Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (10): 2103-2109.doi: 10.3864/j.issn.0578-1752.2013.10.016

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Expression and Localization of Claudin-11 in Adult Alpaca Ovary

 GENG  Jian-Jun, GUO  Qing-Yun, PANG  Ya-Miao, BAI  Jun-Ming, HE  Jun-Ping   

  1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, Shanxi
  • Received:2013-01-07 Online:2013-05-15 Published:2013-04-07

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Abstract: 【Objective】To explore the mechanism of Claudin-11 in promoting the follicular development and regulating the ovulation, an experiment was carried out to investigate the expression and localization of Claudin-11 in adult alpaca ovary. 【Method】 Using the adult female alpaca as the object of study, the expression of Claudin-11 in the ovary was analyzed by RT-PCR and real-time PCR. Immunohistochemistry and western blotting were used to explore the localization of Claudin-11 in alpaca ovary.【Result】The expression of Claudin-11 in ovarian primary follicle was weaker than in the secondary follicle. Polyclonal antibody happened specific immune response with the crude extract from the ovarian. The expression of Claudin-11 in ovarian primary follicle and secondary follicle was weaker than that in flat or cube follicle cell cytoplasm. Claudin-11 was mainly located in the granulosa cell of the secondary follicle endothecium.【Conclusion】The results showed that Claudin-11 expressed in adult alpaca ovary, and there was a specific cellular localization.

Key words: ovary , follicular development , Claudin-11 , expression , localization

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