灰飞虱鞘氨醇激酶时空表达分析及其对杀虫剂胁迫的响应

doi:10.3864/j.issn.0578-1752.2016.22.011

Abstract

Abstract: 【Objective】The objective of this study is to understand the characteristics of sphingosine kinase (SK) and research on the spatio-temporal expression of SK and its response to insecticide stress in Rice stripe virus (RSV) -infected Laodelphax striatellus Fallén (small brown planthopper, SBPH) and RSV-free L. striatellus.【Method】A SK sequence (LsSK) from L. striatellus was cloned using PCR. qRT-PCR was employed to analyze the expression pattern of SK gene at different stages (1st-5th instar nymphs, female and male adult) and various organs (head, salivary, midgut, malpighian tubules, ovary and spermary) of the two above mentioned L. striatellus colonies. In order to calculate the sublethal concentration (LC₅₀) of three insecticides (deltamethrin, buprofezin and imidacloprid), those insecticides solutions were applied topically to the mesonotum of L. striatellus 4th-instar nymphs with a hand microapplicator. The SK mRNA level of L. striatellus was detected after exposure to LC₅₀ of three insecticides. After knockdown SK of two L. striatellus colonies by using dsRNA injection, L. striatellus was exposed to LC₅₀ of three insecticides and the mortality was recorded.【Result】a LsSK sequence from L. striatellus was cloned and its length is 1 282 bp(GenBank accession number KT989975). According to the phylogenetic analysis, SK of L. striatellus was clustered together with those from hemipteran insects. The amino-acid sequences of LsSK contained four conserved domains (C1-C4), and the catalytic domain–Diacylglycerol active site formed within C1-C3. qRT-PCR results showed that LsSK was most highly expressed in the 4th-instar nymphs of RSV-infected L. striatellus, followed by 3rd-instar nymphs. In RSV-free L. striatellus, LsSK was most highly expressed in 5th-instar nymphs, followed by 1st- and 4th-instar nymphs. Furthermore, LsSK levels were significantly higher in RSV-infected 3rd- and 4th-instar nymphs than that in RSV-free 3rd- and 4th-instar nymphs(P＜0.05). At the adult stage, LsSK levels were significantly higher in RSV-infected adults than in RSV-free adults (P＜0.05). Interestingly, LsSK showed extremely higher expression in the tissues of viruliferous males than that of viruliferous females and nonviruliferous adults. The highest expression of LsSK was found in salivary and malpighian tubules of viruliferous males, followed by heads. After exposure to sublethal concentrations (LC₅₀) of three insecticides (imidacloprid 6.5 ng·μL^-1, buprofezin 500 ng·μL^-1 and deltamethrin 37.5 ng·μL^-1), the mRNA levels of LsSK and response speed of two L. striatellus colonies were different. Exposure to buprofezin led to significantly increased expression of LsSK while no significant changes was observed after exposure to deltamethrin(P＞0.05). The response of LsSK expression to buprofezin was relatively faster than to the other insecticides. Injection of L. striatellus with dsRNA against SK reduced the mRNA level of LsSK greatly, and silencing of SK led to increased susceptibility in RSV-infected and RSV-free L. striatellus to these three insecticides.【Conclusion】The LsSK was identified and cloned, and it was found significantly high expression in the 3rd and 4th instar of RSV-infected L. striatellus. Silencing the SK resulted in increased susceptibility in L. striatellus to three insecticides, indicated that SK plays a role in facilitating L. striatellus to response to insecticidal stress.

Key words: sphingolipid, sphingosine kinase, Laodelphax striatellus, RNA interference, qRT-PCR

JIAO Wen-juan, LI Fei-qiang, ZHANG Min-jing, SHI Xiao-xiao, ZHU Mu-fei, MAO Cun-gui, ZHU Zeng-rong. Spatio-Temporal Expression of Sphingosine Kinase and Its Response to Insecticide Stress in Laodelphax striatellus Fallén[J].Scientia Agricultura Sinica, 2016, 49(22): 4387-4397.

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Spatio-Temporal Expression of Sphingosine Kinase and Its Response to Insecticide Stress in Laodelphax striatellus Fallén

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