Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (8): 1564-1570.doi: 10.3864/j.issn.0578-1752.2013.08.005

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY • Previous Articles     Next Articles

Research on Carbon Metabolism Characteristics of Transgenic Bt Rice

 WANG  Wei-Bo, CUI  Hai-Rui, LU  Mei-Zhen, SHU  Qing-Yao, SHEN  Sheng-Quan   

  1. 1.Institute of Nuclear-Agricultural Sciences, Zhejiang University/Key Laboratory of Nuclear Agricultural Sciences, Ministry of Agriculture, Hangzhou 310029
    2.College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310032
  • Received:2012-10-08 Online:2013-04-15 Published:2012-11-20

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Abstract: 【Objective】The objective of this study was to evaluate the effect of transgene Cry1Ab on carbon metabolism characteristics in rice. 【Method】Transgenic Bt (Cry1Ab) rice KMD1 and its parent Xiushui 11, as well as three pairs of Bt and non-Bt rice near-isogenic lines which were derived from the progeny of crosses and continuous backcrosses between the KMD1 and Minghui 63, R3027 and 99Ya162, respectively, were field grown and measured for the content of chlorophyll and soluble sugar, the activity of RuBPCase, sucrose synthetase and sucrose phosphate synthase in leaves, the dry weight and organic carbon accumulation per plant at different developmental stages.【Result】At tillering stage, KMD 1 had significant lower contents of chlorophyll a, chlorophyll b and soluble sugar, lower activities of sucrose synthetase and sucrose phosphate synthase, and lower dry weight and organic carbon accumulation than those of Xiushui 11, but no significant differences between other 3 pairs of Bt and non-Bt rice lines were observed in all physiological indexes tested. At full heading stage, the activity of sucrose phosphate synthase of KMD1 was significantly higher than that of Xiushui 11, although its dry weight and organic carbon accumulation were significantly lower, while Bt R3027 showed a significant lower sucrose synthetase activity and Bt 99Ya162 showed significant higher soluble sugar content and sucrose phosphate synthase activity than the non-Bt type, respectively. At maturity stage, no significant differences were found in dry weight and organic carbon accumulation in four pairs of Bt and non-Bt rice lines. 【Conclusion】Significant change of most physiological indexes related to carbon metabolism in transgenic rice KMD 1 was resulted from somaclonal variations, while the effect of Cry1Ab gene on individual index in other 3 pairs of Bt and non-Bt rice near-isogenic lines was transitory and related to the genetic background of Bt gene incorporation.

Key words: rice , Bt gene , carbon metabolism , physiological indexes

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