Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (20): 4197-4209.doi: 10.3864/j.issn.0578-1752.2013.20.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Diversity Research of Mungbean Germplasm Resources by SSR Markers in China

 LIU  Yan, CHENG  Xu-Zhen, WANG  Li-Xia, WANG  Su-Hua, BAI  Peng, WU  Chuan-Shu   

  1. Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081
  • Received:2013-04-10 Online:2013-10-15 Published:2013-07-08

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Abstract: 【Objective】The purpose of this research was to investigate the genetic diversity, relationship and differentiation of cultivated mungbean germplasm resources in China, and provide useful information on effective utilization of resources, new genes mining and breeding.【Method】The genetic diversity of 18 mungbean groups from different areas was analyzed by using 40 SSR markers.【Result】A total of 125 alleles were identified with an average of 3.1 alleles per locus. The average of effective number of alleles (NE), Nei's gene diversity (H), polymorphism information content(PIC), expected heterozygosity (He), and Shannon’s information index (I) were 1.8, 0.4233, 0.3497, 0.4241, and 0.6754, respectively. Hebei, Shandong and Anhui provinces had a higher genetic variation than other provinces. The average observed heterozygosity (Ho) was 0.1001 and the average of inbreeding coefficient among population(Fis) was 0.6759, indicating that a certain degree of inbreeding existed in Chinese mungbean germplasm. The gene flow (Nm= 0.6936) and the average genetic differentiation (Fst= 0.2649) also showed a high genetic variation within Chinese mungbean. A total of 272 individuals were divided into 2 groups and 18 populations were divided into 3 groups based on Popgene software. The genetic distances between groups were agreeable with their geographical origin.【Conclusion】There was a high genetic diversity level within Chinese mungbean germplasm. Geographical environment and ecological factors affect the genetic differentiation among different populations.

Key words: mungbean , SSR , genetic diversity , genetic relationship

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