Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (15): 3069-3076.doi: 10.3864/j.issn.0578-1752.2014.15.016

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Study on Differential Expression of Ovine RARG in Ovary at Different Estrous Cycles

 WANG  Wei-Min, HU  Ting-Xi, LI  Fa-Di, MA  You-Ji, FAN  Hong-Ying, PAN  Xiang-Yu   

  1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070
  • Received:2013-07-08 Online:2014-08-01 Published:2014-05-14

Abstract: 【Objective】The objective of this study is to identify ovine retinoic acid receptor gamma (RARG) gene and to discover the differential expression of ovine RARG in ovary at different estrous cycles. 【Method】 Eight one-year old Gansu alpine fine-wool sheep were chosen for estrus synchronization treatment and then divided into 2 groups based on the ovarian physiological situation: one is corpus luteum group and the other one is estrous group. RNA were isolated from the ovarian tissue and RT-PCR was used to clone the ovine RARG gene, bioinformation method was used to construct ovine RARG gene phylogenetic tree as well as the prediction of protein structure and potential function. Q-PCR was applied to discover the differential expression of ovine RARG in ovary at different estrous cycles. 【Result】 A 1 588 bp full-length ovine RARG cDNA was identified and contained a 1 377 bp predicted ORF (GenBank accession No: KF019682), which encoded 458 amino acids with a calculated molecular weight of 50.96 kDa and an estimated isoelectric point (pI) of 7.45. Phylogenetic tree analysis showed that the ovine RARG gene has a close relationship with Bos taurus’, and the gene sequence among these species contained high homologous. Protein structure prediction indicated that the ovine RARG protein contains two main domains: c4 zinc finger in nuclear hormone receptors(ZnF_C4), and ligand binding domain of hormone receptors (HOLI). Q-PCR result demonstrated that the expression of ovine RARG mRNA in estrous situation is higher than that in corpus luteum stage. 【Conclusion】 The ovine RARG gene may work as an important transcriptional factor involved in female ovine estrous cycle control.

Key words: sheep (Ovis aries) , estrous cycle , ovary , RARG , expression

[1]王锋. 动物繁殖学. 北京: 中国农业大学出版社, 2012.

Wang F. Animal Reproduction. Beijing: China Agricultural University Press, 2012. (in Chinese)

[2]Wirtanen L, Seguin C. Cloning of cDNAs encoding retinoic acid receptors RARγl,RARγ2,and a new splicing variant,RARγ3,from Aambystoma mexicanum and characterization of their expression during early development. Biochimica et Biophysica Acta, 2000, 1492(1): 8l-93.

[3]De Luca L M. Retinoids and their receptors in differentiation, embryogenesis, and neoplasia. The FASEB Journal, 1991, 5(14): 2924-2933.

[4]Raverdeau M, Gely-Pernot A, Féret B, Dennefeld C, Benoit G, Davidson I, Chambon P, Mark M, Ghyselinck N B. Retinoic acid induces Sertoli cell paracrine signals for spermatogonia differentiation but cell autonomously drives spermatocyte meiosis. Proceedings of the National Academy of Sciences of USA, 2012, 109(41): 16582-16587.

[5]Ma Z, Guo W, Niu H J, Yang F, Wang R W, Jiang Y G, Zhao Y P. Transcriptome network analysis reveals potential candidate genes for esophageal squamous cell carcinoma. Asian Pacific Journal of Cancer Prevention, 2012, 13: 767-773.

[6]Leid M, Kastner P, Durand B, Krust A, Leroy P, Lyons R, Mendelsohn C, Nagpal S, Nakshatri H, Reibel C, Saunders M, Chambon P. Retinoic acid signal transduction pathway.  Annals of The New York Academy of Sciences, 1993, 684: 19-34.

[7]Linney E. Retinoic acid receptors: transcription factors modulating gene regulation, development, and differentiation. Current Topics in Developmental Biology, 1992, 27: 309-350.

[8]Krust A, Kastner P, Petkovich M, Zelent A, Chambon P. A third human retinoic acid receptor, hRAR-gamma. Proceedings of the National Academy of Sciences of USA, 1989, 86(14): 5310-5314.

[9]Messer L, Wang L, Yelich J, Pomp D, Geisert R, Rothschild MF. Linkage mapping of the retinoic acid receptor-γ gene to porcine chromosome 5. Animal Genetics, 1996, 27(3): 175-177.

[10]Jenkins Z A, Henry H M, Galloway S M, Dodds K G, Montgomery G W. Cornparative linkage mapping of genes on sheep chromosome 3 provides evidence of chromosomal rearrangements in the evolution of the Bovidae. Cytogenetics and Cell Genetics, 1997, 78(3/4): 272-274.

[11]Messer L, Wang L, Legauh C. Mapping and investigation of candidate genes for litter size in French Large White pigs. Animal Genetic, 1996, 27: 114.

[12]郭晓红, 储明星, 周忠孝, 方丽, 叶素成. 小尾寒羊高繁殖力候选基因RARG的研究. 畜牧兽医学报, 2006, 37(8): 756-760.

Guo X H, Chu M X, Zhou Z X, Fang L, Ye S C. Study on RARG as a candidate gene for prolificacy of small Tailed Han sheep. Acta Veterinaria et Zoot echnica Sinica, 2006,37(8):756-760.(in Chinese)

[13]Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods, 2001, 25: 402-408.

[14]Klug A. Zinc finger peptides for the regulation of gene expression. Journal of Molecular Biology, 1999, 293(2): 215-218.

[15]Hall T M. Multiple modes of RNA recognition by zinc finger proteins. Current Opinion in Structural Biology, 2005, 15(3): 367-373.

[16]Brown R S. Zinc finger proteins: getting a grip on RNA. Current Opinion in Structural Biology, 2005, 15(1): 94-98.

[17]Gamsjaeger R, Liew C K, Loughlin F E, Crossley M, Mackay J P. Sticky fingers: zinc-fingers as protein-recognition motifs. Trends in Biochemical Sciences, 2007, 32(2): 63-70.

[18]Matthews J M, Sunde M. Zinc fingers-folds for many occasions. IUBMB Life, 2002, 54(6): 351-355.

[19]Laity J H, Lee B M, Wright P E. Zinc finger proteins: new insights into structural and functional diversity. Current Opinion in Structural Biology, 2001, 11(1): 39-46.

[20]Edwards D P. The role of coactivators and corepressors in the biology and mechanism of action of steroid hormone receptors. Journal of Mammary Gland Biology and Neoplasia, 2000, 5(3): 307-324.

[21]Bledsoe R K, Stewart E L, Pearce K H. Structure and function of the glucocorticoid receptor ligand binding domain. Vitamins and Hormones, 2004, 68:49-91.

[22]Gely-Pernot A, Raverdeau M, Célébi C, Dennefeld C, Feret B, Klopfenstein M, Yoshida S, Ghyselinck N B, Mark M. Spermatogonia differentiation requires retinoic acid receptor γ. Endocrinology, 2012, 153(1): 438-449.

[23]Wang W, Yang J, Liu H, Lu D, Chen X, Zenonos Z, Campos L S, Rad R, Guo G, Zhang S, Bradley A, Liu P. Rapid and efficient reprogramming of somatic cells to induced pluripotent stem cells by retinoic acid receptor gamma and liver receptor homolog 1. Proceedings of the National Academy of Sciences of USA, 2011, 108(45): 18283-18288.

[24]Jacobs H, Dennefeld C, Féret B, Viluksela M, Håkansson H, Mark M, Ghyselinck N B. Retinoic acid drives aryl hydrocarbon receptor expression and is instrumental to dioxin-induced toxicity during palate development. Environ Health Perspect, 2011, 119(11): 1590-1595.

[25]Jäger M, Ott C E, Grünhagen J, Hecht J, Schell H, Mundlos S, Duda G N, Robinson P N, Lienau J. Composite transcriptome assembly of RNA-seq data in a sheep model for delayed bone healing. BMC Genomics, 2011, 12:158.
[1] SHEN LongXian, WANG LiTing, HE Ke, DU Xue, YAN FeiFei, CHEN WeiHu, LÜ YaoPing, WANG Han, ZHOU XiaoLong, ZHAO AYong. Effects of Melatonin and Nicotinamide Mononucleotides on Proliferation of Skeletal Muscle Satellite Cells in Goose [J]. Scientia Agricultura Sinica, 2023, 56(2): 391-404.
[2] GU LiDan,LIU Yang,LI FangXiang,CHENG WeiNing. Cloning of Small Heat Shock Protein Gene Hsp21.9 in Sitodiplosis mosellana and Its Expression Characteristics During Diapause and Under Temperature Stresses [J]. Scientia Agricultura Sinica, 2023, 56(1): 79-89.
[3] ZHANG KeKun,CHEN KeQin,LI WanPing,QIAO HaoRong,ZHANG JunXia,LIU FengZhi,FANG YuLin,WANG HaiBo. Effects of Irrigation Amount on Berry Development and Aroma Components Accumulation of Shine Muscat Grape in Root-Restricted Cultivation [J]. Scientia Agricultura Sinica, 2023, 56(1): 129-143.
[4] MO WenJing,ZHU JiaWei,HE XinHua,YU HaiXia,JIANG HaiLing,QIN LiuFei,ZHANG YiLi,LI YuZe,LUO Cong. Functional Analysis of MiZAT10A and MiZAT10B Genes in Mango [J]. Scientia Agricultura Sinica, 2023, 56(1): 193-202.
[5] LI ShiJia,LÜ ZiJing,ZHAO Jin. Identification of R2R3-MYB Subfamily in Chinese Jujube and Their Expression Pattern During the Fruit Development [J]. Scientia Agricultura Sinica, 2022, 55(6): 1199-1212.
[6] LAI ChunWang, ZHOU XiaoJuan, CHEN Yan, LIU MengYu, XUE XiaoDong, XIAO XueChen, LIN WenZhong, LAI ZhongXiong, LIN YuLing. Identification of Ethylene Synthesis Pathway Genes in Longan and Its Response to ACC Treatment [J]. Scientia Agricultura Sinica, 2022, 55(3): 558-574.
[7] SHU JingTing,SHAN YanJu,JI GaiGe,ZHANG Ming,TU YunJie,LIU YiFan,JU XiaoJun,SHENG ZhongWei,TANG YanFei,LI Hua,ZOU JianMin. Relationship Between Expression Levels of Guangxi Partridge Chicken m6A Methyltransferase Genes, Myofiber Types and Myogenic Differentiation [J]. Scientia Agricultura Sinica, 2022, 55(3): 589-601.
[8] ZHAO HuiTing,PENG Zhu,JIANG YuSuo,ZHAO ShuGuo,HUANG Li,DU YaLi,GUO LiNa. Expression and Binding Properties of Odorant Binding Protein AcerOBP7 in Apis cerana cerana [J]. Scientia Agricultura Sinica, 2022, 55(3): 613-624.
[9] LI YuZe,ZHU JiaWei,LIN Wei,LAN MoYing,XIA LiMing,ZHANG YiLi,LUO Cong,HUANG Gui Xiang,HE XinHua. Cloning and Interaction Protein Screening of RHF2A Gene from Xiangshui Lemon [J]. Scientia Agricultura Sinica, 2022, 55(24): 4912-4926.
[10] GUO ShaoLei,XU JianLan,WANG XiaoJun,SU ZiWen,ZHANG BinBin,MA RuiJuan,YU MingLiang. Genome-Wide Identification and Expression Analysis of XTH Gene Family in Peach Fruit During Storage [J]. Scientia Agricultura Sinica, 2022, 55(23): 4702-4716.
[11] ZHANG Qi,DUAN Yu,SU Yue,JIANG QiQi,WANG ChunQing,BIN Yu,SONG Zhen. Construction and Application of Expression Vector Based on Citrus Leaf Blotch Virus [J]. Scientia Agricultura Sinica, 2022, 55(22): 4398-4407.
[12] HAO Yan,LI XiaoYing,YE Mao,LIU YaTing,WANG TianYu,WANG HaiJing,ZHANG LiBin,XIAO Xiao,WU JunKai. Characteristics of Volatile Components in Peach Fruits of 21shiji and Jiucui and Their Hybrid Progenies [J]. Scientia Agricultura Sinica, 2022, 55(22): 4487-4499.
[13] KANG Chen,ZHAO XueFang,LI YaDong,TIAN ZheJuan,WANG Peng,WU ZhiMing. Genome-Wide Identification and Analysis of CC-NBS-LRR Family in Response to Downy Mildew and Powdery Mildew in Cucumis sativus [J]. Scientia Agricultura Sinica, 2022, 55(19): 3751-3766.
[14] YuXia WEN,Jian ZHANG,Qin WANG,Jing WANG,YueHong PEI,ShaoRui TIAN,GuangJin FAN,XiaoZhou MA,XianChao SUN. Cloning, Expression and Anti-TMV Function Analysis of Nicotiana benthamiana NbMBF1c [J]. Scientia Agricultura Sinica, 2022, 55(18): 3543-3555.
[15] ZHANG YunXiu,JIANG Xu,WEI ChunXue,JIANG XueQian,LU DongYu,LONG RuiCai,YANG QingChuan,WANG Zhen,KANG JunMei. The Functional Analysis of High Mobility Group MsHMG-Y Involved in Flowering Regulation in Medicago sativa L. [J]. Scientia Agricultura Sinica, 2022, 55(16): 3082-3092.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!