Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (15): 3220-3226.doi: 10.3864/j.issn.0578-1752.2013.15.017

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Analysis of Factors Affecting the Activity of AI-2 of Avian Pathogenic Escherichia coli

 BAI  Hao-1, 2 , HAN  Xian-Gan-1, LIU  Lei-1, QI  Ke-Zong-2, LIU  Hai-Wen-1, DING  Chan-1, YU  Sheng-Qing-1   

  1. 1.Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241
    2.College of Animal Science, Anhui Agricultural University, Hefei 230036
  • Received:2012-12-17 Online:2013-08-01 Published:2013-06-08

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Abstract: 【Objective】 In this study, the relationship between the transcription level of luxS and pfs and AI-2 production in different growth phases and culture conditions were analyzed. 【Method】 AI-2 activity in different growth phases and culture conditions was measured using the V. harveyi bioluminescence assay.The levels of luxS and pfs mRNA were analyzed using real-time PCR. 【Result】 The results showed that the level of AI-2 was consistent with the level of luxS mRNA in different growth phases. AI-2 production and luxS mRNA by APEC was increased by supplementation of glucose, maltose, and NaCl, while the addition of sucrose was decreased. The transcription level of luxS was correlated to the level of AI-2 production, while the transcription level of pfs did not correlate with the level of AI-2 production. 【Conclusion】 The level of transcription of luxS is highly correlated with the level of AI-2 production,while the level of transcription of pfs does not correlate with the level of AI-2 production. Glucose, maltose and NaCl accelerate the synthesis of AI-2.

Key words: avian pathogenic Escherichia coli , autoindure-2 , luxS , pfs , real-time PCR

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