大麦近等基因系苗期根系形态及叶片渗透调节物质对PEG胁迫的响应

doi:10.3864/j.issn.0578-1752.2017.13.002

Abstract

Abstract: 【Objective】The aim of this study was to assess the relationship between the semi-dwarf gene uzu of barley and the drought resistance and to provide a theoretical basis for drought resistance and high quality breeding of barley.【Method】A hydroponic experiment was carried out to study the plant height, root-shoot ratio, root morphology and leaf osmoregulation substances of seedling of two pairs barley genotypes with different heights under different drought stress levels resulted by PEG6000 at different concentrations (0, 5%, 15%, 25%). Samples were taken during seedlings grew to four leaves and a heart. Plant height was measured by ruler. Root-shoot ratio was calculated by root biomass to aboveground biomass ratio multiplied by 100%, which were obtained by drying and weighing. Total root length, root surface area and volume were measured and analyzed by root system scanner and the WinRHIZO software. The soluble sugar, soluble protein and proline content were determined by sulfuric acid-anthrone colorimetry, coomassie brilliant blue G-250 dyeing method and acidic indene three ketone chromogenic method, respectively.【Result】Analysis showed that barley seedling growth was significantly inhibited, and the plant height decreased gradually, while the opposite trend showed in the root-shoot ratio with the increase of stress concentration. In terms of root morphology, as the stress concentration increased, two pairs near-isogenic line showed different trends. The change range of 3D and 15T was less than that of 3T and 15D, respectively, under the mild and moderates tress, while the change range of 3D and 15T showed an opposite trend under severe stress. Furthermore, soluble sugar content showed an increase at first and then a decrease and an increase again with the increase of the stress concentration, while the opposite trend showed in soluble protein content. The change range was characterized by 3D < 3T, 15D < 15T under the mild and moderate stress, and the change range of 3D and 15D also showed an opposite trend under severe stress. But the proline content presented a tendency of increasing with the increase of the stress concentration, and the small variations were characterized in 3D and 15D under different treatments. Correlation analysis indicated that plant height showed significant correlation with other traits in addition to the soluble protein content. There was also a significant relationship between root-shoot ratio and root morphology under different treatments. Meanwhile, the strains with higher soluble sugar content also had higher proline content, while the soluble protein content was low. Moreover, root morphology also had certain correlation with leaf osmoregulation substances, namely, the total root length and root surface area had significant negative relationship with soluble sugar content and proline content, respectively.【Conclusion】In the matter of root morphology and leaf osmoregulation, there are different responses under drought stress and influence with each other at seedling stage of barley genotypes with different heights. Under the mild and moderate stress, the semi-dwarf gene in the near-isogenic lines 3T and 3D can resist drought stress.

Key words: barley, near-isogenic line of plant height, PEG stress, root morphology, osmoregulation

DU Huan, MA TongTong, GUO Shuai, ZHANG Ying, BAI ZhiYing, LI CunDong. Response of Root Morphology and Leaf Osmoregulation Substances of Seedling in Barley Genotypes with Different Heights to PEG Stress[J].Scientia Agricultura Sinica, 2017, 50(13): 2423-2432.

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