Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (13): 2580-2591.doi: 10.3864/j.issn.0578-1752.2012.13.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Association Mapping of Sesame (Sesamum indicum L.) Resistance to Macrophomina phaseolina and Identification of Resistant Accessions

 ZHANG  Yan-Xin, WANG  Lin-Hai, LI  Dong-Hua, WEI  Wen-Liang, GAO  Yuan, ZHANG  Xiu-Rong   

  1. 中国农业科学院油料作物研究所/农业部油料作物生物学与遗传育种重点实验室，武汉 430062
  • Received:2012-01-20 Online:2012-07-01 Published:2012-03-23

PDF

891

Cited

2

Abstract: 【Objective】Developing molecular markers related with resistance to Macrophomina phaseolina from natural population, identifying elite resistant accessions. 【Method】In this study, 216 sesame core collections were scanned by 79 pairs of EST-SSR, SRAP and AFLP primers, accurate identification of their resistance to Macrophomina phaseolina was carried out from 2009 to 2011, and based on these data the genes related with resistance to M. phaseolina were located by association analysis. 【Result】A total of 608 polymorphic bands were amplified, population structure analysis indicated that it was consisted of 2 subgroups, 43 markers significantly associated with M. phaseolina disease index simultaneously in 2 or 3 years were detected by association analysis based on GLM(Q) and MLM(Q+K) model, explained 1.82% to 9.46% of phenotypic variation, 3 associated markers were detected by both of 2 models, and marker M7E6-1 was presented simultaneously in 3 years. Ten accessions with resistance to M. phaseolina were identified, the coincident rate of 43 associated markers in the 10 accessions was 60% to 100%, with an average of 92.09%. 【Conclusion】 The tested population was consisted of 2 subgroups. There was no relationship between division of subgroup and geographical origin of the material, 43 markers were detected significantly and stably associated with M. phaseolina disease index. There was a diverse variation among the resistance to M. phaseolina of the tested population, and 10 accessions with stable resistance to M. phaseolina were identified.

Key words: sesame, Macrophomina phaseolina, association mapping, resistant accession, identification

[1]钟  乃. 芝麻高产栽培. 北京: 金盾出版社, 1991.

Zhong N. Sesame Planting with High Yield. Beijing: Golden Shield Press, 1991. (in Chinese)

[2]中国农作物病虫害编辑委员会. 中国农作物病虫害: 上册. 北京: 农业出版社, 1979.

Editorial Board of Crop Pests and Diseases. Crop Pests and Diseases: Volume I. Beijing: Agriculture Press, 1979. (in Chinese)

[3]李丽丽. 世界芝麻病害研究进展. 中国油料, 1993, 2: 75-77.

Li L L. Progress on world sesame disease research. Oil Crops in China, 1993, 2: 75-77. (in Chinese)

[4]杨修身, 杨永东, 薛香云, 靳秀兰. 芝麻叶斑病、茎点枯病的发生对产量的影响. 河南农业科学, 1990, 6: 15-17.

Yang X S, Yang Y D, Xue X Y, Jin X L. Occurrence of the sesame leaf spot, charcoal rot impact on yield. Journal of Henan Agricultural Sciences, 1990, 6: 15 -17. (in Chinese)

[5]吴桂香, 李运良, 刘世扬, 王建华, 白青牛. 芝麻茎点枯病的发生规律及综合防治技术. 河南农业科学, 1996(8): 17-18.

Wu G X, Li Y L, Liu S Y, Wang J H, Bai Q N. Occurrence and integrated control technology of sesame charcoal rot. Journal of Henan Agricultural Sciences, 1996(8): 17-18. (in Chinese)

[6]潘正安, 余安安, 蔡德君, 李灵贵, 洪海林. 芝麻病害的发生特点与防治技术. 湖北植保, 2008(2): 17-20.

Pan Z A, Yu A A, Cai D J, Li L G, Hong H L. Occurrence and control technology of sesame diseases. Hubei Plant Protection, 2008(2): 17-20. (in Chinese)

[7]李丽丽, 王圣玉, 方小平, 黄早花, 汪山涛, 李民良, 崔苗青. 我国芝麻种质资源抗茎点枯病鉴定. 中国油料, 1991(1): 3-6.

Li L L, Wang S Y, Fang X P, Huang Z H, Wang S T, Li M L, Cui M Q. Identification of sesame germplasm resources resistance to charcoal rot in China. Oil Crops in China, 1991(1): 3-6. (in Chinese)

[8]冯祥运, 张秀荣, 刘越英. 芝麻优良种质资源的深化鉴定与综合评价. 中国油料, 1996, 18(3): 63-66.

Feng X Y, Zhang X R, Liu Y Y. Deepen identification and comprehensive evaluation of elite sesame germplasm resources. Oil Crops in China, 1996, 18(3): 63-66. (in Chinese)

[9]崔苗青, 李义芝, 高新国, 韩玉枝. 芝麻种质资源抗茎点枯病鉴定与评价. 作物品种资源, 1999(2): 36-37.

Cui M Q, Li Y Z, Gao X G, Han Y Z. Identification and evaluation of sesame germplasm resources resistance to charcoal rot. Crop Germplasm Resources, 1999(2): 36 -37. (in Chinese)

[10]张秀荣, 程  勇, 刘胜毅, 冯祥运, 靳利民, 靳巧玲, 黄翠英, 王自安, 徐新福, 孙梅英. 芝麻抗茎点枯病及枯萎病种质的评价. 中国油料作物学报, 2001, 23(4): 23-27.

Zhang X R, Cheng Y, Liu S Y, Feng X Y, Jin L M, Jin Q L, Huang C Y, Wang Z A, Xu X F, Sun M Y. Evaluation of sesame germplasm resistance to Macrophomina phaseolina and Fusarium oxysporium. Chinese Journal of Oil Crop Sciences, 2001, 23(4): 23-27. (in Chinese)

[11]Reddy P S, Fakrudin B, Punnuri S M, Arun S S, Kuruvinashetti M S, Das I K, Seetharama N. Molecular mapping of genomic regions harboring QTLs for stalk rot resistance in sorghum. Euphytica, 2008, 159: 191-198.

[12]Thornsberry J M, Goodman M M, Doebley J, Kresovich S, Nielsen D, Buckler E S. Dwarf8 polymorphisms associate with variation in flowering time. Nature Genetics, 2001, 28: 286-289.

[13]Nordborg M, Borevitz J O, Bergelson J, Berry C C, Chory J, Hagenblad J, Kreitman M, Maloof J N, Noyes T, Oefner P J, Stahl E A, Weigel D. The extent of linkage disequilibrium in Arabidopsis thaliana. Nature Genetics, 2002, 30: 190-193.

[14]Hansen M, Kraft T, Ganestam S, Säll T, Nilsson N O. Linkage disequilibrium mapping of the bolting gene in sea beet using AFLP markers. Genetics Research, 2001, 77: 61-66.

[15]Kraakman A T W, Niks R E, Van den Berg P M M M, Stam P, Van Eeuwijk F A. Linkage disequilibrium mapping of yield and yield stability in modern spring barley cultivars. Genetics, 2004, 168: 435-446.

[16]Achleitner A, Tinker N A, Zechner E, Buerstmayr H. Genetic diversity among oat varieties of worldwide origin and associations of AFLP markers with quantitative traits. Theoretical and Applied Genetics, 2008,117: 1041-1053.

[17]Agrama1 H A, Eizenga G C, Yan W. Association mapping of yield and its components in rice cultivars. Molecular Breeding, 2007, 19: 341-356.

[18]Tommasini L, Schnurbusch T, Fossati D, Mascher F, Keller B. Association mapping of Stagonospora nodorum blotch resistance in modern European winter wheat varieties. Theoretical and Applied Genetics, 2007, 115: 697-708.

[19]Peng J H, Bai Y, Haley S D, Lapitan N L V. Microsatellite-based molecular diversity of bread wheat germplasm and association mapping of wheat resistance to the Russian wheat aphid. Genetica, 2009, 135(1): 95-122.

[20]张  军, 赵团结, 盖钧镒. 大豆育成品种农艺性状QTL与SSR标记的关联分析. 作物学报, 2008, 34(12): 2059-2069.

Zhang J, Zhao T J, Gai J Y. Association analysis of agronomic trait QTLs with SSR markers in released soybean cultivars. Acta Agronomica Sinica, 2008, 34(12): 2059-2069. (in Chinese)

[21]贺道华, 邢宏宜, 赵俊兴, 李婷婷, 汤  益, 曾  舟. 棉花资源群体结构的推测与纤维品质的关联分析. 西北农林科技大学学报: 自然科学版, 2011, 39(1): 103-112.

He D H, Xing H Y, Zhao J X, Li T T, Tang Y, Zeng Z. Population structure inferring and association analysis of fiber quality in cultivated cotton. Journal of Northwest Agriculture and Forestry University: Natural Science Edition, 2011, 39(1): 103-112. (in Chinese)

[22]赵  波, 叶  剑, 金文林, 曾潮武, 吴宝美, 濮绍京, 潘金豹, 万  平. 不同类型小豆种质SSR标记遗传多样性及性状关联分析. 中国农业科学, 2011, 44(4): 673-682.

Zhao B, Ye J, Jin W L, Zeng C W, Wu B M, Pu S J, Pan J B, Wan P. Analysis on genetic diversity and trait association of different types of Azuki bean (Vigna angularisi) by SSR markers. Scientia Agricultura Sinica, 2011, 44(4): 673 - 682. (in Chinese)

[23]Zhang J A, Hao C Y, Ren Q, Chang X P, Liu G R, Jing R L. Association mapping of dynamic developmental plant height in common wheat. Planta, 2011, 234(5): 891-902.

[24]Zhao W, Park E J, Chung J W, Park Y J, Chung I M, Ahn J K, Kim G H. Association analysis of the amino acid contents in rice. Journal of Integrative Plant Biology, 2009, 51(12): 1126-1137.

[25]Shao Y F, Jin L, Zhang G, Lu Y, Shen Y, Bao J S. Association mapping of grain color, phenolic content, flavonoid content and antioxidant capacity in dehulled rice. Theoretical and Applied Genetics, 2011, 122: 1005-1016.

[26]Shi C, Navabi A, Yu K F. Association mapping of common bacterial blight resistance QTL in Ontario bean breeding populations. BMC Plant Biology, 2011, 11: 52-62.

[27]Jestin C, Lode M, Vallée P, Domin C, Falentin C, Horvais R, Coedel S, Manzanares-Dauleux M J, Delourme R. Association mapping of quantitative resistance for Leptosphaeria maculans in oilseed rape (Brassica napus L.). Molecular Breeding, 2011, 27: 271-287.

[28]杨小红, 严建兵, 郑艳萍, 余建明, 李建生. 植物数量性状关联分析研究进展. 作物学报, 2007, 33(4): 523-530.

Yang X H, Yan J B, Zheng Y P, Yu J M, Li J S. Reviews of association analysis for quantitative traits in plants. Acta Agronomica Sinica, 2007, 33(4): 523-530. (in Chinese)

[29]Zhang X R, Zhao Y Z, Cheng Y, Feng X Y, Guo Q Y, Zhou M D, Hodgkin T. Establishment of sesame germplasm core collection in China. Genetic Resources and Crop Evolution, 2000, 47: 273-279.

[30]王林海, 黎冬华, 张艳欣, 黄俊斌, 张占英, 张晓燕, 张秀荣. 我国芝麻主产区茎点枯病病原菌生物学特性分析. 华北农学报, 2011, 26(4): 232-238.

Wang L H, Li D H, Zhang Y X, Huang J B, Zhang Z Y, Zhang X Y, Zhang X R. Biological characteristics of the pathogen causing sesame charcoal rot from the main sesame production areas in China. Acta Agriculturae Boreali-Sinica, 2011, 26(4): 232-238. (in Chinese)

[31]张秀荣, 冯祥运. 芝麻种质资源描述规范和数据标准. 北京: 中国农业出版社, 2006.

Zhang X R, Feng X Y. Descriptors and Data Standard for Sesame (Sesamum indicum L.). Beijing: China Agriculture Press, 2006. (in Chinese)

[32]吕海霞, 张艳欣, 王林海, 张晓燕, 张秀荣. 芝麻DNA高效提取及PAGE快速银染方法. 中国农学通报,  2010, 26(15): 75-77.

Lü H X, Zhang Y X, Wang L H, Zhang X Y, Zhang X R. Methods for efficient extraction of sesame genomic DNA and rapid sliver staining of PAGE. Chinese Agricultural Science Bulletin, 2010, 26(15): 75-77. (in Chinese)

[33]Zhang Y X, Zhang X R, Hua W, Wang L H, Che Z. Analysis of genetic diversity among indigenous landraces from sesame (Sesamum indicum L.) core collection in China as revealed by SRAP and SSR markers. Genes and Genomics, 2010, 32: 207-215.

[34]孙  建, 涂玉琴, 张秀荣. 芝麻空间诱变品系（种）的DNA指纹分析. 中国农业科学, 2007, 40(12): 2696-2701.

Sun J, Tu Y Q, Zhang X R. DNA fingerprint analysis in space-induced sesame mutant lines. Scientia Agricultura Sinica, 2007, 40(12): 2696-2701. (in Chinese)

[35]张艳欣, 张秀荣, 车  卓, 王林海. 应用SRAP标记分析白芝麻核心种质遗传多样性. 中国油料作物学报, 2010, 32(1): 46-52.

Zhang Y X, Zhang X R, Che Z, Wang L H. Genetic diversity analysis of core collection of white coat sesame seed (Sesamum indicum L.) in China using SRAP markers. Chinese Journal of Oil Crop Sciences, 2010, 32(1): 46-52. (in Chinese)

[36]Yeh F C, Boyle T J B. Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belgian Journal of Botany, 1997, 129: 157.

[37]Bradbury P J, Zhang Z, Kroon D E, Casstevens T M, Ramdoss Y, Buckler E S. TASSEL: Software for association mapping of complex traits in diverse samples. Bioinformatics, 2007, 23: 2633-2635.

[38]Pritchard J K, Wen W. Documentation for STRUCTURE Software: Version 2.2. Chicago: The University of Chicago Press, 2007.

[39]唐启义, 冯明光. 实用统计分析及其计算机处理平台. 北京: 科学出版社, 2007.

Tang Q Y, Feng M G. Practical Statistics and Computer Processing Platform. Beijing: Science Press, 2007. (in Chinese)

[40]Li G, Quiros C F. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: Its application to mapping and gene tagging in Brassica. Theoretical and Applied Genetics, 2001, 103: 455-461.

[41]Yu J M, Pressoir G, Briggs W H, Bi I V, Yamasaki M, Doebley J F, McMullen M D, Gaut B S, Holland J B, Nielsen D, Holland J B, Kresovich S, Buckler E S. A unified mixed-model method for association mapping accounting for multiple levels of relatedness, Nature Genetics, 2005, 38: 203-208.

[42]Zhao K, Aranzana M J, Kim S, Lister C, Shindo C, Tang C, Toomajian C, Zheng H, Dean C, Marjoram P, Nordborg M. An Arabidopsis example of association mapping in structured samples, PLoS Genetics, 2007, 3(1): 0071-0082.

[43]Wang J, McClean P E, Lee R, Goos R J, Helms T. Association mapping of iron deficiency chlorosis loci in soybean (Glycine max L. Merr.) advanced breeding lines, Theoretical and Applied Genetics, 2008, 116(6): 777-787.
[1] SUN YaLing, WANG QingHua, SHU Rui, YUE LiXin, WANG ZhenBao, LI ZhaoXia, GAO LiMin, CHENG Hong, FU ZaiQiu, HUO YuMeng. Production and Characterization of Interspecific Hybrids Between Allium fistulosum and Allium przewalskianum Regel [J]. Scientia Agricultura Sinica, 2026, 59(3): 619-636.
[2] HE ZhiLin, SUN CuiXia, YUE HongLi, TAN YueXia, ZHANG YaoHai, WANG FuSheng, LIU SiTao, JIANG Dong. Genetic Diversity Analysis and GWAS of Alloocimene Based on Resequencing of Citron, Lemon Germplasm Resources [J]. Scientia Agricultura Sinica, 2026, 59(2): 386-401.
[3] XU DuoDuo, DU QianQian, ZHAO LiXiang, LI Yan, HUANG Gan, LI YongHua, LU JiuXing. Genome-Wide Analysis of AP2/ERF Transcription Factors in Peony [J]. Scientia Agricultura Sinica, 2025, 58(23): 5031-5045.
[4] ZHANG ZhiChun, HE XiaoYi, HE ChengJun, GU DongLin, ZHONG Lian, GOU Yan, GAO BiXing, GENG Zhao, LI XiaoHong, CHEN SiKai. Comparative Analysis of Volatile Oil Chemical Constituents from Zanthoxylum bungeanum Seeds in Yanyuan and Hanyuan Based on GC-MS Technology [J]. Scientia Agricultura Sinica, 2025, 58(23): 5057-5070.
[5] CAI HaiLin, HUANG XinRong, ZENG WeiAi, PENG ZhiXin, ZHAO AJuan, BO ChunBin, XIANG ShiPeng, GAO PengCheng, ZHANG ChengSheng, ZHAO DongLin. Structure and Herbicidal Activity of 3,5-Dimethylorsellinic Acid-Derived Meroterpenoids from Penicillium sclerotiorum HY5 [J]. Scientia Agricultura Sinica, 2025, 58(22): 4673-4687.
[6] SU XiaoMei, YANG ZongHui, LIU ShuMei, ZHANG ZongJie, LÜ HongJun, HOU LiXia. Development and Application of A KASP Marker-Based Identification System for Tomato Varieties [J]. Scientia Agricultura Sinica, 2025, 58(22): 4746-4756.
[7] WU ShuYu, HENG YanFang, YU TaiFei, WANG ShiJia, YU SiJia, LI Yuan, HU Zheng, ZHANG Hui, SUN XianJun, LI Liang, JIANG QiYan. Identification of Salt Tolerance in Maize Natural Populations at the Seedling Stage and Analysis of Salt Tolerance-Associated Genes [J]. Scientia Agricultura Sinica, 2025, 58(20): 4085-4099.
[8] CHEN YongXian, CHEN RuiJiang, DU YiZhi, ZHU JunJie, CHEN WanXia, ZHAO ZiHan, WANG JiChun, DU Kang, ZHANG Kai. Screening and Identification of Drought-Tolerant Sweet Potato Germplasm Resources [J]. Scientia Agricultura Sinica, 2025, 58(2): 214-237.
[9] PAN Yuan, WANG De, LIU Nan, MENG XiangLong, DAI PengBo, LI Bo, HU TongLe, WANG ShuTong, CAO KeQiang, WANG YaNan. Evaluation of the Effectiveness of Two High-Throughput Sequencing Techniques in Identifying Apple Viruses and Identification of Two Novel Viruses [J]. Scientia Agricultura Sinica, 2025, 58(2): 266-280.
[10] LUO ZiShu, ZHANG YiJia, ZHOU Rong, ZHANG YanXin, ZHOU Ting, YOU Jun, WANG LinHai. Characterization of Antioxidant Activities in Sesame Leaves and Screening of High Antioxidant Germplasm [J]. Scientia Agricultura Sinica, 2025, 58(19): 3814-3824.
[11] YI ZeHui, WANG Ying, SONG HuiXia, ZHAO Jing, MAO LiPing. Genome-Wide Identification and Expression Analysis of Peroxiredoxins Gene Family in Asparagus officinalis [J]. Scientia Agricultura Sinica, 2025, 58(18): 3728-3743.
[12] LI YaYu, WANG XinLiang, ZHOU JinHuan, LI ChuXin, LI JiaXin, TIAN XuBin, SONG Zhen. Construction and Infectivity Identification of Genome-Length cDNA of Citrus Psorosis Virus [J]. Scientia Agricultura Sinica, 2025, 58(17): 3451-3460.
[13] ZHAO YuMeng, LI GuangXu, DAI QiDong, LIU JiaCheng, ZHAO HaiJuan, ZHANG YuJun, YANG Hua, LIU Shuo. Isolation and Identification of Pathogen of Plum Bacterial Shot Hole and Laboratory Test of Bactericide Toxicity [J]. Scientia Agricultura Sinica, 2025, 58(12): 2358-2370.
[14] WEI NaiCui, TAO JinBo, YUAN MingYang, ZHANG Yu, KAI MengXiang, QIAO Ling, WU BangBang, HAO YuQiong, ZHENG XingWei, WANG JuanLing, ZHAO JiaJia, ZHENG Jun. Seedling Characterization and Genetic Analysis of Low Phosphorus Tolerance in Shanxi Varieties [J]. Scientia Agricultura Sinica, 2024, 57(5): 831-845.
[15] CHEN XiHong, CAI Wei, YU Yun, LI Min, WANG NianWu, DU ZhenGuo, SHEN JianGuo, GAO FangLuan. Identification of Tea Plant Viruses in Fujian Province and Establishment of Multiplex PCR Detection Assay [J]. Scientia Agricultura Sinica, 2024, 57(4): 698-710.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd