Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (10): 2022-2028.doi: 10.3864/j.issn.0578-1752.2011.10.007

• PLANT PROTECTION • Previous Articles     Next Articles

SSR Mapping of Leaf Rust Resistance Gene LrY192 in Aegilops tauschii Y192

 HU  Ya-Ya, FENG  Li-Na, JI  Hong-Liu, SUN  Yi, ZHANG  Na, WEI  Xue-Jun, YANG  Wen-Xiang, LIU  Da-Qun, JIA  Ji-Zeng   

  1. 1.河北农业大学植物保护学院/河北省农作物病虫害生物防治工程技术研究中心/国家北方山区农业工程技术研究中心, 河北保定071001
    2.中国农业科学院作物科学研究所/农业部作物种质资源与生物技术重点开放实验室, 北京 100081
  • Received:2010-11-24 Online:2011-05-15 Published:2011-02-23

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Abstract: 【Objective】 The objective of this study is to find new leaf rust resistance gene in Aegilops tauschii, and provide valuable information and germplasm for wheat leaf rust resistance breeding.【Method】 Hybridization between Ae. tauschii Y192 (resistant) and Ae. tauschii Y2272 (susceptible) was carried out and the resistance of F2 population was evaluated by inoculated Puccinia triticina for investigating the resistance genes in Y192. Bulk segregation analysis and microsatellite markers on chromosome D were used to tag the resistance gene in Y192. The genetic distance was calculated by MapChart software. Gene postulation and MAS were also used to identify the resistance genes in Y192. 【Result】 F1 population derived from the crossing Y192 and Y2272 were resistant, and the ratio of resistance/susceptible was 3:1 in F2 generation. This indicated that a dominant wheat leaf rust resistance gene was presented in Y192. The gene was temporarily designated as LrY192.Three microsatellite markers Wmc245, Xgwm296 and Xgwm261 were acquired from SSR markers and linked to LrY192 with genetic distance of 4.1, 18.9, and 26.2 cM, respectively. According to the locations of the linked markers, the resistance gene was located on chromosome 2D. 【Conclusion】 Based on the chromosomal location and the resistance pattern of the gene, it is concluded that LrY192 is a novel leaf rust resistance gene, and could be selected by Wmc245.

Key words: Aegilops tauschii, wheat leaf rust, resistance gene, SSR

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