Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (3): 496-502.doi: 10.3864/j.issn.0578-1752.2012.03.011

• HORTICULTURE • Previous Articles     Next Articles

Genetic Relationship of 43 Cultivars of Viola tricolor and Viola cornuta Using SRAP Marker

 WANG  Tao, XU  Jin, ZHANG  Xi-Xi, ZHAO  Liang-Jun   

  1. 1.北京市园林科学研究所，北京 100102
    2.北京市农业技术推广站 ，北京 100029
    3.中国农业大学观赏园艺与园林系, 北京 100193
  • Received:2011-09-06 Online:2012-02-01 Published:2011-11-23

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Abstract: 【Objective】The molecular marker system-SRAP (sequence-related amplified polymorphism) was used to study genetic relationship and investigate genetic backgrounds of Viola tricolor and Viola cornuta for providing a theoretical basis for cultivars identification，intellectual property protection，parent selection and genetic improvement. 【Method】 Genetic relationship of 43 Viola tricolor and Viola cornuta cultivars were analyzed using SRAP marker. 【Result】 Twenty-one primer pairs selected from 88 primer pairs amplified 500 polymorphic bands with an average of 23.8 polymorphic bands per primer pair. Moreover, Jaccard´s similarity coefficient ranged from 0.62 to 0.88. The 43 cultivars could be divided into 4 groups when the genetic distance was 0.73. Viola tricolor and Viola cornuta could be isolated absolutely. The flower color was a principal factor for the cluster result. 【Conclusion】 The genetic relationship and genetic diversity of Viola tricolor and Viola cornuta could be assessed efficiently by SRAP markers.

Key words: Viola tricolor, Viola cornuta, SRAP, genetic relationship

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