Scientia Agricultura Sinica ›› 2009, Vol. 42 ›› Issue (11): 3980-3986 .doi: 10.3864/j.issn.0578-1752.2009.11.027

• HORTICULTURE • Previous Articles     Next Articles

Molecular Markers Closely Linked to the Dominant Male Sterility Gene CDMs399-3 in Brassica oleracea

ZHANG Xin-mei, WU Jian, GUO Ai-guang, ZHANG Hui, FANG Zhi-yuan, WANG Xiao-wu
   

  1. （西北农林科技大学生命科学学院）
  • Received:2009-02-22 Revised:2009-05-04 Online:2009-11-10 Published:2009-11-10
  • Contact: WANG Xiao-wu

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Abstract:

【Objective】In this study, molecular markers linked to CDMs399-3 gene in Brassica oleracea were screened.【Method】 SRAP and SSR technologies were used to identify markers linked to CDMs399-3 based on BSA method. The bulks were formed using malesterile and malefertile individuals from a segregating BC4 population. 【Result】 A total of 8 SRAP were identified, 1 SSR and 1 SCAR markers closely linked to the male sterility gene. The two flanking markers, ENA14F-CoEm7R and 8C0909, were linked to the CDMs399-3 with a distance of 0.53 cM and 2.55 cM, respectively. ENA14F-CoEm7R was converted into a sequence characterized amplified region (SCAR) marker. 【Conclusion】 The markers developed here founded a solid basis for fining mapping of CDMs399-3 gene and were useful for accurate marker-assisted selection (MAS) of the male sterility gene.

Key words: Chinese kale, dominant male sterility, molecular marker, SRAP, SSR, SCAR

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