基于转录组测序的苦瓜皂苷合成相关基因的鉴定和分析

doi:10.3864/j.issn.0578-1752.2024.09.012

Abstract

Abstract:

【Objective】As the main source of bitterness in bitter gourd fruit, saponin possesses various medicinal values including hypoglycemic and anticancer properties. This paper aimed at identifying the metabolic pathways and genes involved in the regulation of bitter gourd saponin biosynthesis, so as to provide the theoretical basis for further analysis of the molecular mechanism of bitter gourd saponin formation.【Method】Using the bitter gourd high-generation inbred line GK24 as the test material, fruit tissues were sampled at the ovary stage (T1), young fruit stage (T2), commodity fruit stage (T3), and maturity fruit stage (T4). The saponin content of bitter gourd at different periods were determined by the vanillin-glacial acetic acid method, and the differentially expressed genes (DEGs) were identified using the method of transcriptome sequencing. 【Result】From T1 to T4, the endogenous saponin content of bitter gourd showed significant decrease with fruit development. A total of 17 504 genes were identified by transcriptome sequencing, and the number of down-regulated genes was higher than that of up-regulated genes in the comparison of the three groups of T1-vs-T2, T2-vs-T3, and T3-vs-T4. GO enrichment analysis showed that phosphorus-containing complex metabolic process, kinesin complex and 2-succinyl-6-hydroxy-cyclohexadiene-1-carboxylic acid synthetase activity were the most significantly enriched terms in biological process, cellular component and molecular function, respectively. KEGG analysis showed that global and overview mapping, carbohydrate metabolism, and amino acid metabolism were the most significantly enriched pathways in all three comparative groups. The genes could be categorized into 20 profiles based on their expression patterns. Genes in profile 0 gradually decreased from T1 to T4, showing similar pattern of saponin content changes with bitter gourd fruit development. 14 genes related to bitter gourd saponin biosynthesis were identified from profile 0, including AAT1, HMG1, MVK, PMK, MVD2, and FPS1 in the terpene skeleton biosynthesis pathway, SS12, SQE1, and CPQ in the sesquiterpene and triterpene biosynthesis pathway, and the postmodifying enzyme genes CYP97A3, CYP71AN24, UGT94E5 and UGT73C6. The results of RNA-seq were validated by qRT-PCR method.【Conclusion】The saponin content of bitter gourd decreased with the fruit development. Bitter gourd saponin was synthesized through the terpenoid bone biosynthesis (ko00900) metabolic pathway to accumulate terpenoid saponin skeleton in the first, and then produce saponins through the sesquiterpene and triterpene biosynthesis (ko00909) metabolic pathway and the modification of oxidoreductase and glycosyltransferase successively. A total of 14 genes related to the saponin biosynthesis of bitter gourd have been identified in the present study.

Key words: bitter gourd, transcriptome, differentially expressed genes, saponin, regulation of synthesis

QI RenJie, NING Yu, LIU Jing, LIU ZhiYang, XU Hai, LUO ZhiDan, CHEN LongZheng. Identification and Analysis of Genes Related to Bitter Gourd Saponin Synthesis Based on Transcriptome Sequencing[J].Scientia Agricultura Sinica, 2024, 57(9): 1779-1793.

Figures/Tables 12

Table 1

Fig. 1

Table 2

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Table 3

Table 4

Genes enriched in the ko00900 and ko00909 pathways in profile 0"

基因编号 Gene number	基因注释 Gene function	通路 Pathway	T1 FPKM	T2 FPKM	T3 FPKM	T4 FPKM
evm.TU.chr1.608	法尼基半胱氨酸裂解酶异构体 Farnesylcysteine lyase isoform	ko00900	38.15	28.42	17.24	10.65
evm.TU.chr10.805	2-甲基-D-赤藓糖醇2,4-环二磷酸合酶 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	ko00900	115.94	71.18	43.36	26.32
evm.TU.chr2.34	香叶基香叶基二磷酸还原酶样异构体 Geranylgeranyl diphosphate reductase-like isoform	ko00900	30.58	17.55	16.44	7.16
evm.TU.chr3.1930	羟基-3-甲基丁-2-烯-1-基二磷酸合酶 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase	ko00900	433.17	194.37	106.87	85.58
evm.TU.chr3.2317	法尼基焦磷酸合酶1 Farnesyl pyrophosphate synthase 1	ko00900	358.50	164.00	51.39	7.70
evm.TU.chr4.352	乙酰辅酶A乙酰转移酶 Acetyl-CoA acetyltransferae	ko00900	439.08	95.24	57.25	34.89
evm.TU.chr4.613	4-羟基-3-甲基丁-2-烯基二磷酸还原酶 4-hydroxy-3-methylbut-2-enyl diphosphate reductase	ko00900	1091.57	439.96	370.39	217.45
evm.TU.chr6.140	1-脱氧-D-酮糖5-磷酸还原异构酶 1-deoxy-D-xylulose 5-phosphate reductoisomerase	ko00900	239.29	131.64	82.92	76.05
evm.TU.chr6.518	脱氧-D-酮糖-5-磷酸合成酶Deoxy-D-xylulose-5-phosphate synthase	ko00900	637.07	42.68	19.10	0.17
evm.TU.chr4.378	HMG-CoA合成酶 3-hydroxy-3-methyl glutaryl coenzyme A synthesis	ko00900	1274.61	1010.52	91.31	2.87
evm.TU.chr8.4201	甲羟戊酸激酶 Mevalonate kinase	ko00900	105.59	52.77	31.46	14.07
evm.TU.chr9.3672	焦磷酸甲羟戊酸脱羧酶Pyrophosphomethylvalerate decarboxylase	ko00900	671.59	131.25	53.80	28.02
evm.TU.chr10.2918	磷酸甲羟戊酸激酶 Phosphomevalonate kinase	ko00900	85.10	58.17	9.86	3.75
evm.TU.chr6.363	磷酸甲羟戊酸激酶 Phosphomevalonate kinase	ko00900	41.95	20.49	13.08	12.38
evm.TU.chr2.1021	角鲨烯合成酶 Squalene synthase	ko00909	437.66	285.81	89.01	48.19
evm.TU.chr3.2797	橙花内酯合成酶 Nerolidol synthase	ko00909	15.85	9.29	0.69	0.07
evm.TU.chr7.10	葫芦二烯醇合酶 Cucurbitadienol synthase	ko00909	981.27	444.87	79.12	4.09
evm.TU.chr8.588	角鲨烯环氧酶 Squalene epoxidase 1	ko00909	1030.32	506.37	76.82	1.14

Table 4

Fig. 7

Fig. 8

References 34

[1]	丁雷, 胡玉立, 李梅, 吴丽丽, 秦灵灵, 刘桐华. 苦瓜的药食价值研究概括. 中国医药导报, 2021, 18(13): 117-120.
	DING L, HU Y L, LI M, WU L L, QIN L L, LIU T H. Summary of research on the medicinal and edible value of bitter gourd. China Medical Journal, 2021, 18(13): 117-120. (in Chinese)
[2]	崔宏伟, 韩汶延, 于蕾, 苏秀兰. 苦瓜化学成分及药理作用研究进展. 世界科学技术-中医药现代化, 2021, 23(5): 1712-1719.
	CUI H W, HAN W Y, YU L, SU X L. Research progress on the chemical composition and pharmacological effects of bitter gourd. World Science and Technology-Modernization of Traditional Chinese Medicine, 2021, 23(5): 1712-1719. (in Chinese)
[3]	LIU C, DAI L H, LIU Y P, DOU D Q, SUN Y X, MA L Q. Pharmacological activities of mogrosides. Future Medicinal Chemistry, 2018, 10(8): 845-850. doi: 10.4155/fmc-2017-0255 pmid: 29432030
[4]	李莎莎. 苦瓜皂苷降血糖及促进糖尿病创面愈合作用的研究[D]. 广州: 南方医科大学, 2022.
	LI S S. Study on glucose lowering and diabetic wound healing effects of Momordica charantia L. saponin[D]. Guangzhou: Southern Medical University, 2022. (in Chinese)
[5]	刘馨泽, 吴新民, 蔺冬梅, 冯琳, 杨洪梅, 王欢, 王冬雪, 刘淑莹, 吴巍, 万茜淋. 苦瓜化学成分的提取分离及药理活性研究进展. 中成药, 2022, 44(1): 177-182.
	LIU X Z, WU X M, LIN D M, FENG L, YANG H M, WANG H, WANG D X, LIU S Y, WU W, WAN X L. Research progress on extraction, separation and pharmacological activities of chemical constituents from Momordica charantia L. Chinese Traditional Patent Medicine, 2022, 44(1): 177-182. (in Chinese)
[6]	郑方园. 苦瓜皂苷的分离纯化、结构鉴定及苦瓜颗粒剂工艺研究[D]. 合肥: 安徽农业大学, 2021.
	ZHENG F Y. Separation and purification of Momordica charantia saponins, identification of structure and the technology of Momordica charantia granules[D]. Hefei: Anhui Agricultural University, 2021. (in Chinese)
[7]	KUMBHALKAR B B, RAJOPADHYE A A, UPADHYE A S. Standardization of family Cucurbitaceae. Current Science, 2013, 104(12): 1595-1596.
[8]	OKABE H, MIYAHARA Y, YAMAUCHI T. Studies on the constituents of Momordica charantia L. IV. Characterization of the new cucurbitacin glycosides of the immature fruits. (2). Structures of the bitter glycosides, momordicosides K and L. Chemical and Pharmaceutical Bulletin, 1982, 30(12): 4334-4340. doi: 10.1248/cpb.30.4334
[9]	YASUDA M, IWAMOTO M, OKABE H, YAMAUCHI T. Structures of momordicines I, II and III, the bitter principles in the leaves and vines of Momordica charantia L. Chemical and Pharmaceutical Bulletin, 1984, 32(5): 2044-2047. doi: 10.1248/cpb.32.2044
[10]	安昌, 秦源, 方韵颖, 屈祺, 王炳锐, 郑平. 药用植物三萜类生物合成途径及关键酶基因研究进展. 中国中药杂志, 2022, 47(24): 6560-6572.
	AN C, QIN Y, FANG Y Y, QU Q, WANG B R, ZHENG P. Biosynthetic pathways of triterpenes in medicinal plants and key enzyme genes: A review. China Journal of Chinese Materia Medica, 2022, 47(24): 6560-6572. (in Chinese)
[11]	徐圆圆, 陈仲, 贾黎明, 翁学煌. 植物三萜皂苷生物合成途径及调控机制研究进展. 中国科学: 生命科学, 2021, 51(5): 525-555.
	XU Y Y, CHEN Z, JIA L M, WENG X H. Advances in understanding of the biosynthetic pathway and regulatory mechanism of triterpenoid saponins in plants. Scientia Sinica (Vitae), 2021, 51(5): 525-555. (in Chinese)
[12]	魏一丁, 熊超, 张天缘, 高翰, 尹青岗, 姚辉, 孙伟, 胡志刚, 陈士林. 基于转录组数据对七叶树中三萜皂苷合成途径的研究. 中国中药杂志, 2019, 44(6): 1135-1144.
	WEI Y D, XIONG C, ZHANG T Y, GAO H, YIN Q G, YAO H, SUN W, HU Z G, CHEN S L. Synthesis of triterpenoid saponins from Aesculus chinensis based on transcriptome data. China Journal of Chinese Materia Medica, 2019, 44(6): 1135-1144. (in Chinese)
[13]	ZHU J H, LI H L, GUO D, WANG Y, DAI H F, MEI W L, PENG S Q. Identification, characterization and expression analysis of genes involved in steroidal saponin biosynthesis in Dracaena cambodiana. Journal of Plant Research, 2018, 131(3): 555-562. doi: 10.1007/s10265-017-1004-7
[14]	UPADHYAY S, PHUKAN U J, MISHRA S, SHUKLA R K. De novo leaf and root transcriptome analysis identified novel genes involved in steroidal sapogenin biosynthesis in Asparagus racemosus. BMC Genomics, 2014, 15(1): 746. doi: 10.1186/1471-2164-15-746
[15]	SINGH P, SINGH G, BHANDAWAT A, SINGH G, PARMAR R, SETH R, SHARMA R K. Spatial transcriptome analysis provides insights of key gene(s) involved in steroidal saponin biosynthesis in medicinally important herb Trillium govanianum. Scientific Reports, 2017, 7: 45295. doi: 10.1038/srep45295
[16]	单春苗, 王晨凯, 施圆圆, 张声祥, 赵历强, 吴家文. 多花黄精甾体皂苷生物合成途径分析及关键酶基因研究. 中国中药杂志, 2020, 45(12): 2847-2857.
	SHAN C M, WANG C K, SHI Y Y, ZHANG S X, ZHAO L Q, WU J W. Identification of key enzyme genes involved in biosynthesis of steroidal saponins and analysis of biosynthesis pathway in Polygonatum cyrtonema. China Journal of Chinese Materia Medica, 2020, 45(12): 2847-2857. (in Chinese)
[17]	石博, 万新建, 关峰, 张景云, 黄长林, 张会国, 黄国东. 苦瓜皂苷最佳提取工艺优化及其对α-葡萄糖苷酶的抑制活性. 食品研究与开发, 2023, 44(11): 145-150.
	SHI B, WAN X J, GUAN F, ZHANG J Y, HUANG C L, ZHANG H G, HUANG G D. Optimization of extraction process of saponins from Momordica charantia and their inhibitory activity on α-glucosidase. Food Research and Development, 2023, 44(11): 145-150. (in Chinese)
[18]	徐斌, 董英. 分光光度法测定苦瓜总皂甙含量. 食品科学, 2005, 26(10): 165-169.
	XU B, DONG Y. Determination on total saponins of Momordica charantia L. by spectrophotometry. Food Science, 2005, 26(10): 165-169. (in Chinese)
[19]	MATSUMURA H, HSIAO M C, LIN Y P, TOYODA A, TANIAI N, TARORA K, URASAKI N, ANAND S S, DHILLON N P S, SCHAFLEITNER R, LEE C R. Long-read bitter gourd (Momordica charantia) genome and the genomic architecture of nonclassic domestication. Proceedings of the National Academy of Sciences of the United States of America, 2020, 117(25): 14543-14551.
[20]	薛禹宸, 雷秀娟, 张浩, 王英平. 不同生长时期西洋参花蕾中皂苷成分及最佳采收期的确定. 吉林农业大学学报, 2021, 43(6): 697-704.
	XUE Y C, LEI X J, ZHANG H, WANG Y P. Distribution of saponins in flower buds of American ginseng in different growth periods and determination of the suitable harvesting time. Journal of Jilin Agricultural University, 2021, 43(6): 697-704. (in Chinese)
[21]	苏璐璐, 朱文莹, 陈旭, 王辉, 曹依林, 王富. 基于转录组学和代谢组学解析番茄果实品质形成过程. 山东农业科学, 2023, 55(2): 9-19.
	SU L L, ZHU W Y, CHEN X, WANG H, CAO Y L, WANG F. Analysis of tomato fruit quality forming based on transcriptomics and metabolomics. Shandong Agricultural Sciences, 2023, 55(2): 9-19. (in Chinese)
[22]	王红娟, 唐荣莉, 蒋晓英, 官玲, 雷开荣, 黄启中, 林清. 辣椒不同发育时期果实的转录组分析. 西南农业学报, 2022, 35(3): 517-525.
	WANG H J, TANG R L, JIANG X Y, GUAN L, LEI K R, HUANG Q Z, LIN Q. Transcriptome analysis of pepper fruit at different developmental stages. Southwest China Journal of Agricultural Sciences, 2022, 35(3): 517-525. (in Chinese)
[23]	AUGUSTIN J M, KUZINA V, ANDERSEN S B, BAK S. Molecular activities, biosynthesis and evolution of triterpenoid saponins. Phytochemistry, 2011, 72(6): 435-457. doi: 10.1016/j.phytochem.2011.01.015 pmid: 21333312
[24]	SHANG Y, MA Y S, ZHOU Y, ZHANG H M, DUAN L X, CHEN H M, ZENG J G, ZHOU Q, WANG S H, GU W J, LIU M, REN J W, GU X F, ZHANG S P, WANG Y, YASUKAWA K, BOUWMEESTER H J, QI X Q, ZHANG Z H, LUCAS W J, HUANG S W. Biosynthesis, regulation, and domestication of bitterness in cucumber. Science, 2014, 346(6213): 1084-1088. doi: 10.1126/science.1259215
[25]	LI X J, LIN S J, XIANG C G, LIU W Q, ZHANG X J, WANG C C, LU X H, LIU M S, WANG T, LIU Z X, WANG N N, GAO L H, HAN X, ZHANG W N. CUCUME: An RNA methylation database integrating systemic mRNAs signals, GWAS and QTL genetic regulation and epigenetics in different tissues of Cucurbitaceae. Computational and Structural Biotechnology Journal, 2023, 21: 837-846. doi: 10.1016/j.csbj.2023.01.012 pmid: 36698975
[26]	朱源, 李检秀, 李坚斌, 黄日波. 药用植物中四环三萜皂苷合成生物学研究进展. 广西科学院学报, 2020, 36(3): 309-316.
	ZHU Y, LI J X, LI J B, HUANG R B. Research progress in synthetic biology of tetracyclic triterpenoids saponins in medicinal plants. Journal of Guangxi Academy of Sciences, 2020, 36(3): 309-316. (in Chinese)
[27]	ZHANG J S, DAI L H, YANG J G, LIU C, MEN Y, ZENG Y, CAI Y, ZHU Y M, SUN Y X. Oxidation of cucurbitadienol catalyzed by CYP87D18 in the biosynthesis of mogrosides from Siraita grosvenorii. Plant and Cell Physiology, 2016, 57(5): 1000-1007. doi: 10.1093/pcp/pcw038
[28]	ITKI M, DACIEOVICH R R, COHEN S, PORTONY V, DORON- FAIGENBOIM A, OREN E, FREILICH S, TZURI G, BARANES N, SHEN S, PETERIKOV M, SERTCHOOK R, BEN-DOR S, GOTTLIEB H, HERNANDEZ A, NELSON D R, PARIS H S, TADMOR Y, BURGER Y, LEWINSOHN E, KATZIR N, SCHAAFFER A. The biosynthetic pathway of the nonsugar, high-intensity sweetener mogroside V from Siraita grosvenorii. Proceedings of the National Academy of Sciences of the USA, 2016, 113(47): E7619-E7628.
[29]	季宏与, 丁常宏, 于丹, 都晓伟. 转录组学技术在人参皂苷生物合成途径研究中的应用进展. 食品与发酵工程, 2022, 48(16): 318-325.
	JI H Y, DING C H, YU D, DU X W. Progress in the application of transcriptomics technology in the study of ginsenoside biosynthesis pathways. Food and Fermentation Industry, 2022, 48(16): 318-325. (in Chinese)
[30]	穆事成. 糖基转移酶的筛选及罗汉果苷糖基化修饰[D]. 天津: 天津科技大学, 2021.
	MU S C. The screening of glycosyltransferase and glycosylation modification of mogrosides[D]. Tianjin: Tianjin University of Science and Technology, 2021. (in Chinese)
[31]	OSBOURN A. Secondary metabolic gene clusters: Evolutionary toolkits for chemical innovation. Trends in Genetics, 2010, 26: 449-457. doi: 10.1016/j.tig.2010.07.001 pmid: 20739089
[32]	HAMBERGER B, BAK S. Plant P450s as versatile drivers for evolution of species-specific chemical diversity. Philosophical Transactions of the Royal Society B:Biological Sciences, 2013, 368: 20120426. doi: 10.1098/rstb.2012.0426
[33]	THIMMAPPA R, GEISLER K, LOUVEAU T, O'MAILLE P, OSBOURN A. Triterpene biosynthesis in plants. Annual Review of Plant Biology, 2014, 65: 225-257. doi: 10.1146/annurev-arplant-050312-120229 pmid: 24498976
[34]	KIM O, BANG K, KIM Y C, DONG Y H, MIN Y K, CHA S W. Upregulation of ginsenoside and gene expression related to triterpene biosynthesis in ginseng hairy root cultures elicited by methyl jasmonate. Plant Cell, Tissue and Organ Culture (PCTOC), 2009, 98(1): 25-33. doi: 10.1007/s11240-009-9535-9

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

基因名 Gene name	正向引物Forward primer (5′-3′)	反向引物Reverse primer (5′-3′)
CPQ	GTTGAAAGAGGGGGAAGAGG	CACCAGACCCGGAAGTAAAA
FSP1	GCGGATCTCAGGTCAACATT	TCCAGGCACATTGTAGTCCA
CYP97A3	GGGAGATATGGCGTGTAAGAAGAC	CTAAAGTCAGACGAGAGAACAGGG
CYP71AN24	GAACACAAGGCCAAGATGAGAG	CAGTTGTGTCAGTTCCAGCTAC
UGT73C6	TGCCTCCAGAGCTGAAACAT	TCCCATGAATCCAGCCACTT
UGT94E5	TCATCACACAACCAAAGGCC	ATCGGAATGTTGAGCGATGC
SQE1	AGCAAGGGACGGTTACTTCA	TGAGAAGCAGCCATCACAGA
MVK	GCAGTCGTTCATGGATCCAC	TGGCCACGAAAACTCCAATG
PMK	GGGACCATGAGAGGACTGAG	AGGGTCAGACTTTTGCCACT
Actin	GGCAAACCCTAAAGTTTTCTTCG	GATGAGCCCTTGTAATGAAGTGG

样品 Sample	原始数据 Raw reads	过滤后数据 Clean reads	Q30含量 Q30 content (%)	GC含量 GC content (%)	单一比对率 Unique mapped (%)	总比对率 Total mapped (%)
T1-1	46878594	46673674	92.28	46.80	93.74	96.88
T1-2	55531244	55302940	92.51	46.59	93.74	96.89
T1-3	65068774	64756962	91.96	46.30	93.49	96.61
T2-1	53923956	53678084	91.97	46.70	93.42	96.63
T2-2	46863574	46589694	92.55	47.09	93.61	96.87
T2-3	51548340	51304488	92.54	47.04	93.43	96.74
T3-1	44171240	43942622	92.37	46.84	93.10	95.88
T3-2	44849744	44668862	92.68	47.20	94.17	97.07
T3-3	55212924	54973380	92.20	47.14	93.68	96.43
T4-1	69720780	69450220	92.42	47.30	93.73	96.46
T4-2	66405070	66157562	92.94	46.67	94.16%	96.82
T4-3	64248114	63979034	92.29	46.69	92.75	96.29

通路名称 Pathway name	通路ID Pathway ID	基因数目 Number of genes	所有基因数 Number of all genes	P值 P value
核糖体 Ribosome	ko03010	83	305	3.36E-13
同源重组 Homologous recombination	ko03440	20	58	1.03E-05
DNA复制 DNA replication	ko03030	16	46	7.03E-05
氨基酸的生物合成 Biosynthesis of amino acids	ko01230	44	202	9.41E-05
错配修复 Mismatch repair	ko03430	14	39	0.000135124
缬氨酸、亮氨酸和异亮氨酸生物合成 Valine, leucine and isoleucine biosynthesis	ko00290	8	18	0.000748137
真核生物核糖体的生物合成 Ribosome biogenesis in eukaryotes	ko03008	19	77	0.002133956
嘧啶代谢 Pyrimidine metabolism	ko00240	15	58	0.00377061
2-氧代羧酸代谢 2-Oxocarboxylic acid metabolism	ko01210	14	53	0.004117066
糖鞘脂生物合成 Glycosphingolipid biosynthesis	ko00604	3	4	0.006896205
精氨酸生物合成 Arginine biosynthesis	ko00220	10	35	0.008097428
碱基切除修复 Base excision repair	ko03410	13	52	0.009192653
核质运输 Nucleocytoplasmic transport	ko03013	20	98	0.01563779
赖氨酸生物合成 Lysine biosynthesis	ko00300	5	13	0.01582533
萜类骨架生物合成 Terpenoid backbone biosynthesis	ko00900	14	62	0.0173777
糖胺聚糖降解 Glycosaminoglycan degradation	ko00531	6	18	0.01771733
氨酰基tRNA生物合成 Aminoacyl-tRNA biosynthesis	ko00970	13	57	0.0198342
不同类型的N-聚糖生物合成 Various types of N-glycan biosynthesis	ko00513	8	30	0.02599519
组氨酸代谢 Histidine metabolism	ko00340	5	16	0.03922549

Identification and Analysis of Genes Related to Bitter Gourd Saponin Synthesis Based on Transcriptome Sequencing

RichHTML

PDF

Abstract

Cite this article

share this article

Figures/Tables 12

References 34

Related Articles 15

Metrics

Comments

Recommended 0

[1]	WANG ZhongNi, LEI Yue, LI JiaLi, GONG YanLong, ZHU SuSong. Functions of ABC Transporter OsARG1 in Rice Heading Date Regulation [J]. Scientia Agricultura Sinica, 2026, 59(1): 1-16.
[2]	WANG SiQi, ZOU LiRen, BAI RuiWen, YAN Ke, WANG SiYang, QI XiaoGuang, SHEN HaiLin, WEN JingHui. Screening of Key Genes Related to Gibberellic Acid Regulation of Rachis Hardening in Honey Grapes [J]. Scientia Agricultura Sinica, 2026, 59(1): 179-189.
[3]	ZOU XiaoWei, XIA Lei, ZHU XiaoMin, SUN Hui, ZHOU Qi, QI Ji, ZHANG YaFeng, ZHENG Yan, JIANG ZhaoYuan. Analysis of Disease Resistance Induced by Ustilago maydis Strain with Overexpressed UM01240 Based on Transcriptome Sequencing [J]. Scientia Agricultura Sinica, 2025, 58(6): 1116-1130.
[4]	SUN Ping, ZHU WenCan, LIN XianRui, WU JiaQi, CAO YiWen, CHEN ChenFei, WANG Yi, ZHU JianXi, JIA HuiJuan, QIAN MinJie, SHEN JianSheng. Effects of Rainy and Low Light Conditions on Coloration and Flavonoid Accumulation in Peach Peel Based on Metabolomic and Transcriptomic Analyses [J]. Scientia Agricultura Sinica, 2025, 58(6): 1173-1194.
[5]	XIE LuLu, LI Fu, ZHANG SiYuan, GAO JianChang. Analysis of Conserved Genes in Adventitious Root Formation Based on Cross Species Transcriptomes [J]. Scientia Agricultura Sinica, 2025, 58(6): 1195-1209.
[6]	GAO YanHao, WANG TingTing, BAI WeiWei, DU XingJie, LIU Xian, QIN BenYuan, FU Tong, SUN Yu, GAO TengYun, ZHANG TianLiu. The Combination of Lipidome and Transcriptome Revealed the Differential Expression Patterns of Lipid Characteristics in Different Muscle Tissues for Nanyang Cattle [J]. Scientia Agricultura Sinica, 2025, 58(6): 1239-1258.
[7]	WANG Fan, LIU ChenWei, LU HongChen, XU RenChao, BIAN XiaoChun. Transcriptome Analysis of Vicia faba Response to Alternaria alternata Infection and Validation of the Disease Resistance Function of VfPR4 [J]. Scientia Agricultura Sinica, 2025, 58(22): 4656-4672.
[8]	MU YingTong, LU JingShi, ZHANG YuTong, SHI FengLing. Identification of Key Drought-Responsive Genes in Upright Medicago ruthenica Sojak cv. Zhilixing Based on Transcriptome Sequencing and WGCNA [J]. Scientia Agricultura Sinica, 2025, 58(21): 4528-4543.
[9]	PAN Yuan, WANG De, LIU Nan, MENG XiangLong, DAI PengBo, LI Bo, HU TongLe, WANG ShuTong, CAO KeQiang, WANG YaNan. Evaluation of the Effectiveness of Two High-Throughput Sequencing Techniques in Identifying Apple Viruses and Identification of Two Novel Viruses [J]. Scientia Agricultura Sinica, 2025, 58(2): 266-280.
[10]	LIN Yan, ZHENG LingLing, TIAN Jia, WEN Yue, CHEN Chen, WANG Lei. Based on Transcriptomics and Proteomics to Provide Insights into the Molecular Mechanisms of Calyx Abscission in Korla Xiangli [J]. Scientia Agricultura Sinica, 2025, 58(18): 3744-3765.
[11]	DONG Xue, CHEN MengQiu, SHAO Jin, WU XueYou, TANG PeiAn. Construction of a Differential Gene Expression and Quality Regulation Network in Stored Rice Grain Using WGCNA [J]. Scientia Agricultura Sinica, 2025, 58(14): 2885-2903.
[12]	CAO YanYong, CHENG ZeQiang, MA Juan, YANG WenBo, ZHU WeiHong, SUN XinYan, LI HuiMin, XIA LaiKun, DUAN CanXing. Integrating Transcriptomic and Metabolomic Analyses Reveals Maize Responses to Stalk Rot Caused by Fusarium proliferatum [J]. Scientia Agricultura Sinica, 2025, 58(1): 75-90.
[13]	LIN Wei, WU ShuiJin, LI YueSen. Transcriptome and Proteome Association Analysis to Revealthe Molecular Mechanism of Baxi Banana Seedlings in Response to Low Temperature [J]. Scientia Agricultura Sinica, 2024, 57(8): 1575-1591.
[14]	GAO ChenXi, HAO LuYang, HU Yue, LI YongXiang, ZHANG DengFeng, LI ChunHui, SONG YanChun, SHI YunSu, WANG TianYu, LI Yu, LIU XuYang. Analysis of Transposable Element Associated Epigenetic Regulation under Drought in Maize [J]. Scientia Agricultura Sinica, 2024, 57(6): 1034-1048.
[15]	HAN XuDong, YANG ChuanQi, ZHANG Qing, LI YaWei, YANG XiaXia, HE JiaTian, XUE JiQuan, ZHANG XingHua, XU ShuTu, LIU JianChao. QTL Mapping and Candidate Gene Screening for Nitrogen Use Efficiency in Maize [J]. Scientia Agricultura Sinica, 2024, 57(21): 4175-4191.