光谱对水稻灌浆期剑叶光合及叶绿素荧光特性的影响

doi:10.3864/j.issn.0578-1752.2019.16.003

Abstract

Abstract:

【Objectives】 The study was aimed to explore the effects of different spectra on the photosynthetic and chlorophyll fluorescence characteristics of flag leaves in rice at grain filling stage. 【Method】 The rice (Oryza sativa L. cv. Huageng 5) was planted under four different light conditions, including red light of 660 nm (R660) and 630 nm (R630), blue light of 460 nm (B460) and 440 nm (B440) combined with a special plant lamp (W), and the photosynthetic and chlorophyll fluorescence characteristics of rice leaves were determined at grain filling stage. 【Result】 Compared with red light (R660+W and R630+W), blue light (B460+W and B440+W) could increase the maximum photochemistry efficiency (F_v/F_m), actual photochemical efficiency (ΦPSⅡ), electron transfer efficiency (ETR), non-photochemistry quenching (NPQ) and photochemical quenching (qP). The net photosynthetic rate in the late stage of grain filling under B460+W was higher than that under red light. Compared to those of other treatments, the panicles per unit area under R660+W were the greatest, but the 1000-grain weight and seed setting rate were both higher under B460+W. In addition, B460+W also increased the maximum net photosynthetic rate (Pn_max), initial slope of light response curve (α), quantum efficiency of dark respiration, compensation point quantum efficiency, eigen-absorption cross-section of photosynthetic pigment molecule (σ_ik) and number of light-harvesting pigment molecule (N_o). 【Conclusion】 The stable in photosynthetic rate of flag leaves under the addition of 460 nm blue light conditions was mainly attributed to increase ФPSII, contributing to accumulate dry matter, finally improved the 1000-grain weight and seed setting rate of rice.

Key words: spectral distribution, fitting of light response curve, rice 1000-grain weight, fluorescence parameter, photosystems II

LI JiangPeng,LIU HaiJun,HUANG ZhiWu,LIU XiaoYing,YOU Jie,XU ZhiGang. Effects of Spectral Distribution on Photosynthetic and Chlorophyll Fluorescence Characteristics of Flag Leaves at Grain Filling Stage in Rice[J].Scientia Agricultura Sinica, 2019, 52(16): 2768-2775.

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References 34

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处理 Treatment	穗数 Spike number (m^-2)	穗粒数 Grain number per spike	千粒重 1000-grain weight (g)	结实率 Seed setting rate (%)	产量 Yield (kg·m^-2)
R660	129.38±9.31a	42.6±2.58a	28.6±0.10a	90.23±0.22c	0.142
R630	106.99±4.98b	36.8±3.02a	28.0±0.20b	93.68±0.29a	0.103
B460	104.50±3.05b	41.3±2.58a	28.9±0.12a	94.57±0.33a	0.118
B440	114.45±7.25ab	41.8±2.94a	27.9±0.12b	92.79±0.34b	0.124

光合参数 Photosynthesis parameter	处理 Treatment
光合参数 Photosynthesis parameter	R660	R630	B460	B440
光响应曲线初始斜率 Initial slope of light response curve, α, mol·mol^-1 (×10^-2)	4.73	4.14	5.81	3.03
光抑制项 Photo-inhibition term, β, (m²·s)/天线色素分子^-1 (×10^-5)	5.75	5.23	6.65	13.5
光饱和项 Photo-saturation term, γ, (m²·s)/天线色素分子^-1 (×10^-3)	1.90	1.70	1.50	0.90
暗呼吸的量子效率 Quantum efficiency of dark respiration (×10^-2)	5.03	4.44	6.16	3.22
补偿点的量子效率 Compensation point quantum efficiency (×10^-2)	4.45	3.86	5.48	2.85
捕光色素分子的本征光能吸收截面 Eigen-absorption cross-section of photosynthetic pigment molecule, σ_ik, m² (×10^-20)	4.25	3.49	4.86	3.46
捕光色素分子数 Number of light-harvesting pigment molecule (N_o) (×10¹⁴)	5.89	6.28	6.33	4.63

指标Index	R660	R630	B460	B440
最大光化学效率F_v/F_m	0.811±0.002b	0.814±0.001ab	0.819±0.002a	0.818±0.002aa
光下最大光化学效率F_v'/F_m'	0.591±0.025aa	0.608±0.018a	0.555±0.005a	0.591±0.010a
实际光化学效率ΦPSⅡⅡ	0.436±0.023ab	0.406±0.010b	0.434±0.003ab	0.469±0.009a
电子传递速率ETR	124.17±6.66abab	115.18±2.95b	123.41±0.89ab	133.17±2.72a
非光化学淬灭NPQ	0.805±0.117bb	1.030±0.150ab	1.238±0.056a	1.008±0.049ab
1-qP	0.263±0.014bb	0.328±0.031a	0.217±0.008bc	0.208±0.006c
光化学淬灭系数qP	0.737±0.014bb	0.672±0.031c	0.783±0.008abb	0.792±0.006a

Effects of Spectral Distribution on Photosynthetic and Chlorophyll Fluorescence Characteristics of Flag Leaves at Grain Filling Stage in Rice

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