Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (15): 3069-3076.doi: 10.3864/j.issn.0578-1752.2014.15.016

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Study on Differential Expression of Ovine RARG in Ovary at Different Estrous Cycles

 WANG  Wei-Min, HU  Ting-Xi, LI  Fa-Di, MA  You-Ji, FAN  Hong-Ying, PAN  Xiang-Yu   

  1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070
  • Received:2013-07-08 Online:2014-08-01 Published:2014-05-14

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Abstract: 【Objective】The objective of this study is to identify ovine retinoic acid receptor gamma (RARG) gene and to discover the differential expression of ovine RARG in ovary at different estrous cycles. 【Method】 Eight one-year old Gansu alpine fine-wool sheep were chosen for estrus synchronization treatment and then divided into 2 groups based on the ovarian physiological situation: one is corpus luteum group and the other one is estrous group. RNA were isolated from the ovarian tissue and RT-PCR was used to clone the ovine RARG gene, bioinformation method was used to construct ovine RARG gene phylogenetic tree as well as the prediction of protein structure and potential function. Q-PCR was applied to discover the differential expression of ovine RARG in ovary at different estrous cycles. 【Result】 A 1 588 bp full-length ovine RARG cDNA was identified and contained a 1 377 bp predicted ORF (GenBank accession No: KF019682), which encoded 458 amino acids with a calculated molecular weight of 50.96 kDa and an estimated isoelectric point (pI) of 7.45. Phylogenetic tree analysis showed that the ovine RARG gene has a close relationship with Bos taurus’, and the gene sequence among these species contained high homologous. Protein structure prediction indicated that the ovine RARG protein contains two main domains: c4 zinc finger in nuclear hormone receptors(ZnF_C4), and ligand binding domain of hormone receptors (HOLI). Q-PCR result demonstrated that the expression of ovine RARG mRNA in estrous situation is higher than that in corpus luteum stage. 【Conclusion】 The ovine RARG gene may work as an important transcriptional factor involved in female ovine estrous cycle control.

Key words: sheep (Ovis aries) , estrous cycle , ovary , RARG , expression

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