Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (17): 3492-3500.doi: 10.3864/j.issn.0578-1752.2014.17.017

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES • Previous Articles     Next Articles

Construction of Genetic Map of Foxtail Millet (Setaria italica (L.) Beauv.) Using PCR-Based Molecular Markers

WANG Zhi-lan, WANG Jun, YUAN Feng, DU Xiao-fen, YANG Hui-qing, GUO Er-hu   

  1. Institute of Millet, Shanxi Academy of Agricultural Sciences, Changzhi 046011, Shanxi
  • Received:2014-02-19 Online:2014-09-01 Published:2014-06-04

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Abstract: 【Objective】 This study aims to construct a genetic map of foxtail millet (Setaria italica (L.) Beauv.) using PCR- based molecular markers. 【Method】 With 81 SSR markers distributed in nine chromosome of foxtail millet as reference markers, a total of 1 733 markers including SSR, STS, SNP, and SV from foxtail millet and ACGM and SSR from pearl millet, tall fescue and rice were used to screen polymorphic markers between Gao 146A and K103, and the polymorphic markers were tested in the F2 segregation populations derived from cross Gao 146A / K103. MAPMAKER VERSION 3.0 and MapDraw V2 were used for linkage analysis and integrated genetic map drawing.【Result】A genetic linkage map of foxtail millet containing 192 different kinds of markers was constructed, and 33 markers were newly mapped, of which 32 were from foxtail millet and 1 from pearl millet. The map consisted of nine linkage groups and covered the genome length 2 082.5 cM. Each linkage group was between 119.5-475.2 cM and the average distance of each linkage group spanned 231.39 cM. The number of markers on each linkage group ranged from 10 to 37 and the average distance between different markers was 10.85 cM. There were 36 markers accounted for 18.75% of 192 ones distributed in different linkage group and appeared segregation disorder. There appeared segregation disorder hotspots on LG2, LG6 and LG7, including 10, 15 and 7 segregation markers, respectively, and segregation disorder scattered on LG1, LG4, LG5 and LG8, however, no segregation disorder occurred on LG3 and LG9. According to transferability of molecular markers from different crops, there were 205 polymorphic markers of 1 235 ones from foxtail millet between parents and F2 population and the polymorphism rate was 16.60%, however there was only one polymorphic marker of 498 ones from pearl millet, tall fescue and rice.【Conclusion】A genetic linkage map of foxtail millet, covering the genome length 2 082.5 cM, was constructed using molecular markers from different species and most of the markers in this map were from foxtail millet.

Key words: foxtail millet , molecular marker , polymorphism , genetic map , transfer ability

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