Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (13): 2628-2635.doi: 10.3864/j.issn.0578-1752.2012.13.007

• PLANT PROTECTION • Previous Articles     Next Articles

Effects of Cuminic Acid on the Growth and Development of Phytophthora capsici Leonian

 FENG  Jun-Tao, HAN  Li-Rong, FAN  Rui-Juan, CHEN  Cong-Zhen, ZHANG  Xing   

  1. 西北农林科技大学植物保护学院/无公害农药研究服务中心，陕西杨凌 712100
  • Received:2011-08-28 Online:2012-07-01 Published:2012-03-12

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Abstract: 【Objective】 The objective of this study is to explore the activity of cuminic acid against the growth of Phytophthora capsici.【Method】Agar plate tests, pendant-drop method and pot experiments were conducted to test the inhibitory effect on P. capsici in vitro and in vivo of cuminic acid. The ultrastructure of P. capsici treated with cuminic acid was observed through scanning electron microscope (SEM) and transmission electron microscope (TEM). And the distribution of the main components, β-1,3-glucan content and β-1,3-glucanase activity of the P. capsici cell wall exposed to cuminic acid were studied using Calcofluor white staining and phenol sulfuric acid method. 【Result】 Cuminic acid displayed strong antifungal activity to P. capsici in vitro and in vivo, and the germination of zoospores was strongly inhibited. The observations with SEM revealed that the excessive branching mycelium occurred, the surface of hyphae was tumorous protuberance, irregular swelling and intensive growing point. The sporangiums were invaginated, and some of them even collapsed and formed shells. The observations with TEM showed that the hyphal cell wall became thickening irregularly and the color was deepened. The mitochondria swelled, crest in some of them had disappeared, some small organelles such as endoplasmic reticulum and ribosomal were losing their structures. Moreover, the polar deposition of newly synthesized cell wall components in germinating cystospores was disturbed and the β-1,3-glucan content and β-1, 3-glucanase activity decreased significantly. 【Conclusion】 Cuminic acid significantly inhibited the growth and development of P. capsici, so it is worthy of further study.

Key words: cuminic acid, Phytophthora capsici, growth and development

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