Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (22): 4745-4752.doi: 10.3864/j.issn.0578-1752.2013.22.012

• HORTICULTURE • Previous Articles     Next Articles

Control of Exogenous H2O2 on Dry Rot of Potato Tuber and Possible Mechanism of Action

 HU  Lin-Gang, LI  Jian-Peng, LI  Yong-Cai, BI  Yang, GE  Yong-Hong, WANG  Yi   

  1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070
  • Received:2013-04-03 Online:2013-11-15 Published:2013-05-23

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Abstract: 【Objective】The inhibitory effect of exogenous H2O2 against dry rot of potato and its mechanism of action were studied in this paper. 【Method】Mycelium growth, colonial morphology and hyphae ultrastructure of Fusarium sulphureum after treated with H2O2 were observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) in vitro test, and the curative and preventative effect of exogenous H2O2 on dry rot of potato tuber (Longshu No.3) inoculated with F. sulphureum pre- and post-etreatment were also evaluated.【Result】Spore germination and mycelium growth of F. sulphureum were strongly inhibited by exogenous H2O2 in a concentration-dependent manner. Besides, exogenous H2O2 could dramatically increase the membrane permability of F. sulphureum. Morphological changes such as asymmetrical, distorted, broken and wizened hyphae were observed by scanning electron microscopy (SEM) observation. Transmission electron microscopy (TEM) observation further documented these changes, irregularly thickened hypha cell walls and cavity could be observed. Results of in vivo test showed that exogenous H2O2 was effective in the control of dry rot of potato tuber inoculated with F. sulphureum pre- and post-etreatment.【Conclusion】 H2O2 can directly suppress the pathogen, and effectively increase the disease resistance of potato tuber tissue, thus it represent a potential fungicides for the integrated control of postharvest diseases in potato tuber.

Key words: potato , dry rot , H2O2 , antifungal activity , induced disease resistance

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