荧光假单胞菌对樱桃根系呼吸和幼苗生长的影响

doi:10.3864/j.issn.0578-1752.2014.19.014

Abstract

Abstract: 【Objective】 Rational utilization of soil microbial for improving soil environment is helpful to promote plant yield and quality. The adaptation of cherry root is bad, so it is sensitive to the change of soil environment. The objective of the experiment is to discuss the mechanisms of Cerasus sachalinensis (Fr.Schu) seedlings responding to soil sterilization and inoculation of Pseudomonas fluorescens, thus providing an academic and practical basis for regulating the rhizosphere soil.【Method】In order to find the influence of inoculation P. fluorescens on plant root function, C. sachalinensis(Fr.Schu) seedlings were used as materials to measure the correlative indexes of root respiration metabolism.【Result】Compared with soil sterilization, glycolysis, tricarboxylic acid cycle, and cytochrome oxidase pathways were also the dominant respiratory pathways from 5 d to 50 d after C. sachalanensis seedlings were subjected to CK and SS+Pseu treatments. On 5th d, there was no significant difference in total respiration rate of the roots between SS+Pseu treatment and CK, however, on 20 and 35th d, in SS+Pseu treatment, the total respiration rate of the roots was lower than that in the CK, while the total respiration rate of the roots in the SS+Pseu treatment was higher than that in CK on 50th d. On 5th d, the activities of PK, MDH, G-6-PDH, SDH were significantly increased in the SS+Pseu treatment, however, the activity of PFK was significantly decreased. On 20th d, the activity of PK was significantly increased in the SS+Pseu treatment, however, the activities of PFK and G-6-PDH were significantly decreased. On 35th d the, activity of PFK was increased by 127.0%, On 50th d the activity of G-6-PDH was increased by two times. Though, there were some differences between CK and SS+Pseu treatment, however, these differences were not significant. In SS+Pseu treatment the stem diameter and leaves number were significantly increased by 9.3% and 16.7%. Inoculation with P. fluorescens was helpful to plant growth.【Conclusion】Inoculation with P. fluorescens could influence plant growth and root function. In general, 20 d after the end of continuous inoculation (six times) with the bacterial liquid whose OD value was 0.4 could change the rate of respiration pathways, key enzyme activities via influence the function of roots, and then promoted plant growth.

Key words: cherry; root, seedling, Pseudomonas fluorescens, respiration pathway, respiratory key enzyme

ZHANG Peng, QIN Si-jun, ZHOU Wen-jie, Lü De-guo, MA Huai-yu.

Effects of Pseudomonas fluorescens Inoculation on Root Respiration and Seedling Growth of Cherry

[J].Scientia Agricultura Sinica, 2014, 47(19): 3857-3865.

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References

[1] Hirrel M C, Gerdemann J W. Improved growth of onion and dell pepper in saline soils by two vesicular arbuscular mycorrhizal fungi. Soil Science Society of America Journal, 1980, 104(44): 654- 655.

[2] 刘灵芝, 秦嗣军, 吕德国, 王冰营. 樱桃园土壤优势放线菌的分离及其促长功能研究. 中国农业科学, 2013, 46(6): 1221-1229.

Liu L Z, Qin S J, Lü D G, Wang B Y. Isolation of dominant actinomycetes from cherry orchard soil and their functions in bio-control and plant promotion. Scientia Agriculture Sinica, 2013, 46(6): 1221-1229. (in Chinese)

[3] 吕德国, 于翠, 秦嗣军, 刘国成, 杜国栋. 本溪山樱根部解磷细菌的定殖规律及其对植株生长发育的影响. 中国农业科学, 2008, 41(2): 508-515.

Lü D G, Yu C, Qin S J, Liu G C, Du G D. Colonization regulation pattern of phosphobacteria and its effect on the growth and development of Cerasus sachalinensis. Scientia Agriculture Sinica, 2008, 41(2): 508-515. (in Chinese)

[4] Khare E, Arora N K. Effect of indole-3-acetic acid (IAA) produced by Pseudomonas aeruginosa in suppression of charcoal rot disease of chickpea. Current Microbiology, 2010, 61(1): 64-68.

[5] Sandheep A R, Asok A K, Jisha M S. Combined inoculation of Pseudomonas fluorescens and Trichoderma harzianum for enhancing plant growth of vanilla (Vanilla planifolia). Pakistan Journal of Biological Science, 2013, 16(12): 580-584.

[6] 刘健, 李俊, 姜昕, 徐玲玫, 樊蕙, 葛诚. 巨大芽胞杆菌luxAB标记菌株的根际定殖研究. 微生物学通报, 2001, 28(6): 1-4.

Liu J, Li J, Jiang X, Xu L M, Fan H, Ge C. Study on root colonization of wheat by luxAB gene-marked Bacillus megaterium ATCC14581. Microbiology, 2001, 28(6): 1-4. (in Chinese)

[7] 年洪娟, 张杰, 樊利强, 刘朔, 宋福平, 黄大昉. ARDRA 方法在荧光假单胞菌分离鉴定中的应用. 中国农业科学, 2007, 40(1): 92-98.

Nian H J, Zhang J, Fan L Q, Liu S, Song F P, Huang D F. Application of ARDRA technology in pseudomonas fluorescens isolation and identification. Scientia Agriculture Sinica, 2007, 40(1): 92-98. (in Chinese)

[8] Leeman M, Van Pelt J A, Den Ouden F M, Heinsbroek M, Bakker P A H M, Schippers B. Induction of systemic resistance by Pseudomonas fluorescens in radish cultivars differing in susceptibility to fusarium wilt, using a novel bioassay. European Journal of Plant Pathology, 1995, 101(6): 655-664.

[9] Kochar M, Upadhyay A, Srivastava S. Indole-3-acetic acid biosynthesis in the biocontrol strain Pseudomonas fluorescens Psd and plant growth regulation by hormone overexpression. Research in Microbiology, 2011, 162(4): 426-435.

[10] Meng S D, Guan G L, Wang W P, Chen L, Yang Y S. Effect of Phytohormone Produced by the rhizosphere microorganisms on wheat growth. Plant Physiology Communications, 1998, 34(6): 427-429.

[11] Smyth E M, McCarthy J, Nevin R, Khan M R, Dow J M, O’Gara F, Doohan F M. In vitro analyses are not reliable predictors of the plant growth promotion capability of bacteria; a Pseudomonas fluorescens strain that promotes the growth and yield of wheat. Journal of Applied Microbiology, 2011, 111(3): 683-692.

[12] Bouma T J, Bryla D R. On the assessment of root and soil respiration for soils of different textures: interactions with soil moisture contents and soil CO₂ concentrations. Plant and Soil, 2000, 227(1): 215-221.

[13] 秦嗣军, 吕德国, 李志霞, 马怀宇, 刘灵芝. 水分胁迫对东北山樱幼苗呼吸等生理代谢的影响. 中国农业科学, 2011, 44(1): 201-209.

Qin S J, Lü D G, Li Z X, Ma H Y, Liu L Z. Effects of water stress on respiration and other physiological metabolisms of Cerasus sachalinensis Kom. seedlings. Scientia Agriculture Sinica, 2011, 44(1): 201-209. (in Chinese)

[14] 盛浩, 杨玉盛, 陈光水, 高人, 曾宏达, 钟羡芳. 植物根呼吸对升温的响应. 生态学报, 2007, 27(4):1597-1605..

Sheng H, Yang Y S, Chen G S, Gao R, Zeng H D, Zhong X F. The dynamic response of plant root respiration to increase temperature and global warming. Acta Ecologica Sinica, 2007, 27(4): 1597-1605. (in Chinese)

[15] 寇太记, 朱建国, 谢祖彬, 刘钢, 曾青. CO₂浓度增加和不同氮肥水平对冬小麦根系呼吸及生物量的影响. 植物生态学报, 2008, 32(4): 922-931.

Kou T J, Zhu J G, Xie Z B, Liu G, Zeng Q. Effect of elevated atmospheric CO₂ concentration and level of nitrogen fertilizer on root respiration and biomass of winter wheat. Journal of Plant Ecology, 2008, 32(4): 922-931. (in Chinese)

[16] Phillips D A, Joseph C M, Yang G P, Martínez-Romero E. Identification of lumichrome as a Sinorhizobium enhancer of alfalfa root respiration and shoot growth. Proceedings of the National Academy of Sciences of the USA, 1999, 96(22): 12275-12280.

[17] Bouma T J, Yanai R D, Elkin A D, Hartmond U, Flores-Alva D E, Eissenstat D M. Estimating age dependent costs and benefits of roots with contrasting life span: comparing apples and oranges. New Phytologist, 2001, 150(3): 685-695.

[18] 毛志泉, 王丽琴, 沈向, 束怀瑞, 邹岩梅. 有机物料对平邑甜茶实生苗根系呼吸强度的影响. 植物营养与肥料学报, 2004, 10(2): 171-175.

Mao Z Q, Wang L Q, Shen X, Shu H R, Zou Y M. Effect of organic materials on respiration intensity of annual Malus hupehensis Rehd. root system. Plant Nutrition and Fertilizing Science, 2004, 10(2): 171-175. (in Chinese)

[19] 余让才, 潘瑞炽. 蓝光对水稻幼苗呼吸代谢的影响. 中国水稻科学, 1996, 10(3): 159-163.

Yu R C, Pan R C. Effect of blue light on the respiration of rice (Oryza sativa) seedlings. Chinese Journal of Rice Science, 1996, 10(3): 159-163. (in Chinese)

[20] Ling K H, Paetkau V, Marcus F, Lardy H A. Phosphofructokinase from skeletal muscles// Wood W A. ed. Methods in Enzymology. Academic press, New York, 1996: 425-426.

[21] Sridhar J, Eiteman M, Wiegel J W. Elucidation of enzymes in fermentation pathways used by Clostridium thermosuccino genes growing on inulin. Journal of Environment Microbiology, 2000, 66(1): 246-251.

[22] 欧阳光察. 植物生理学实验手册. 上海:上海科技出版社, 1985: 195-196.

Ouyang G C. Plant Physiology Lab Manual. Shanghai: Shanghai Science and Technology Press, 1985: 195-196. (in Chinese)

[23] Plaut G W E. Isocitrate dehydrogenase. Methods in Enzymology, 1969, 13: 34-41.

[24] Lamed R, Zeikus J G. Glucose fermentation pathway of Thermonaerobium brockii. Journal of Bacteriology, 1980, 141(3): 1251-1257.

[25] Dobbelaere S, Okon Y. The plant growth promoting effects and plant responses//Nitrogen Fixation: Origins, Applications and Research Progress. Springer Netherlands, 2007, 5: 145-170.

[26] Saravanakumar D, Lavanya N, Muthumeena B, Raguchander T, Suresh S, Samiyappan R. Pseudomonas fluorescens enhances resistance and natural enemy population in rice plants against leaffolder pest. Journal of Applied Entomology, 2008, 132(6): 469-479.

[27] Çakmakç? R, Erat M, Erdo?an Ü, Dönmez M F. The influence of plant growth–promoting rhizobacteria on growth and enzyme activities in wheat and spinach plants. Journal of Plant Nutrition and Soil Science, 2007, 170(2): 288-295.

[28] Cosme M, Wurst S. Interactions between arbuscular mycorrhizal fungi, rhizobacteria, soil phosphorus and plant cytokinin deficiency change the root morphology, yield and quality of tobacco. Soil Biology and Biochemistry, 2013, 57: 436-443.

[29] Jeon J S, Lee S S, Kim H Y, Ahn S T, Song G H. Plant growth promotion in soil by some inoculated microorganisms. Journal of Microbiology, 2003, 41(4): 271-276.

[30] Dey R, Pal K K, Bhatt D M, Chauhan S M. Growth promotion and yield enhancement of peanut(Arachis hypogaea L.) by application of plant growth promoting rhizobacteria. Microbiological Research, 2004, 159(4): 371-394.

[31] García-Seco D, Bonilla A, Algar E, García-Villaraco A, Mañero G J, Ramos-Solano B. Enhanced blackberry production using Pseudomonas fluorescens as elicitor. Agronomy for Sustainable Development, 2013, 33(2): 385-392.

[32] Gholami A, Shahsavani S, Nezarat S. The effect of plant growth promoting rhizobacteria (PGPR) on germination, seedling growth and yield of maize. Proceedings of World Academy of Science: Engineering & Technology, 2009, 3(1): 19-24.

[33] Wang X Z, Curtis P. A meta-analytical test of elevated CO₂ effects on plant respiration. Plant Ecology, 2002, 161(2): 251-261.

[34] Iswandi A, Bossier P, Vandenabeele J, Verstraete W. Relation between soil microbial activity and the effect of seed inoculation with the rhizopseudomonad strain 7NSK2 on plant growth. Biology and Fertility of Soils, 1987, 3(3): 147-151.

[35] 王素素, 杜国栋, 吕德国. 土壤高温处理对连作草莓根系呼吸代谢及植株发育的影响. 果树学报, 2011, 28(2): 234-239.

Wang S S, Du G D, Lü D G. Effects of soil sterilization treatments on root respiratory metabolism and development of strawberry under the replanting conditions. Journal of Fruit Science, 2011, 28(2): 234-239. (in Chinese)

[36] Gaggìa F, Baffoni L, Gioia D D. Inoculation with microorganisms of Lolium perenne L.: evaluation of plant growth parameters and endophytic colonization of roots. New Biotechnology, 2013, 30(6): 695-704.

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