Scientia Agricultura Sinica ›› 2017, Vol. 50 ›› Issue (24): 4705-4713.doi: 10.3864/j.issn.0578-1752.2017.24.004

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY • Previous Articles     Next Articles

Re-Evaluation of Using the Color Difference Between the Top 3rd Leaf and the 4th Leaf as a Unified Indicator for High-Yielding Rice

LING QiHong, WANG ShaoHua, DING YanFeng, LI GangHua   

  1. Nanjing Agricultural University/Jiangsu Collaborative Innovation Center for Modern Crop Production/National Engineering and Technology Center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China, Nanjing 210095
  • Received:2017-02-15 Online:2017-12-16 Published:2017-12-16

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Abstract: 【Objective】 To re-evaluate if the value of color different between the top 3rd leaf and the 4th leaf would be a unified indicator for high-yielding rice.【Method】Two experiments were carried out with different leaf color cultivars, including four japonica rice cultivars (Jinnanfeng, 9915, Yueguang, 9325) and two indica rice cultivars (Baidao, H97-32) in 2001 and 2002. One experiment applied different base nitrogen fertilizer as 0, 6.5, 13.0, 19.5 and 26.0 g·m-2. Another experiment applied different panicle nitrogen fertilizer as 0, 6.5, 13.0, 19.5 and 26.0 g·m-2, followed by base nitrogen as 13.0 g·m-2. SPAD values of top 4 leaves were measured using the Chlorophyll meter SPAD-502 at N-n stage, the 2nd leaf-age from top and full heading stage. The relationship of the different SPAD value of the top 4th leaf to the top 3rd leaf with plant nitrogen content and yield was investigated. 【Result】 It is difficult to diagnose rice nitrogen status base on single leaf’s SPAD value, because the value is changing if cultivar or rice growth period changed. The three kinds of leaf color differences of top in each growth stage showed: top 4 top 3, which were the exact physiological reflection of nitrogen deficiency, normal and excess. There is no regular relationship of the SPAD of single leaf with tillering rate, the percent of tillers to panicles, spikelet number per panicle and setting rate, so the SPAD of single leaf can’t be used as a common diagnostic indicator. However, there is a regular relationship between different value form the top 3rd leaf to the 4th leaf and yield components. Furthermore, rice should have highest yield if the different value is zero at N-n stage, the 2nd leaf-age from top and full heading stage. 【Conclusion】 The difference color between the top 3rd leaf and the 4th leaf is the internal indicator of nitrogen nutrition level in rice. It can diagnosis rice nitrogen nutrition situation well and truly with comparing color of the top 3rd and 4th leaf, coordinating diagnosis single leaf’s color.

Key words: rice, leaf color difference, diagnosis, high yield, nitrogen nutrient

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