变温条件下钙对山定子根系线粒体功能的影响

doi:10.3864/j.issn.0578-1752.2014.19.015

Abstract

Abstract: 【Objective】 The responding mechanisms of mitochondrial function in Malus baccata Borkh. roots and the regulating role of calcium under changing temperature stress were studied, and the results would be a theoretical foundation for further study of the physiological mechanism of M. baccata Borkh. roots under such kind of temperature stress. 【Method】 The seedlings of M. baccata Borkh. were used as materials and treated with or without 2% CaCl₂ solution, to investigate the characteristics of mitochondrial function, such as mitochondrial membrane permeability transition (MPT), mitochondrial membrane potential (?ψm), super oxygen anion () generation rate, electron transfer pathway activity and contribution rate (cytochrome pathway, CP; alternative pathway, AP), CaM content, and CaN content under violently changing temperature (5℃→20℃→0℃). 【Result】 In control, MPT, generation rate and MDA content in M. baccata Borkh. roots obviously increased under violently changing temperature, ?ψm decreased at the same time. Compared with the control, CaCl₂ treatment maintained the MPT at a relatively stable level during temperature changing. At the cooling stage, ?ψm significantly rose in CaCl₂ treatment, meanwhile ,the generation rate of gradually dropped.CaCl₂ treatment kept MDA content at a relatively lower level. The activity and contribution rate of CP in control descended as temperature going up, and thenincreased with temperature going down, while AP activity kept in ascending under temperature treatment. Comparing with the control, CaCl₂ remarkably increased the activities of CP and AP at 20℃. Moreover, the varying trends of AP activity and contribution rate were opposite to that of CP. In control, CaM content consistently increased, while CaN content decreased firstly and then increased. The content of CaM in CaCl₂ treatment declined with temperature going up, and then increased as temperature going down, while CaN content steadily decreased.【Conclusion】Violently changing temperature inhibited the function of mitochondria in roots and increased electronic leak, finally exacerbated the membrane lipid peroxidation. The extent of membrane lipid peroxidation could be effectively alleviated by CaCl₂ treating through stabilizing MPT, adjusting CP and AP activities. At warming stage, CaCl₂ treatment alleviated temperature stress by the signal mode of decreasing CaM content and increasing CaN content, and at cooling stage, by the signal mode of increasing CaM and decreasing CaN content.

Key words: Malus baccata Borkh., temperature stress, mitochondria, calcium signaling

SU Hong, LI Li-jie, MA Huai-yu, Lü De-guo, SUN Jing. Effects of Calcium on Mitochondrial Function in Malus baccata Borkh. Roots Under Changing Temperature[J].Scientia Agricultura Sinica, 2014, 47(19): 3866-3873.

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Effects of Calcium on Mitochondrial Function in Malus baccata Borkh. Roots Under Changing Temperature

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