Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (5): 973-980.doi: 10.3864/j.issn.0578-1752.2012.05.019

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Isolation and Identification of an Antagonistic Bacterial Strain Against Ascosphaera apis from Honeybee Larvae Infected with Chalkbrood Disease

 LI  Jiang-Hong, ZHENG  Zhi-Yang, HONG  Shuang-Yan, QI  Xiang-Feng, LIANG  Qin   

  1. 1.福建农林大学蜂学学院，福州 350002
  • Received:2011-01-17 Online:2012-03-01 Published:2011-06-29

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Abstract: 【Objective】 The objective of this study is to explore Ascosphaera apis antagonistic bacteria for biological control of honeybee chalkbrood disease.【Method】An antagonistic bacterial strain was isolated from honeybee larvae suffered from chalkbrood disease on PDA medium plate. It was identified by combining of morphology, gram stain and 16S rDNA sequence analysis. Its inhibiting activity to Ascosphaera apis was determined in vitro and in vivo. 【Result】 The bacterium was identified as Bacillus cereus. It could inhibit the growth of Ascosphaera apis in PDA medium, but its fermentation solution could not. The death rate and development of the honeybee larvae was unchanged when feeding diet containing the bacteria suggesting that the strain was not pathogenic to honeybee. By inoculating together with the spore of Ascosphaera apis to honeybee larvae, the bacterium could not inhibit the growth of Ascosphaera apis at early infecting stage, but could effectively degrade the hyphae soon after. Ascosphaera apis could not form the offspring spore, thus lost the capability of spreading. 【Conclusion】This study provides a theoretical basis for biological control of honeybee chalkbrood disease.

Key words: chalkbrood disease, Ascosphaera apis, Bacillus cereus, antagonism, isolation

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