Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (5): 943-949.doi: 10.3864/j.issn.0578-1752.2013.05.009

• PLANT PROTECTION • Previous Articles     Next Articles

SCAR Molecular Markers Correlated with Populations of Meloidogyne incognita Virulent to Resistance Gene Me3

 WANG  Gang, LI  二Feng, MAO  Zhen-Chuan, XIE  Bing-Yan, FENG  Dong-Xin   

  1. Key Laboratory of Horticultural Crops Biology and Genetic Improvement, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081
  • Received:2012-09-10 Online:2013-03-01 Published:2012-12-21

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Abstract: 【Objective】Molecular markers of virulent populations against Me3 in Meloidogyne incognita was studied in order to detect the virulence mutation rapidly and effectively. 【Method】Root-knot nematode populations including avirulent population, populations overcoming resistant gene Me3 and the mixed group of the two population were used as experiment materials, polymerase chain reaction was done with 100 primer pairs designed according to M. incognita genome and 19 pairs reported in literature to screen specific band among three populations. And subsequently SCAR primers were designed and a multiplex PCR reaction system was built. 【Result】Seven primer pairs amplifying stability bands were screened, two of which were converted into SCAR markers differentiating the three populations. Multiplex PCR from avirulent population and Me3-virulent isolates generated a fragment of 999 and 629 bp, respectively, while from the mixed group generated both of the above fragments. 【Conclusion】Virulent mutation markers were successfully developed in M. incognita, and one-step multiplex PCR can be used for identification of Me3-virulence.

Key words: Meloidogyne incognita , molecular marker , SCAR , virulence mutation

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