Scientia Agricultura Sinica ›› 2005, Vol. 38 ›› Issue (10): 2153-2156 .

• RESEARCH NOTES • Previous Articles    

Establishment of the PCR Method for Detection of Suis Eperythrozoonsis

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  1. 延边大学农学院动物医学系
  • Received:2004-08-23 Revised:2005-04-30 Online:2005-10-10 Published:2005-10-10

Abstract: The 16s ribosomal RNA gene of suis eperythrozoon was amplified using gene specific primers from DNA that had been extracted from the blood of infected pig. The 936bp positive product was obtained by PCR and was cloned into the pMD18-T sector. The fragment was sequenced after restriction enzyme analysis and further defined with PCR. The sequence data demonstrated that the complete sequence was similar with the published. PCR assay for identification of eperythrozoonosis was developed. Results show that PCR assay has specificity.

Key words: Swine, Eperythrozoon, Cloning, PCR

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