Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (19): 3857-3865.doi: 10.3864/j.issn.0578-1752.2014.19.014

• HORTICULTURE • Previous Articles     Next Articles

Effects of Pseudomonas fluorescens Inoculation on Root Respiration and Seedling Growth of Cherry

ZHANG Peng, QIN Si-jun, ZHOU Wen-jie, LÜ De-guo, MA Huai-yu   

  1. College of Horticulture, Shenyang Agricultural University /Key Laboratory for Northern Fruit Tree Cultivation and Physio-Ecology of Shenyang City, Shenyang 110866
  • Received:2014-03-31 Revised:2014-06-20 Online:2014-10-01 Published:2014-10-01

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Abstract: 【Objective】 Rational utilization of soil microbial for improving soil environment is helpful to promote plant yield and quality. The adaptation of cherry root is bad, so it is sensitive to the change of soil environment. The objective of the experiment is to discuss the mechanisms of Cerasus sachalinensis (Fr.Schu) seedlings responding to soil sterilization and inoculation of Pseudomonas fluorescens, thus providing an academic and practical basis for regulating the rhizosphere soil.【Method】In order to find the influence of inoculation P. fluorescens on plant root function, C. sachalinensis(Fr.Schu) seedlings were used as materials to measure the correlative indexes of root respiration metabolism.【Result】Compared with soil sterilization, glycolysis, tricarboxylic acid cycle, and cytochrome oxidase pathways were also the dominant respiratory pathways from 5 d to 50 d after C. sachalanensis seedlings were subjected to CK and SS+Pseu treatments. On 5th d, there was no significant difference in total respiration rate of the roots between SS+Pseu treatment and CK, however, on 20 and 35th d, in SS+Pseu treatment, the total respiration rate of the roots was lower than that in the CK, while the total respiration rate of the roots in the SS+Pseu treatment was higher than that in CK on 50th d. On 5th d, the activities of PK, MDH, G-6-PDH, SDH were significantly increased in the SS+Pseu treatment, however, the activity of PFK was significantly decreased. On 20th d, the activity of PK was significantly increased in the SS+Pseu treatment, however, the activities of PFK and G-6-PDH were significantly decreased. On 35th d the, activity of PFK was increased by 127.0%, On 50th d the activity of G-6-PDH was increased by two times. Though, there were some differences between CK and SS+Pseu treatment, however, these differences were not significant. In SS+Pseu treatment the stem diameter and leaves number were significantly increased by 9.3% and 16.7%. Inoculation with P. fluorescens was helpful to plant growth.【Conclusion】Inoculation with P. fluorescens could influence plant growth and root function. In general, 20 d after the end of continuous inoculation (six times) with the bacterial liquid whose OD value was 0.4 could change the rate of respiration pathways, key enzyme activities via influence the function of roots, and then promoted plant growth.

Key words: cherry; root, seedling, Pseudomonas fluorescens, respiration pathway, respiratory key enzyme

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