莲藕多酚氧化酶互作蛋白的筛选及验证

doi:10.3864/j.issn.0578-1752.2020.18.013

Abstract

Abstract:

【Objective】The protein interacting with NnPPO1 (GenBank: ADC92563.1), a member of the polyphenol oxidase family of China Lotus (Nelumbo nucifera Gaertn.), was screened and verified, which laid the foundation for further studying of the molecular mechanism of PPO and precise inhibition of its activity in Nelumbo nucifera. 【Method】The interaction between Nelumbo nucifera antioxidant enzyme and NnPPO1 was verified by yeast double hybridization (Y2H). The toxicity and self-activation activity of the interacting protein catalase isoenzyme NnCAT1 (GenBank: XP_010242894.1) were detected by the transformed yeast experiment. The expression vectors of 35S-NnPPO1-SPYNER173 and 35S-NnCAT1-SPYCEM used in the BiFC experiment were constructed. The recombinant plasmid was transformed into tobacco by an agrobacteria-mediated method to further verify the interaction between NnPPO1 and NnCAT1. The proteins were truncated based on the domains of NnPPO1 and NnCAT1 to find the key domains of interaction. PlantCARE and real-time fluorescent quantitative PCR (qRT-PCR) were used to analyze the promoter-related components of NnPPO1 and NnCAT1 as well as their expression levels in different tissues of Nelumbo nucifera. The 35S-NnCAT1-GFP fusion protein expression vector was constructed by homologous recombination method to analyze subcellular localization. DNAMAN software was used to perform multi-sequence alignment between the NnCAT1 sequence and other species sequences. The basic character and structure of NnCAT1 were analyzed by using bioinformatics website. 【Result】NnCAT1 interacts with NnPPO1, had no self-activation or toxicity to yeast. The interaction between NnPPO1 and NnCAT1 was further confirmed by the yellow fluorescence signal observed on the cell membrane and nucleus. The tyrosinase domain of NnPPO1 played a major role in protein interactions. NnPPO1 and NnCAT1 promoter sequences contained a variety of cis-regulatory elements, such as photoresponse element (Box 4, GT1-motif, and TCT-motif), stress response element (ARE), and hormone response element (CGTCA-motif and TGACG-motif). The expression patterns of NnPPO1 and NnCAT1 were almost the same. They were expressed in all tissues, with the highest expression in leaf and lowest expression in stem tip. Subcellular localization showed that NnCAT1 was localized in the cell membrane and nucleus. NnCAT1 had a molecular weight of 57.0 kD and a theoretical isoelectric point (PI) of 6.93. This protein was a homologous tetramer hydrophilic protein without transmembrane structure and signal peptide, and its chloroplast transport peptide was located in the former 21 aa. The secondary structure was composed of 27.64% α-helix, 15.65% extended chain, 6.30% β-turn and 50.41% random coil. 【Conclusion】Firstly, NnCAT1 interacted with NnPPO1, and the authenticity of the interaction was further verified by the BiFC test. Secondly, the conserved tyrosinase domain of NnPPO1 played a major role in the interaction. NnPPO1 and NnCAT1 had almost the same expression pattern, and they might lead to browning of fruits and vegetables such as Nelumbo nucifera through a synergistic effect.

Key words: Nelumbo nucifera, NnPPO1, NnCAT1, protein interaction, subcellular localization, tissue-specific expression

YUAN XinBo,CHENG TingTing,XI XiaoHan,CHEN ZhangYu,WANG RuiHong,KE WeiDong,GUO HongBo. Screening of Polyphenol Oxidase Interaction Proteins from Nelumbo nucifera and Their Verification[J].Scientia Agricultura Sinica, 2020, 53(18): 3777-3791.

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Figures/Tables 11

Table 1

PCR primer list"

引物名称Name	序列Sequence	用途Use
APX-EcoR I-F	ggaggccagtgaattcATGGCTTGTCTGGGCGGTGC	酵母双杂交 Yeast two hybrid
APX-Xho I-R	tcatctgcagctcgagTCATGTTGAGAAACCCTCAGGAGG
NnCAT1-EcoR I-F	ggaggccagtgaattcATGGATCCTTACAAGTATCGCCC
NnCAT1-Xho I-R	tcatctgcagctcgagTCACATGCTCGGCTTCAC
cytCuZnSOD-EcoR I-F	ggaggccagtgaattcATGGTGAAGGCTGTTGCGG
cytCuZnSOD-Xho I-R	tcatctgcagctcgagTTAACCCTGCAAGCCAATAATACC
PER1-EcoR I-F	ggaggccagtgaattcATGCCTGGACTGACGATCGG
PER1-Xho I-R	tcatctgcagctcgagTCAGACGTTGGTAAAACGGAG
POD3L-EcoR I-F	ggaggccagtgaattcATGAGGACTACTTATCTTCCGTT
POD3L-Xho I-R	tcatctgcagctcgagTTAAGGGTTCACAACCGCACACT
POD43L-EcoR I-F	ggaggccagtgaattcATGGCTCTGGTTTTTGCTC
POD43L-Xho I-R	tcatctgcagctcgagTCAATTGAAAGACCTGCAGGAC
PODP7L-EcoR I-F	ggaggccagtgaattcATGGCCTCCATCATCACCC
PODP7L-Xho I-R	tcatctgcagctcgagTCAGTTCACTCTCCTGCAATTC
NnCAT1^16-398-EcoR I-F	ggaggccagtgaattcATGTTCTGGAGCACAAATTCTGG
NnCAT1^16-398-Xho I-R	tcatctgcagctcgagTCAATCATACCTTGAAGGAAAGTAA
NnCAT1^16-493-EcoR I-F	ggaggccagtgaattcATGTTCTGGAGCACAAATTCTGG
NnCAT1^16-493-Xho I-R	tcatctgcagctcgagTCACATGCTCGGCTTCAC
NnPPO1^175-380-Nco I-F	ctgcatatggccatgATGCCACGTAACTTCACGCA
NnPPO1^175-380-BamH I-R	gcaggtcgacggatcTCATATTGTCCACATCCGGTCGAC
NnPPO1^381-468-NcoI-F	ctgcatatggccatgATGTGGAAAAAGCTGGGAGG
NnPPO1^381-468-BamH I-R	gcaggtcgacggatcTCACTCACTGACGGTTGGC
NnPPO1^469-597-Nco I-F	ctgcatatggccatgATGTTCCCCAAAGAACTTGATGCG
NnPPO1^469-597-BamH I-R	gcaggtcgacggatcTCACGAAGCGAACACTATCTTG
NnCAT1-BD-Nco I-F	ctgcatatggccatgATGGATCCTTACAAGTATCGCCC	自激活验证 Self-activated tast
NnCAT1-BD-BamH I-R	gcaggtcgacggatcTCACATGCTCGGCTTCAC	自激活验证 Self-activated tast
NnPPO1-BiFC-N-BamH I-F	cccaggcctactagtggatccATGGCGTCGCTGTCTCCC	双分子荧光标记 BiFC
NnPPO1-BiFC-N-Xho I-R	cccgggagcggtaccctcgagTCACGAAGCGAACACTATCTTGA
NnCAT1-BiFC-C-BamH I-F	tggcgcgccactagtggatccATGGATCCTTACAAGTATCGCCC
NnCAT1-BiFC-C-Xho I-R	cccgggagcggtaccctcgagCATGCTCGGCTTCACATTGA
NnCAT1-GFP-BamH I-F	ggactctagaggatccATGGATCCTTACAAGTATCGCCC	亚细胞定位 Subcellulcar locatization
NnCAT1-GFP-Kpn I-R	cccttgctcaccatggtaccCATGCTCGGCTTCACATTGAGAC	亚细胞定位 Subcellulcar locatization
β-actin-F	GCCATCCAGGCCGTTCTCTC	qRT-PCR
β-actin-R	GGGACAGTGTGGCTGACACC
NnPPO1-qPCR-F	CAAACTCCGCGATGCCAAGC
NnPPO1-qPCR-R	CGTCGAGCCATTGGACACCA
NnCAT1-qPCR-F	TTTGCCCTGGCGTTGTGGTC
NnCAT1-qPCR-R	GGTGAGCACACTTGGGAGCA

Table 1

Fig. 1

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Fig. 3

Fig. 4

Fig. 5

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Table 2

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Fig. 9

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元件名称Elements name	功能Function	所属序列Owned sequence
GT1-motif	光响应元件Light responsive element	NnPPO1、NnCAT1
ARE	厌氧诱导必不可少的顺式作用调节元件 Cis-acting regulatory element essential for the anaerobic induction	NnPPO1、NnCAT1
CGTCA-motif、TGACG-motif	MeJA反应性中涉及的顺式作用调节元件 Cis-acting regulatory element involved in the MeJA-responsiveness	NnPPO1、NnCAT1
CAAT-box	启动子和增强子区域常见的顺式作用元件 Common cis-acting element in promoter and enhancer regions	NnPPO1、NnCAT1
Box 4	涉及光响应性的保守DNA模块的一部分 Part of a conserved DNA module involved in light responsiveness	NnPPO1、NnCAT1
TCT-motif	光响应元件的一部分Part of a light responsive element	NnPPO1、NnCAT1
TATA-box	转录启动子周围-30个核心启动子 Core promoter element around -30 of transcription start	NnPPO1、NnCAT1
P-box	赤霉素反应元件Gibberellin-responsive element	NnPPO1
A-box	顺式作用调节元件Cis-acting regulatory element	NnCAT1
ABRE	脱落酸反应性涉及的顺式作用元件 Cis-acting element involved in the abscisic acid responsiveness	NnCAT1
MBS	MYB结合位点参与干旱诱导MYB binding site involved in drought-inducibility	NnCAT1
TC-rich repeats	参与防御和应激反应的顺式作用元件 Cis-acting element involved in defense and stress responsiveness	NnCAT1
TCA-element	水杨酸反应性涉及的顺式作用元件 Cis-acting element involved in salicylic acid responsiveness	NnCAT1
G-box	参与光响应的顺式作用调节元件 Cis-acting regulatory element involved in light responsiveness	NnCAT1

Screening of Polyphenol Oxidase Interaction Proteins from Nelumbo nucifera and Their Verification

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[3]	ZHANG Rui,ZHANG XueYao,ZHAO XiaoMing,MA EnBo,ZHANG JianZhen. Antibody Preparation and Subcellular Localization of LmKnk3-5′ in Locusta migratoria [J]. Scientia Agricultura Sinica, 2022, 55(2): 329-338.
[4]	XU HuanHuan,LI Yi,GAO Wei,WANG YongQin,LIU LeCheng. Cloning and Identification of γ-Glutamyl Transpeptidase AcGGT Gene from Onion (Allium cepa) [J]. Scientia Agricultura Sinica, 2021, 54(19): 4169-4178.
[5]	WANG Hao,YIN Lian,LIU JieXia,JIA LiLi,DING Xu,SHEN Di,FENG Kai,XU ZhiSheng,XIONG AiSheng. The Carotenoid Cleavage Dioxygenases Gene AgCCD4 Regulates the Pigmentation of Celery Tissues with Different Colors [J]. Scientia Agricultura Sinica, 2021, 54(15): 3279-3294.
[6]	ZHANG ZhiXing,MIN XiuMei,SONG Guo,CHEN Hua,XU HaiLong,LIN WenXiong. Identification of 14-3-3 Client Proteins in Rice Grains and Their Response to Exogenous Hormones During the Grain Filling Stage [J]. Scientia Agricultura Sinica, 2021, 54(12): 2523-2537.
[7]	SUN HongYing,WANG Yan,LI WeiJia,ZHU TianShu,JIANG Ying,XU Yan,WU QingYue,ZHANG ZhiHong. Expression Characteristics and Function of FveD27 in Woodland Strawberry [J]. Scientia Agricultura Sinica, 2021, 54(10): 2179-2191.
[8]	LIU JiaoJiao,WANG XueMin,MA Lin,CUI MiaoMiao,CAO XiaoYu,ZHAO Wei. Isolation, Identification, and Response to Abiotic Stress of MsWRKY42 Gene from Medicago sativa L. [J]. Scientia Agricultura Sinica, 2020, 53(17): 3455-3466.
[9]	GE Ting,HUANG Xue,XIE RangJin. Cloning, Subcellular Localization and Expression Analysis of CitPG34 in Citrus [J]. Scientia Agricultura Sinica, 2019, 52(19): 3404-3416.
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[11]	ZHANG HuiYuan,LIU YongWei,YANG JunFeng,ZHANG ShuangXi,YU TaiFei,CHEN Jun,CHEN Ming,ZHOU YongBin,MA YouZhi,XU ZhaoShi,FU JinDong. Identification and Analysis of Salt Tolerance of Wheat Transcription Factor TaWRKY33 Protein [J]. Scientia Agricultura Sinica, 2018, 51(24): 4591-4602.
[12]	ZHAO QingQing, LI JunPing, LIANG LiBin, HUANG ShanYu, ZHOU ChenChen, ZHAO YuHui, WANG Qian, ZHOU Yuan, JIANG Li, CHEN HuaLan, LI ChengJun. Interaction between Influenza Virus PA Protein and Host Protein PCBP1 [J]. Scientia Agricultura Sinica, 2018, 51(17): 3389-3396.
[13]	WEI ZhouLing, PENG HaoRan, PAN Qi, ZHANG YongZhi, PU YunDan, WU GenTu, QING Ling, SUN XianChao. Subcellular Localization of the Ribosome-Inactivating Protein α-MC and Its Antiviral Effect on TMV [J]. Scientia Agricultura Sinica, 2017, 50(5): 840-848.
[14]	LIU Wei, LIU Hao, DONG ShuangYu, GU FengWei, CHEN ZhiQiang, WANG JiaFeng, WANG Hui. Construction of Rice Leaf Sheath Protoplast Transformation System and Transient Expression of Pik-H4 and AvrPik-H4 Proteins [J]. Scientia Agricultura Sinica, 2017, 50(23): 4575-4584.
[15]	PENG HaoRan, PU YunDan, ZHANG YongZhi, XUE Yang, WU GaiXia, QING Ling, SUN XianChao. Subcellular Localization and Expression Analyses of IP-L Protein Interacting with ToMV Coat Protein [J]. Scientia Agricultura Sinica, 2017, 50(17): 3344-3351.