Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (16): 3477-3484.doi: 10.3864/j.issn.0578-1752.2011.16.022

• RESEARCH NOTES • Previous Articles     Next Articles

Optimization of Solid Fermentation of Botrytis cinerea BC7-3 Strain

ZHANG  Li-Hui, FENG  Jiang-Lan, DONG  Jin-Gao   

  1. 河北农业大学生命科学学院真菌毒素与植物分子病理学实验室
  • Received:2010-10-18 Revised:2011-03-17 Online:2011-08-15 Published:2011-05-20

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Abstract: 【Objective】 In order to enhance the production of the herbicidal substances and to furthermore separate the crude toxin, the solid fermenting conditions of BC7-3 were researched in an experiment. 【Method】 BC7-3 was fermented with solid media by using a method of orthogonal combination and different factors affecting toxin-producing was researched. The crude toxin was obtained by extracting with acetone and the herbicidal activity of crude toxin to Flanueria bidentis was bioassayed by using the method of growth inhibition. 【Result】 The medium containing millet at 5 g, sorghum at 5 g, wheat bran at 5 g and corn straw at 2.5 g were found to be the most optimum. The herbicidal activity of the crude toxin reached the strongest when BC7-3 was inoculated with the inoculum concentration of 8% in the optimum medium containing 1% sucrose, 1% ammonium mitrate and 80% water and fermented in the darkness for 15 days under the condition of 20℃. 【Conclusion】 It is suggested that solid fermentation is suitable for the production of the herbicidal substances of BC7-3.

Key words: Botrytis cinerea, Solid fermentation, Herbicidal activity, Flanueria bidentis

CLC Number: 

  • S451.1　

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