陆地棉干旱胁迫响应基因GhGR的克隆及特征分析

doi:10.3864/j.issn.0578-1752.2012.08.021

Abstract

Abstract: 【Objective】The objective of this study is to clone and make bioinformatics analysis of the sequence of the glutathione reductase gene from G. hirsutum L.【Method】The full length of GhGR was cloned by RACE and RT-PCR techniques，the gene sequence obtained and the putative amino acid sequence were analyzed by bioinformatics software. Its expression patterns were analyzed by real-time PCR and instantaneous expression. 【Result】The GhGR full length cDNA sequence contained 1 035 bp, and ORF was 792 bp, which encoded 263 amino acid residues. Bioinformatics analysis showed that GhGR had high similarity of 90%, 91% and 91% to Populus trichocarpa (XP_002299276.1), Ricinus communis (XP_002518118.1) and Vitis vinifera (CAN74593.1), respectively. Phylogenetic analysis showed that GhGR was much closer to Vitis vinifera, the expression protein of GhGR was located in the cell membrane and cell nucleus membrane, and its expression could be induced by drought.【Conclusion】The glutathione reductase gene GhGR was cloned for the first time from G. hirsutum L. The further studies of GhGR will have an important significance in drought- tolerance for cotton breeding.

Key words: Gossypium hirsutum L., GhGR, oxidative stress, drought tolerance, up-regulate expression

SONG Gui-Fang-., FAN Wei-Li, WANG Jun-Juan, WANG De-Long, WANG Shuai, ZHOU Kai, YE Wu-Wei. Cloning and Characterization of Drought-stress Responsive Gene GhGR in Gossypium hirsutum L.[J].Scientia Agricultura Sinica, 2012, 45(8): 1644-1652.

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Cloning and Characterization of Drought-stress Responsive Gene GhGR in Gossypium hirsutum L.

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