红橘响应褐斑病菌侵染的转录组学分析

doi:10.3864/j.issn.0578-1752.2020.22.006

Abstract

Abstract:

【Objective】The objective of this study is to reveal the gene expression pattern at transcriptional level of tangerine (Citrus reticulata Blanco, cv. Hongjv) after inoculated with the brown spot pathogen (Alternaria alternata tangerine pathotype), and to identify the key genes of tangerine responding to the pathogen infection.【Method】Detached leaves of tangerine were inoculated with A. alternata tangerine pathotype for 28 h and used for RNA extraction and transcriptome sequencing. Sequencing results were verified by quantitative real-time PCR (qRT-PCR). Differentially expressed genes (DEGs) were screened with |log₂fold change|≥1, q-value≤0.01 as threshold and clean reads were compared with genome of sweet orange (Citrus sinensis). GO database was used to perform functional classification of DEGs, KEGG was used to analyze metabolic pathways, and MapMan software was used to analyze the expression changes of genes related to biological stress signaling pathways.【Result】A large number of DEGs related to stress were produced at 28 h post inoculation, 5 173 up-regulated and 6 555 down-regulated genes were obtained. GO functional classification showed that the DEGs were mainly related to protein binding, membrane and oxidation-reduction process. KEGG enrichment and MapMan software analysis revealed that the basic metabolism of tangerine was severely damaged during the stress of A. alternata tangerine pathotype. The expression of multiple genes in host defense-related plant hormone signal transduction pathways such as ethylene (ET), salicylic acid (SA) and auxin (AUX) varied to some extent, of which ET plays a key role. The three members of the ET receptor ETR were activated to different degrees, among which downstream kinases and ET response factors were up-regulated, whereas most key signal genes of AUX, most members of AUX response factor ARF and genes in SA synthesis pathway were all down-regulated. Meanwhile, genes related to flavonol, anthocyanins, terpenoids and alkaloid synthesis were drastically changed by the fungus. Most genes of terpenoid synthesis were down-regulated, while the number and expression trend of up-regulated genes related to flavonoid synthesis were stronger than those of the down-regulated genes. Glucosinolate genes with anti-insect and antibacterial effects were up-regulated. Furthermore, a large number of transcription factors involved in stress resistance processes such as WRKY, bZIP, ERF, MYB and NAC were induced and activated. Most members of WRKY and bZIP transcription factors were positively regulated by the fungus, and the expressions of more than 50% of the ERF family genes were up-regulated. Under the regulation of transcription factors, PTI and ETI response genes such as receptor kinases, R proteins and NBS anti-disease proteins were largely expressed, multiple resistance PR genes were up-regulated and the expressions of 22 POD genes of antioxidant protective enzyme systems were largely activated by reactive oxygen species. The above results indicated that A. alternata tangerine pathotype infection had a great effect on the physiological state of citrus plants. To validate the findings, 19 DEGs related to anti-disease were selected for further analyses using qRT-PCR. The result was fairly consistent with that of transcriptional sequencing.【Conclusion】The DEGs and the significantly up-regulated expression genes obtained in C. reticulata Blanco, cv. Hongjv were mainly involved in the metabolic process, the response to stress and the transcriptional regulation. The synergistic regulation of these genes is an important mechanism of defense reaction to A. alternata tangerine pathotype.

Key words: Citrus reticulata Blanco, cv. Hongjv, Alternaria alternata tangerine pathotype, transcriptome, differentially expressed gene (DEG)

TANG KeZhi,ZHOU ChangYong. Transcriptome Analysis of Citrus reticulata Blanco, cv. Hongjv Infected with Alternaria alternata Tangerine Pathotype[J].Scientia Agricultura Sinica, 2020, 53(22): 4584-4600.

Figures/Tables 12

Table 1

Table 2

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Fig. 7

Fig. 8

Fig. 9

Fig. 10

References 54

[1]	KOHMOTO K, SCHEFFER R P, WHITESIDE J O . Host-selective toxins from Alternaria citri. Phytopathology, 1979,69(6):667-671. doi: 10.1094/Phyto-69-667
[2]	NISHIMURA S, KOHMOTO K . Host-specific toxins and chemical structures from Alternaria species. Annual Review of Phytopathology, 1983,21:87-116. doi: 10.1146/annurev.py.21.090183.000511 pmid: 25946338
[3]	李红叶, 梅秀凤, 符雨诗, 黄峰, 陈再廖, 杨小平, 王一光, 黄海华 . 柑橘链格孢褐斑病的发生危害风险和治理对策. 果树学报, 2015,32(5):969-976.
	LI H Y, MEI X F, FU Y S, HUANG F, CHEN Z L, YANG X P, WANG Y G, HUANG H H . Alternaria brown spot of citrus: The risk and management strategy. Journal of Fruit Science, 2015,32(5):969-976. (in Chinese)
[4]	COBB N . Letters on the diseases of plants- Alternaria of the citrus tribe. Agricultural Gazette of New South Wales, 1903,14:955-986.
[5]	WANG X F, LI Z A, TANG K Z, ZHOU C Y, YI L . First report of Alternaria brown spot of citrus caused by Alternaria alternata in Yunnan Province, China. Plant Disease, 2010,94(3):375. doi: 10.1094/PDIS-94-3-0375B pmid: 30754225
[6]	陈昌胜, 黄峰, 程兰, 冯春刚, 黄涛江, 李红叶 . 红橘褐斑病病原鉴定. 植物病理学报, 2011,41(5):449-455.
	CHEN C S, HUANG F, CHENG L, FENG C G, HUANG T J, LI H Y . Identification of the pathogenic fungus causing brown spot on tangerine ( Citrus reticulate cv. Hongjv). Acta Phytopathologica Sinica, 2011,41(5):449-455. (in Chinese)
[7]	黄峰, 朱丽, 侯欣, 李红叶 . 瓯柑褐斑病病原鉴定. 浙江农业科学, 2012(9):1281-1282.
	HUANG F, ZHU L, HOU X, LI H Y . Identification of the pathogenic fungus causing brown spot onCitrus suavissima Hort. ex Tanaka. Zhejiang Agricultural Sciences, 2012(9):1281-1282. (in Chinese)
[8]	赵圆, 王玲杰, 王雪峰, 唐科志, 周常勇, 李太盛 . 杂柑褐斑病的病原鉴定. 果树学报, 2014,31(2):292-295.
	ZHAO Y, WANG L J, WANG X F, TANG K Z, ZHOU C Y, LI T S . Identification of the pathogenic fungus causing brown spot in two tangerine hybrid varieties. Journal of Fruit Science, 2014,31(2):292-295. (in Chinese)
[9]	CHUNG K R . Mitogen-activated protein kinase signaling pathways of the tangerine pathotype of Alternaria alternate. MAP Kinase, 2013,2(4):16-23.
[10]	YANG S L, YU P L, CHUNG K R . The glutathione peroxidase- mediated reactive oxygen species resistance, fungicide sensitivity and cell wall construction in the citrus fungal pathogen Alternaria alternate. Environmental Microbiology, 2016,18(3):923-935. doi: 10.1111/1462-2920.13125 pmid: 26567914
[11]	唐飞艳, 孔向雯, 吕韦玮, 唐科志 . 柑橘褐斑病菌AaSIP2基因生物学功能初步研究. 园艺学报, 2018,45(12):2358-2370.
	TANG F Y, KONG X W, LÜ W W, TANG K Z . Preliminary studies on the function of AaSIP2 gene in the tangerine pathotype of Alternaria alternata. Acta Horticulturae Sinica, 2018,45(12):2358-2370. (in Chinese)
[12]	MA H J, ZHANG B, GAI Y P, SUN X P, CHUNG K R , LI H Y. Cell-wall-degrading enzymes required for virulence in the host selective toxin-producing necrotroph Alternaria alternata of citrus. Frontiers in Microbiology, 2019, 10: Article 2514.
[13]	WANG M S, SUN X P, YU D L, XU J P, CHUNG K R, LI H Y . Genomic and transcriptomic analyses of the tangerine pathotype of Alternaria alternata in response to oxidative stress. Scientific Reports, 2016,6:32437. doi: 10.1038/srep32437 pmid: 27582273
[14]	王明爽 . 柑橘褐斑病菌比较基因组和转录组分析及柑橘绿霉病菌产孢中心调控途径和高渗甘油途径的功能基因研究[D]. 杭州: 浙江大学, 2016.
	WANG M S . Comparative genome and transcriptome analysis of the tangerine pathotype of Alternaria alternate and functional analysis of genes involved in the central regulatory pathway of conidiation and the high osmolarity glycerol pathway in Penicillium digitatum[D]. Hangzhou: Zhejiang University, 2016. (in Chinese)
[15]	WU J, ZHANG Y L, ZHANG H Q, HUANG H, FOLTA K M, LU J . Whole genome wide expression profiles of Vitis amurensis grape responding to downy mildew by using Solexa sequencing technology. BMC Plant Biology, 2010,10:234. doi: 10.1186/1471-2229-10-234 pmid: 21029438
[16]	XU L, ZHU L F, TU L L, LIU L L, YUAN D J, LIN L, LONG L, ZHANG X L . Lignin metabolism has a central role in the resistance of cotton to the wilt fungus Verticillium dahliae as revealed by RNA-Seq-dependent transcriptional analysis and histochemistry. Journal of Experimental Botany, 2011,62(15):5607-5621. doi: 10.1093/jxb/err245
[17]	WARD J A, WEBER C A . Comparative RNA-seq for the investigation of resistance to Phytophthora root rot in the red raspberry ‘Latham’. Acta Horticulturae, 2012,946(946):67-72.
[18]	WANG Y S, ZHOU L J, YU X Y, STOVER E, LUO F , DUAN Y P. Transcriptome profiling of Huanglongbing ( HLB) tolerant and susceptible Citrus plants reveals the role of basal resistance in HLB tolerance. Frontiers in Plant Science, 2016, 7: Article 933.
[19]	李湘龙, 柏斌, 吴俊, 邓启云, 周波 . 第二代测序技术用于水稻和稻瘟菌互作早期转录组的分析. 遗传, 2012,34(1):102-112.
	LI X L, BAI B, WU J, DENG Q Y, ZHOU B . Transcriptome analysis of early interaction between rice and Magnaporthe oryzae using next-generation sequencing technology. Hereditas, 2012,34(1):102-112. (in Chinese)
[20]	KIM D, LANGMEAD B, SALZBERG S L . HISAT: A fast spliced aligner with low memory requirements. Nature Methods, 2015,12(4):357-360. doi: 10.1038/nmeth.3317 pmid: 25751142
[21]	MATSUKAWA M, SHIBATA Y, OHTSU M, MIZUTANI A, MORI H, WANG P, OJIKA M, KAWAKITA K, TAKEMOTO D . Nicotiana benthamiana calreticulin 3a is required for the ethylene-mediated production of phytoalexins and disease resistance against oomycete pathoge. Phytophthora infestans. Molecular Plant-Microbe Interactions, 2013,26(8):880-892.
[22]	GUNDLACH H , MÜLLER M J, KUTCHAN T M, ZENK M H. Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. Proceedings of the National Academy of Sciences of the United States of America, 1992,89(6):2389-2393. doi: 10.1073/pnas.89.6.2389 pmid: 11607285
[23]	NOJIRI H, SUGIMORI M, YAMANE H, NISHIMURA Y, YAMADA A, SHIBUYA N, KODAMA O, MUROFUSHI N, OMORI T . Involvement of jasmonic acid in elicitor-induced phytoalexin production in suspension-cultured rice cells. Plant Physiology, 1996,110(2):387-392. doi: 10.1104/pp.110.2.387 pmid: 12226190
[24]	VAN WEES S C M, CHANG H S, ZHU T, GLAZEBROOK J . Characterization of the early response of Arabidopsis t. Alternaria brassicicola infection using expression profiling. Plant Physiology, 2003,132(2):606-617. doi: 10.1104/pp.103.022186 pmid: 12805591
[25]	VAHABI K, REICHELT M, SCHOLZ S S, FURCH A C U, MATSUO M, JOHNSON J M, SHERAMETI I, GERSHENZON J , OELMULLER R. Alternaria brassicae induces systemic jasmonate responses in Arabidopsis which travel to neighboring plants via a Piriformsopora indica hyphal network and activate abscisic acid responses. Frontiers in Plant Science, 2018, 9: Article 626.
[26]	DESMOND O J, EDGAR C I, MANNERS J M, MACLEAN D J, SCHENK P M, KAZAN K . Methyl jasmonate induced gene expression in wheat delays symptom development by the crown rot pathogen Fusarium pseudograminearum. Physiological and Molecular Plant Pathology, 2006,67:171-179. doi: 10.1016/j.pmpp.2005.12.007
[27]	JAYARAJ J, MUTHUKRISHNAN S, LIANG G H, VELAZHAHAN R . Jasmonic acid and salicylic acid induce accumulation of β-1,3-glucanase and thaumatin-like proteins in wheat and enhance resistance against Stagonospora nodorum. Biologia Plantarum, 2004,48(3):425-430. doi: 10.1023/B:BIOP.0000041097.03177.2d
[28]	PANDEY S P, SOMSSICH I E . The role of WRKY transcription factors in plant immunity. Plant Physiology, 2009,150(4):1648-1655. doi: 10.1104/pp.109.138990 pmid: 19420325
[29]	LI J, BRADER G, PALVA E T . The WRKY70 transcription factor: A node of convergence for jasmonate-mediated and salicylate-mediated signals in plant defense. The Plant Cell, 2004,16(2):319-331. doi: 10.1105/tpc.016980 pmid: 14742872
[30]	ABUQAMAR S, CHEN X, DHAWAN R, BLUHM B, SALMERON J, LAM S, DIETRICH R A, MENGISTE T . Expression profiling and mutant analysis reveals complex regulatory networks involved in Arabidopsis response t. Botrytis infection. The Plant Journal, 2006,48(1):28-44. doi: 10.1111/j.1365-313X.2006.02849.x pmid: 16925600
[31]	ZHEN Z Y, QAMAR S A, CHEN Z X, MENGISTE T . Arabidopsis WRKY33 transcription factor is required for resistance to necrotrophic fungal pathogens. The Plant Journal, 2006,48(4):592-605. doi: 10.1111/j.1365-313X.2006.02901.x pmid: 17059405
[32]	ANDREASSON E, JENKINS T, BRODERSEN P, THORGRIMSEN S, PETERSEN N H, ZHU S, QIU J L, MICHEELSEN P, ROCHER A, PETERSEN M, NEWMAN M A, NIELSEN H B, HIRT H, SOMSSICH I, MATTSSON O, MUNDY J . The map kinase substrate MKS1 is a regulator of plant defense responses. The EMBO Journal, 2005,24(14):2579-2589. doi: 10.1038/sj.emboj.7600737 pmid: 15990873
[33]	QIU J L, FIIL B K, PETERSEN K, NIELSEN H B, BOTANGA C J, THORGRIMSEN S, PALMA K , SUAREZ-RODRIGUEZ M C, SANDBECH-CLAUSEN S, LICHOTA J, BRODERSEN P, GRASSER K D, MATTSSON O, GLAZEBROOK J, MUNDY J, PETERSEN M. Arabidopsis map kinase 4 regulates gene expression through transcription factor release in the nucleus. The EMBO Journal, 2008,27(16):2214-2221. doi: 10.1038/emboj.2008.147 pmid: 18650934
[34]	LIU Y, XIN J, LIU L, SONG A, GUAN Z, FANG W, CHEN F . A temporal gene expression map of chrysanthemum leaves infected with Alternaria alternata reveals different stages of defense mechanisms. Horticulture Research, 2020,7:23. doi: 10.1038/s41438-020-0245-0 pmid: 32140232
[35]	FAN Q, SONG A, XIN J, CHEN S, JIANG J, WANG Y, LI X, CHEN F . Cmwrky15 facilitate. Alternaria tenuissima infection of chrysanthemum. PLoS ONE, 2015,10(11):e0143349.
[36]	PRE M, ATALLAH M, CHAMPION A, DE VOS M, PIETERSE C M, MEMELINK J . The AP2/ERF domain transcription factor ORA59 integrates jasmonic acid and ethylene signals in plant defense. Plant Physiology, 2008,147(3):1347-1357. doi: 10.1104/pp.108.117523 pmid: 18467450
[37]	PIETERSE C M, LEON-REYES A , VAN DER ENT S, VAN WEES S C. Networking by small-molecule hormones in plant immunity. Nature Chemical Biology, 2009,5(5):308-316. doi: 10.1038/nchembio.164 pmid: 19377457
[38]	GUTTERSON N, REUBER T L . Regulation of disease resistance pathways by AP2/ERF transcription factors. Current Opinion in Plant Biology, 2004,7(4):465-471. doi: 10.1016/j.pbi.2004.04.007
[39]	YU W Q, ZHAO R R, SHENG J P, SHEN L . SLERF2 is associated with methyl jasmonate-mediated defense response agains. Botrytis cinerea in tomato fruit. Journal of Agricultural and Food Chemistry, 2018,66(38):9923-9932.
[40]	BERROCAL-LOBO M, MOLINA A . Ethylene response factor 1 mediates Arabidopsis resistance to the soilborne fungu. Fusarium oxysporum. Molecular Plant-Microbe Interactions, 2004,17(7):763-770. doi: 10.1094/MPMI.2004.17.7.763 pmid: 15242170
[41]	BERROCAL-LOBO M, MOLINA A, SOLANO R . Constitutive expression of ETHYLENE-RESPONSE-FACTOR1 i. Arabidopsis confers resistance to several necrotrophic fungi. The Plant Journal, 2002,29(1):23-32. doi: 10.1046/j.1365-313x.2002.01191.x pmid: 12060224
[42]	LEON-REYES A, DU Y J, KOORNNEEF A, PROIETTI S, KORBES A P, MEMELINK J, PIETERSE C M, RITSEMA T . Ethylene signaling renders the jasmonate response of Arabidopsis insensitive to future suppression by salicylic acid. Molecular Plant-Microbe Interactions, 2010,23(2):187-197. doi: 10.1094/MPMI-23-2-0187 pmid: 20064062
[43]	FERRER J L, AUSTIN M B, STEWART Jr . C, NOEL J P. Structure and function of enzymes involved in the biosynthesis of phenylpropanoids. Plant Physiology and Biochemistry, 2008,46(3):356-370. doi: 10.1016/j.plaphy.2007.12.009
[44]	ZHU L, NI W, LIU S, CAI B, XING H , WANG S. Transcriptomics analysis of apple leaves in response to Alternaria alternata apple pathotype infection. Frontiers in Plant Science, 2017, 8: Article 22.
[45]	DORIA M S, GUEDES M S , DE ANDRADE SILVA E M, DE OLIVEIRA T M, PIROVANI C P, KUPPER K C, BASTIANEL M, MICHELI F. Comparative proteomics of two citrus varieties in response to infection by the fungus Alternaria alternata. International Journal of Biological Macromolecules, 2019,136:410-423. doi: 10.1016/j.ijbiomac.2019.06.069 pmid: 31199975
[46]	SUN H H, WANG L, ZHANG B Q, MA J H, HETTENHAUSEN C, CAO G Y, SUN G L, WU J Q, WU J S . Scopoletin is a phytoalexin against Alternaria alternata in wild tobacco dependent on jasmonate signalling. Journal of Experimental Botany, 2014,65(15):4305-4315. doi: 10.1093/jxb/eru203
[47]	THOLL D . Biosynthesis and biological functions of terpenoids in plants. Advances in Biochemical Engineering/Biotechnology, 2015,148:63-106. doi: 10.1007/10_2014_295 pmid: 25583224
[48]	SONG N, MA L, WANG W, SUN H, WANG L, BALDWIN I T, WU J S . An ERF2-like transcription factor regulates production of the defense sesquiterpene capsidiol upon Alternaria alternata infection. Journal of Experimental Botany, 2019,70(20):5895-5908.
[49]	CHEN X, CHEN H, YUAN J S, KOLLNER T G, CHEN Y, GUO Y, ZHUANG X, CHEN X, ZHANG Y J, FU J , NEBENFÜHR A, GUO Z, CHEN F. The rice terpene synthase gene OsTPS19 functions as an( S)-limonene synthase in planta, and its overexpression leads to enhanced resistance to the blast fungus Magnaporthe oryzae.Plant Biotechnology Journal 2018, 16(10):1778-1787.
[50]	HAMMERBACHER A, COUTINHO T A, GERSHENZON J . Roles of plant volatiles in defense against microbial pathogens and microbial exploitation of volatiles. Plant, Cell and Environment, 2019,42(10):2827-2843. doi: 10.1111/pce.13602 pmid: 31222757
[51]	HUANG H, ULLAH F, ZHOU D X, YI M , ZHAO Y. Mechanisms of ROS regulation of plant development and stress responses. Frontiers in Plant Science, 2019, 10: Article 800.
[52]	MARINO D, DUNAND C, PUPPO A, PAULY N . A burst of plant NADPH oxidases. Trends in Plant Science, 2012,17(1):9-15. doi: 10.1016/j.tplants.2011.10.001
[53]	TORRES M A . ROS in biotic interactions. Physiologia Plantarum, 2010,138(4):414-429. doi: 10.1111/j.1399-3054.2009.01326.x pmid: 20002601
[54]	KUŹNIAK E . The ascorbate-gluathione cycle and related redox signals in plant pathogen interactions//ANJUM N A. Ascorbate- Glutathione Pathway and Stress Tolerance in Plants. Springer Science+Business Media B. V, 2010: 115-136.
	责任编辑: 岳梅

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

基因ID Gene ID	引物序列 Primer sequence (5′-3′)		产物长度 Length of product (bp)
Cs4g12760	F: TTCGTCTTGCTCTTCGGATAA	R: GCACTCCAACGGAATCTCTAA	202
Cs2g09310	F: GGTGTCATTCCTCCTCCTACC	R: GCAGTTCCCTCGCCTATTCT	198
Cs5g15470	F: CAGCCTTGTCGGTATGAGAA	R: CAACACCCAATCTTCCTTGAG	151
Cs5g21860	F: GCTCTTGTGGGCATTCTTGC	R: CTCTCGTGTAGAAGCTCTTGCC	142
Cs7g20700	F: ACGGTTCAGGCTCATTTCAG	R: TGGGATTTGGCATCATCAAT	185
Cs3g10430	F: TGGAGACGTAGAGGCTGTCAA	R: CATACCAATATTTTGAGCATTTT	121
Cs6g07410	F: GGAGAGTGGTGGAATGCTGAT	R: CACTTCGAGCGTGTAGGTTTG	150
Cs6g20850	F: CCAGCAGGCATGAGAAATTA	R: TGACCATCGTGGGAACAGTA	229
Cs1g01180	F: TGCAGTAGAAGTTGATGGTGATG	R: AATGAGCCGTTAGCGACAAG	165
Cs1g04680	F: AGCTGCAAGGGTTTGGTTAG	R: GAATTTGCGTTTGGTGATGA	150
Cs8g13680	F: GCCTATGCTTGCTGTTGTTTC	R: GAAGGCAGTCCATCCATACTTC	194
orange1.1t03118	F: CAAGCTCTCCAGGCAAGAGT	R: GGTCCACGGCCATAGTAGAA	247
orange1.1t03618	F: CGGGATGAACATTTGGTTTA	R: CTAGCCTTCTGATCTTGACACA	184
orange1.1t05311	F: GCTGGGATATAACTCCTTCTCA	R: TTCCGCTAAACCAATCACTT	172
Cs2g06120	F: GCACAAGGAAATGGGTTTGT	R: GAAACACGCTGGGATCACTT	229
Cs6g07400	F: ACATGGCTGCAAGAGCATAC	R: CCATTGAGGTCCACCACTTA	197
orange1.1t00214	F: ACGCTCTGTCCCTCAACAAG	R: CCGCTACTGCCTCCTGTATC	171
orange1.1t02319	F: GGGATCTACTGCCGACACTC	R: CGACGACGACCTTTGATCTT	245
orange1.1t03603	F: GGACAATGCTGATCCGAAAG	R: TCAACCAAGCCTCCTGAAAC	203
CitActin	F: CATCCCTCAGCACCTTCC	R: CCAACCTTAGCACTTCTCC	191

样本 Sample	总序列数 Total reads	比对上序列比例 Total mapped reads (%)	比对上唯一位置序列比例 Unique mapped reads (%)	双端比对上序列比例 Reads mapped in paired (%)
C0_1	47713764	90.30	86.99	84.18
C0_2	54696950	88.96	85.64	82.20
C0_3	52284118	89.93	86.59	83.36
ZC_1	55857454	75.22	71.84	69.94
ZC_2	53372222	74.18	70.98	68.37
ZC_3	57202046	74.31	70.99	68.87

Transcriptome Analysis of Citrus reticulata Blanco, cv. Hongjv Infected with Alternaria alternata Tangerine Pathotype

RichHTML

PDF

Abstract

Cite this article

share this article

Figures/Tables 12

References 54

Related Articles 15

Metrics

Comments

Recommended 0

[1]	WANG ZhongNi, LEI Yue, LI JiaLi, GONG YanLong, ZHU SuSong. Functions of ABC Transporter OsARG1 in Rice Heading Date Regulation [J]. Scientia Agricultura Sinica, 2026, 59(1): 1-16.
[2]	WANG SiQi, ZOU LiRen, BAI RuiWen, YAN Ke, WANG SiYang, QI XiaoGuang, SHEN HaiLin, WEN JingHui. Screening of Key Genes Related to Gibberellic Acid Regulation of Rachis Hardening in Honey Grapes [J]. Scientia Agricultura Sinica, 2026, 59(1): 179-189.
[3]	ZOU XiaoWei, XIA Lei, ZHU XiaoMin, SUN Hui, ZHOU Qi, QI Ji, ZHANG YaFeng, ZHENG Yan, JIANG ZhaoYuan. Analysis of Disease Resistance Induced by Ustilago maydis Strain with Overexpressed UM01240 Based on Transcriptome Sequencing [J]. Scientia Agricultura Sinica, 2025, 58(6): 1116-1130.
[4]	SUN Ping, ZHU WenCan, LIN XianRui, WU JiaQi, CAO YiWen, CHEN ChenFei, WANG Yi, ZHU JianXi, JIA HuiJuan, QIAN MinJie, SHEN JianSheng. Effects of Rainy and Low Light Conditions on Coloration and Flavonoid Accumulation in Peach Peel Based on Metabolomic and Transcriptomic Analyses [J]. Scientia Agricultura Sinica, 2025, 58(6): 1173-1194.
[5]	XIE LuLu, LI Fu, ZHANG SiYuan, GAO JianChang. Analysis of Conserved Genes in Adventitious Root Formation Based on Cross Species Transcriptomes [J]. Scientia Agricultura Sinica, 2025, 58(6): 1195-1209.
[6]	GAO YanHao, WANG TingTing, BAI WeiWei, DU XingJie, LIU Xian, QIN BenYuan, FU Tong, SUN Yu, GAO TengYun, ZHANG TianLiu. The Combination of Lipidome and Transcriptome Revealed the Differential Expression Patterns of Lipid Characteristics in Different Muscle Tissues for Nanyang Cattle [J]. Scientia Agricultura Sinica, 2025, 58(6): 1239-1258.
[7]	WANG Fan, LIU ChenWei, LU HongChen, XU RenChao, BIAN XiaoChun. Transcriptome Analysis of Vicia faba Response to Alternaria alternata Infection and Validation of the Disease Resistance Function of VfPR4 [J]. Scientia Agricultura Sinica, 2025, 58(22): 4656-4672.
[8]	MU YingTong, LU JingShi, ZHANG YuTong, SHI FengLing. Identification of Key Drought-Responsive Genes in Upright Medicago ruthenica Sojak cv. Zhilixing Based on Transcriptome Sequencing and WGCNA [J]. Scientia Agricultura Sinica, 2025, 58(21): 4528-4543.
[9]	PAN Yuan, WANG De, LIU Nan, MENG XiangLong, DAI PengBo, LI Bo, HU TongLe, WANG ShuTong, CAO KeQiang, WANG YaNan. Evaluation of the Effectiveness of Two High-Throughput Sequencing Techniques in Identifying Apple Viruses and Identification of Two Novel Viruses [J]. Scientia Agricultura Sinica, 2025, 58(2): 266-280.
[10]	LIN Yan, ZHENG LingLing, TIAN Jia, WEN Yue, CHEN Chen, WANG Lei. Based on Transcriptomics and Proteomics to Provide Insights into the Molecular Mechanisms of Calyx Abscission in Korla Xiangli [J]. Scientia Agricultura Sinica, 2025, 58(18): 3744-3765.
[11]	DONG Xue, CHEN MengQiu, SHAO Jin, WU XueYou, TANG PeiAn. Construction of a Differential Gene Expression and Quality Regulation Network in Stored Rice Grain Using WGCNA [J]. Scientia Agricultura Sinica, 2025, 58(14): 2885-2903.
[12]	CAO YanYong, CHENG ZeQiang, MA Juan, YANG WenBo, ZHU WeiHong, SUN XinYan, LI HuiMin, XIA LaiKun, DUAN CanXing. Integrating Transcriptomic and Metabolomic Analyses Reveals Maize Responses to Stalk Rot Caused by Fusarium proliferatum [J]. Scientia Agricultura Sinica, 2025, 58(1): 75-90.
[13]	QI RenJie, NING Yu, LIU Jing, LIU ZhiYang, XU Hai, LUO ZhiDan, CHEN LongZheng. Identification and Analysis of Genes Related to Bitter Gourd Saponin Synthesis Based on Transcriptome Sequencing [J]. Scientia Agricultura Sinica, 2024, 57(9): 1779-1793.
[14]	LIN Wei, WU ShuiJin, LI YueSen. Transcriptome and Proteome Association Analysis to Revealthe Molecular Mechanism of Baxi Banana Seedlings in Response to Low Temperature [J]. Scientia Agricultura Sinica, 2024, 57(8): 1575-1591.
[15]	GAO ChenXi, HAO LuYang, HU Yue, LI YongXiang, ZHANG DengFeng, LI ChunHui, SONG YanChun, SHI YunSu, WANG TianYu, LI Yu, LIU XuYang. Analysis of Transposable Element Associated Epigenetic Regulation under Drought in Maize [J]. Scientia Agricultura Sinica, 2024, 57(6): 1034-1048.