Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (20): 4258-4264.doi: 10.3864/j.issn.0578-1752.2011.20.013

• HORTICULTURE • Previous Articles     Next Articles

Establishment of Walnut Paternity Test

 ZHOU  Bei-Bei, MA  Qing-Guo, WANG  Hua, WANG  Wei, ZHANG  Mei-Yong, PEI  Dong   

  1. 1.中国林业科学研究院林业研究所
    2.山东省果树研究所
  • Online:2011-10-15 Published:2011-10-15

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Abstract: 【Objective】A simple, rapid and efficient walnut paternity method was established to resolve the problems of unknown paternal and confused parents in breeding ,which provided reference for other varieties. 【Method】'Luguo-2' was used as material. Phenotypic traits and SSR markers were conducted to analyse parentage relationship between 'Luguo-2' and its alleged parents.【Result】The phenotypes with statistics of eight nut stable expressional characters failed to get parent-child correlation results. In genotype results, the heredity of 'Shangsong-6', 'Luxiang' and 'Luguo-2' accords with Mendelian genetic regularity, there may be a paternity. PI and RCP wre analyzed by using the results of 10 SSR primer pairs, and the cumulative RCP was 99.997%. In addition, the appraisal result between ‘Slz-40’ and its known parent was consistent with the reality. 【Conclusion】'Shangsong-6' and 'Luxiang' were the parents of 'Luguo-2' with SSR clustering results supporting the conclusion. The parents of 'Luguo-2' were identified by SSR genotypes and thus providing an effective method to search and identify the parents of other trees and fruit varieties.

Key words:  walnut, phenotypes, SSR, PI, paternity testwalnut, phenotypes, SSR, PI, paternitytest

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