性Springiness 0.536** 0.564** 1.000
咀嚼性Chewiness 0.868** 0.536** 0.807** 1.000
回复性Responsiveness 0.685** 0.500** 0.673** 0.803** 1.000

Table 7

Load matrix, eigenvalues, contribution rate, and cumulative contribution rate of each principal component after rotation"

指标 Index 第一主成分 First principal component 第二主成分 Second principal component
硬度Hardness 0.907 -0.544
内聚性Cohesiveness 0.646 0.713
弹性Springiness 0.862 0.419
咀嚼性Chewiness 0.901 -0.230
回复性Responsiveness 0.813 -0.150
特征值Eigenvalues 3.456 1.055
贡献率Contribution rate (%) 69.12 21.10
累计贡献率Cumulative contribution rate (%) 69.12 90.22

Table 8

Comprehensive score value C of curd texture in 166 loose-curd cauliflower germplasm resources"

编号No. CC value 编号No. CC value 编号No. CC value 编号No. CC value
1 0.02 43 0.96 85 0.27 127 0.74
2 0.05 44 0.33 86 0.41 128 0.69
3 0.47 45 0.26 87 0.77 129 0.82
4 0.12 46 0.71 88 0.80 130 0.89
5 0.40 47 0.43 89 0.24 131 0.55
6 0.20 48 0.92 90 0.27 132 0.76
7 0.06 49 0.22 91 0.33 133 0.81
8 0.42 50 0.52 92 0.37 134 0.40
9 0.55 51 0.55 93 0.46 135 0.97
10 0.39 52 0.33 94 0.61 136 0.98
11 0.77 53 0.60 95 0.38 137 0.85
12 0.46 54 0.46 96 0.27 138 0.43
13 0.45 55 0.73 97 0.26 139 0.62
14 0.39 56 0.39 98 0.55 140 0.73
15 0.44 57 0.62 99 0.75 141 0.76
16 0.32 58 0.28 100 0.48 142 0.69
17 0.30 59 0.87 101 0.31 143 0.73
18 0.12 60 0.66 102 0.33 144 0.87
19 0.20 61 0.53 103 0.62 145 0.78
20 0.27 62 0.79 104 0.57 146 0.69
21 0.09 63 0.53 105 0.49 147 0.80
22 0.47 64 0.23 106 0.59 148 0.42
23 0.26 65 0.45 107 0.87 149 0.38
24 0.09 66 0.65 108 0.65 150 0.61
25 0.09 67 0.30 109 0.50 151 0.52
26 0.19 68 0.68 110 0.45 152 0.91
27 0.16 69 0.54 111 0.31 153 0.98
28 0.01 70 0.28 112 0.51 154 0.79
29 0.28 71 0.33 113 0.58 155 0.71
30 0.28 72 0.28 114 0.88 156 0.60
31 0.14 73 0.34 115 0.67 157 0.53
32 0.09 74 0.56 116 0.53 158 0.82
33 0.28 75 0.37 117 0.45 159 0.97
34 0.24 76 0.27 118 0.76 160 0.54
35 0.28 77 0.41 119 0.81 161 0.48
36 0.29 78 0.37 120 0.29 162 0.61
37 0.28 79 0.40 121 0.50 163 0.71
38 0.28 80 0.35 122 0.29 164 0.77
39 0.52 81 0.28 123 0.73 165 0.75
40 0.62 82 0.28 124 0.61 166 0.74
41 0.55 83 0.45 125 0.74
42 0.97 84 0.19 126 1.00

Fig. 2

Curd texture cluster analysis of 166 loose-curd cauliflower germplasms"

Fig. 3

Box plot of texture traits of different groups of loose-curd cauliflower germplasm Different letters indicate significant differences (P<0.05)"

Table 9

Cell morphology of different groups of loose-curd cauliflower germplasm"

类群
Group
编号
No.
长度
Length (μm)
宽度
Width (μm)
面积
Area (μm2)
周长
Circumference (μm)
横纵比
Aspectratio
圆度
Roundness
25 103.10 56.66 6269.00 327.74 1.82 0.73
103 95.23 61.13 5261.29 290.90 1.56 0.77
128 96.01 65.08 5403.58 297.26 1.48 0.78

Fig. 4

Microstructure of flower branch cells in different groups of loose-curd cauliflower germplasm A: Group Ⅰ (Germplasm 25); b: Group Ⅱ (Germplasm 103); c: Group Ⅲ (Germplasm 128). Scale bar: 50 μm; Magnification: 200×"

Fig. 5

Content of cell wall components in different populations of loose-curd cauliflower germplasm"

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