Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (2): 313-321 .doi: 10.3864/j.issn.0578-1752.2010.02.011

• PLANT PROTECTION • Previous Articles     Next Articles

Genome-wide Microarray Analysis of Helicoverpa armigera Larva Fed on Transgenic hpa1Xoo Cotton Leaves

MIAO Wei-guo, WU Shu-wen, SONG Cong-feng, WANG Yu, GONG Xiao-chong, ZHANG Liang, WANG Jin-sheng
   

  1. （南京农业大学植物保护学院/农业部作物病虫害监测与防控重点开放实验室）
  • Received:2009-05-18 Revised:2009-09-03 Online:2010-01-20 Published:2010-01-20
  • Contact: WANG Jin-sheng

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Abstract:

【Objective】 The objective of this study was to confirm the resistance of transgenic hpa1Xoo cotton T-34 to cotton bollworm (Helicoverpa armigera) and its resistance mechanism. 【Method】 Bioassay and 23K Oligo silkworm microarray were used to analyze the insect resistance of T-34 and globe transcriptome profile of H. armigera kept by transgenic hpa1Xoo cotton leaves comparing to untransformed cotton Z35. 【Result】 The fresh leaves of T-34 and Z35 were utilized to keep H. armigera larva, and only T-34 made H. armigera larval development become slow rather than Z35. Microarray data indicated that 872 genes in H. armigera were totally deregulated by the comparison of T-34 vs Z35. Of 872 genes, 328 genes were showed to be upregulated (ratio＞2.0), and 544 genes were indicated to be downregulated (ratio＜0.5). It was suggested that all differentially expressed genes might be classified and related to 13 biological functions, and involved in 96 biological pathways. 【Conclusion】 harpinXoo encoded by hpa1Xoo confers the resistance of transgenic hpa1Xoo cotton to H. armigera, and influences the multiple metabolism pathways in H. armigera larva.

Key words: transgenic cotton, harpinXoo, Helicoverpa armigera, 23K Oligo silkworm microarray, differentially expressed genes

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